ANTIBACTERIAL EFFECTS OF SARANG SEMUT (MYRMECODIA PENDANS) FRACTIONS USING THREE DIFFERENT SOLVENTS TOWARD ENTEROCOCCUS FAECALIS CPS2
Keywords:Fraction, Sarang semut, n-hexane, Ethyl acetate, Water, Enterococcus faecalis, cps2, Minimum inhibitory concentration
Objective: This study investigated the effect of antibacterial activity from sarang semut fractions with three different solvents, i.e. nonpolar (n-hexane),
semipolar (ethyl acetate), and polar (water), to determine the minimum inhibitory concentration (MIC) on Enterococcus faecalis cps2.
Methods: The fractions were extracted with a maceration method and a methanol solvent. The fractionation was performed with three groups
of solvent to obtain the n-hexane, ethyl acetate, and water fractions. The active compound from the best fraction group was identified using a
phytochemical test, gas chromatography-mass spectrophotometry, and thin-layer chromatography. Each fraction group was divided into five different
concentrations, i.e. 20%, 40%, 60%, 80%, and 100% and was assessed against E. faecalis cps2 with an agar diffusion method. Chlorhexidine 2% was
used as a positive control. The width of the inhibition zone was calculated.
Results: The ethyl acetate group had the biggest inhibition zone of 21 mm in diameter compared to n-hexane and water, which was 15 mm and
19 mm in diameter, respectively. The MIC value of the fraction with a 20% concentration of ethyl acetate was significantly different (P < 0.05) from the
n-hexane and water solvents in inhibiting the growth of E. faecalis cps2.
Conclusion: The ethyl acetate fraction of sarang semut had a greater inhibitory effect on E. faecalis cps2. In addition, the antibacterial activity of the
fraction increased with an increase in concentration.
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