PHYSICOCHEMICAL CHARACTERIZATION OF PROPRANOLOL-LOADED CHITOSAN NANOPARTICLES FOR A BUCCAL DRUG DELIVERY SYSTEM
Objective: This study aimed to characterize the physicochemical properties, including pH, zeta potential, and particle size of propranolol-loaded nanoparticles that were incorporated into a buccal transmucosal drug-delivery system.
Methods: An ionotropic gelation technique was used to formulate propranolol-loaded chitosan nanoparticles. Chitosan used as the nanoparticle base, using tripolyphosphate (TPP) as a cross-linking agent. The effects on nanoparticle physical properties, including pH, zeta potential, and particle size were examined when various chitosan [0.150-0.300 % (w/v)] and propranolol contents (0-40 mg) were used during the preparation. The effects of using chitosan solutions with different pH values on nanoparticle properties were also determined.
Results: The pH values of all nanoparticles ranged between 4.14–4.55. The zeta potentials of the prepared nanoparticles ranged between 22.6–52.6 mV, with positive charges. The nanoparticle sizes ranged from 107–140 nm, which are within the range of suitable particle sizes for transmucosal preparations.
Conclusion: The pH values, zeta potentials, and particle sizes of the nanoparticle formulations were influenced by the concentrations of chitosan and propranolol and by the pH of the initial chitosan solution. The relationships between nanoparticle properties and all factors primarily depended on the ionic charges of the components, especially chitosan. Our study provides beneficial physicochemical knowledge for the further development of chitosan-based nanoparticles containing propranolol for buccal drug delivery systems.
2. Kraisit P, Limmatvapirat S, Luangtana Anan M, Sriamornsak P. Buccal administration of mucoadhesive blend films saturated with propranolol loaded nanoparticles. Asian J Pharm Sci 2018;13:34-43.
3. Sogias IA, Williams AC, Khutoryanskiy VV. Why is chitosan mucoadhesive? Biomacromolecules 2008;9:1837-42.
4. Mohammed MA, Syeda JTM, Wasan KM, Wasan EK. An overview of chitosan nanoparticles and its application in non-parenteral drug delivery. Pharmaceutics 2017;9:1-26.
5. Hua S. Advances in nanoparticulate drug delivery approaches for sublingual and buccal administration. Front Pharmacol 2019;10:1328.
6. Kraisit P, Sarisuta N. Development of triamcinolone acetonide-loaded nanostructured lipid carriers (NLCs) for buccal drug delivery using the Box-Behnken design. Molecules 2018;23:1-14.
7. Salamat Miller N, Chittchang M, Johnston TP. The use of mucoadhesive polymers in buccal drug delivery. Adv. Drug Delivery Rev 2005;57:1666-91.
8. Kumar MNVR, Muzzarelli RAA, Muzzarelli C, Sashiwa H, Domb AJ. Chitosan chemistry and pharmaceutical perspectives. Chem Rev 2004;104:6017-84.
9. Caramella C, Ferrari F, Bonferoni MC, Rossi S, Sandri G. Chitosan and its derivatives as drug penetration enhancers. J Drug Delivery Sci Technol 2010;20:5-13.
10. Chen MC, Mi FL, Liao ZX, Hsiao CW, Sonaje K, Chung MF, et al. Recent advances in chitosan-based nanoparticles for oral delivery of macromolecules. Adv Drug Delivery Rev 2013;65:865-79.
11. Bernkop Schnurch A, Dünnhaupt S. Chitosan-based drug delivery systems. Eur J Pharm Biopharm 2012;81:463-9.
12. David KI, Jaidev LR, Sethuraman S, Krishnan UM. Dual drug-loaded chitosan nanoparticles—sugar-coated arsenal against pancreatic cancer. Colloids Surf B 2015;135:689-98.
13. Al-nemrawi NK, Alsharif SSM, Dave RH. Preparation of chitosan-tpp nanoparticles: the influence of chitosan polymeric properties and formulation variables. Int J Appl Pharm 2018;10:60-5.
14. Calvo P, Remunan Lopez C, Vila Jato JL, Alonso MJJoAPS. Novel hydrophilic chitosan?polyethylene oxide nanoparticles as protein carriers. J Appl Polym Sci 1997;63:125-32.
15. Shu XZ, Zhu KJ. The influence of multivalent phosphate structure on the properties of ionically cross-linked chitosan films for controlled drug release. Eur J Pharm Biopharm 2002;54:235-43.
16. Kraisit P. Impact of hydroxypropyl methylcellulose (HPMC) type and concentration on the swelling and release properties of propranolol hydrochloride matrix tablets usning a simplex centroid design. Int J Appl Pharm 2019;11:143-51.
17. Newton AMJ, Indana VL, Kumar J. Chronotherapeutic drug delivery of Tamarind gum, Chitosan and Okra gum controlled release colon targeted directly compressed Propranolol HCl matrix tablets and in vitro evaluation. Int J Biol Macromol 2015;79:290-9.
18. Abruzzo A, Bigucci F, Cerchiara T, Cruciani F, Vitali B, Luppi BJCp. Mucoadhesive chitosan/gelatin films for buccal delivery of propranolol hydrochloride. Carbohydr Polym 2012;87:581-8.
19. Duangjit S, Kraisit P, Luangtana Anan M. An investigation of propranolol-loaded chitosan nanoparticles for transmucosal delivery: physical characterization. Thai J Pharm Sci 2016;40:25-8.
20. Mohanraj V, Chen Y. Nanoparticles-a review. Trop J Pharm Res 2006;5:561-73.
21. Cao Y, Tan YF, Wong YS, Liew MWJ, Venkatraman S. Recent advances in chitosan-based carriers for gene delivery. Mar Drugs 2019;17:381.
22. Boonsongrit Y, Mitrevej A, Mueller BW. Chitosan drug binding by ionic interaction. Eur J Pharm Biopharm 2006;62:267-74.
23. Luangtana-Anan M, Limmatvapirat S, Nunthanid J, Chalongsuk R, Yamamoto K. Polyethylene glycol on the stability of chitosan microparticulate carrier for protein. AAPS PharmSciTech 2010;11:1376-82.
24. Ubrich N, Bouillot P, Pellerin C, Hoffman M, Maincent P. Preparation and characterization of propranolol hydrochloride nanoparticles: a comparative study. J Controlled Release 2004;97:291-300.
25. Kraisit P, Limmatvapirat S, Nunthanid J, Sriamornsak P, Luangtana-anan M. Nanoparticle formation by using shellac and chitosan for a protein delivery system. Pharm Dev Technol 2013;18:686-93.
26. Antoniou J, Liu F, Majeed H, Qi J, Yokoyama W, Zhong F. Physicochemical and morphological properties of size-controlled chitosan–tripolyphosphate nanoparticles. Colloids Surf A 2015;465:137-46.
27. Hussain Z, Katas H, Mohd Amin MCI, Kumolosasi E, Buang F, Sahudin S. Self-assembled polymeric nanoparticles for percutaneous co-delivery of hydrocortisone/hydroxytyrosol: an ex vivo and in vivo study using an NC/Nga mouse model. Int J Pharm 2013;444:109-19.
28. Hu B, Pan C, Sun Y, Hou Z, Ye H, Hu B, et al. Optimization of fabrication parameters to produce chitosan-tripolyphosphate nanoparticles for delivery of tea catechins. J Agric Food Chem 2008;56:7451-8.
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