PREPARATION AND EVALUATION OF SURFACE MODIFIED NANOPARTICLES OF CALCIUM PHOSPHATE AS EXTRACT CARRIER

  • SRITOMA BANERJEE Department of Pharmaceutical Technology, Gupta College of Technological Sciences, Ashram More, G. T. Road, Asansol 713301, West Bengal, India
  • KALYAN K. SEN Department of Pharmaceutical Technology, Gupta College of Technological Sciences, Ashram More, G. T. Road, Asansol 713301, West Bengal, India

Abstract

Objective: The aim of this study was to develop, optimize and characterize carbohydrate coated calcium phosphate nanoparticles of Chelidonium majus L. extract along with carried out in vivo study to observe activity in the liver.


Methods: Surface modified calcium phosphate nanoparticles of Chelidonium majus L. extract were developed and optimized. Extract loading and particle size were the two responses, effects on which were analyzed. Characterization studies, in vitro extract release and in vivo distribution studies were carried out. Also in vivo histopathological analysis was carried out to observe effects of extract loaded nanoparticles in liver of wistar albino rats in paracetamol, rifampicin-isoniazid, cisplatin and carbon tetrachloride-induced hepatotoxicity.


Results: Pareto chart and surface response curve indicated that sonication time, the concentration of lactose and concentration of extract were important factors affecting particle size and extract loading. ANOVA was performed and obtained data pointed out that model was significant for both responses. Particle size and zeta potential results indicated the stability of prepared nanoparticles along with extract was loaded (37.22 %) satisfactorily on coated cores. Characterization studies indicated no interaction between the components and also extract release demonstrated diffusion-controlled mechanism. These extract loaded nanoparticles were largely found in the liver than heart, lungs. Hepatoprotective activity of nanoparticles of the extract was confirmed by correlating histopathology results of normal, toxic, silymarin treated, extract-treated and formulation treated groups.


Conclusion: Lactose coated nanoparticles of calcium phosphate proved to be excellent carriers of plant extract. These nanoparticles efficiently targeted liver and generated cellular protective action in hepatic damage.

Keywords: Calcium phosphate, Surface modification, Statistical optimization, Chelidonium majus L., Distribution, Hepatoprotective

References

1. Kossovsky N, Gelman A, Sponsler EE, Hnatyszyn HJ, Rajguru S, Torres M, et al. Surface-modified nanocrystalline ceramics for drug delivery applications. Biomaterials 1994;15:1201–7.
2. Khopade AJ, Khopade S, Jain NK. Development of haemoglobin aquasomes from spherical hydroxyapatite cores precipitated in the presence of poly(amidoamine) dendrimer. Int J Pharm (Amsterdam, Neth) 2002;241:145-54.
3. Patil S, Pancholi SS, Agrawal S, Agrawal GP. Surface-modified mesoporous ceramics as a delivery vehicle for haemoglobin. Drug Delivery 2004;11:193-9.
4. Cherian AK, Rana AC, Jain SK. Self-assembled carbohydrate-stabilized ceramic nanoparticles for the parenteral delivery of insulin. Drug Dev Ind Pharm 2000;26:459–69.
5. Kossovsky N, Gelman A, Hnatyszyn HJ, Rajguru S, Garrell RL, Torbati S, et al. Surface-modified diamond nanoparticles as antigen delivery vehicles. Bioconjugate Chem 1995;6:507-11.
6. Kossovsky N, Gelman A, Sponsler E, Rajguru S, Torres M, Mena E, et al. Preservation of surface-dependent properties of viral antigens following immobilization on particulate ceramic delivery vehicles. J Biomed Mater Res 1995;29:561–73.
7. Goyal AK, Khatri K, Mishra N, Mehta A, Vaidya B, Tiwari S, et al. Aquasomes-a nanoparticulate approach for the delivery of antigen. Drug Dev Ind Pharm 2008;34:1297-305.
8. Kommineni S, Ahmad S, Vengala P, Subrahmanyam CVS. Sugar coated ceramic nanocarriers for the oral delivery of hydrophobic drugs: formulation, optimization and evaluation. Drug Dev Ind Pharm 2012;38:577-86.
9. Vengala P, Aslam S, Subrahmanyam CVS. Development and in vitro evaluation of ceramic nanoparticles of piroxicam. Latt Am J Pharm 2013;32:1124-30.
10. Vengala P, Dintakurthi S, Subrahmanyam CVS. Lactose coated ceramic nanoparticles for oral drug delivery. J Pharm Res 2013;7:540-5.
11. Rawat M, Singh D, Saraf S, Saraf S. Development and in vitro evaluation of alginate gel-encapsulated, chitosan-coated ceramic nanocores for oral delivery of enzyme. Drug Dev Ind Pharm 2008;34:181-8.
12. Goyal AK, Rawat A, Mahor S, Gupta PN, Khatri K, Vyas SP. Nanodecoy system: a novel approach to design hepatitis B vaccine for immunopotentiation. Int J Pharm (Amsterdam, Neth) 2006;309:227-33.
13. Kaur K, Kush P, Pandey RS, Madan J, Jain UK, Katare OP. Stealth lipid-coated aquasomes bearing recombinant human interferon-?-2b offered prolonged release and enhanced cytotoxicity in ovarian cancer cells. Biomed Pharmacother 2015;69:267-76.
14. Gilca M, Gaman L, Panait E. Chelidonium majus–an integrative review: traditional knowledge versus modern findings. Forschende Komplementärmedizin 2010;17:241-8.
15. Zielinska S, Jezierska Domaradzka A, Wójciak Kosior M. Greater celandine’s ups and downs–21 centuries of medicinal uses of Chelidonium majus from the viewpoint of today’s pharmacology. Front Pharmacol 2018;9:1-29.
16. Banerjee S, Sen KK. Qualitative and quantitative evaluation study along with method development and validation for UV spectrophotometric analysis of Chelidonium majus L. extract. J Pharmacogn Phytochem 2019;8:4629-36.
17. Prabu P, Chaudhari AA, Dharmaraj N, Khil MS, Park SY, Kim HY. Preparation, characterization, in vitro drug release and cellular uptake of poly(caprolactone) grafted dextran copolymeric nanoparticles loaded with an anticancer drug. J Biomed Mater Res, Part A 2008;90:1128-36.
18. Kumar R, Nagarwal RC, Dhanawat M, Pandit JK. In vitro and in vivo study of indomethacin loaded gelatin nanoparticles. J Biomed Nanotechnol 2011;7:1-9.
19. Anuradha, Joshi JC, Gulati K, Ray A, Roy I. Fluorophore-doped calcium phosphate nanoparticles for non-toxic biomedical applications. RSC Adv 2014;4:40449-55.
20. Zavoi S, Fetea F, Ranga F, M Pop R, Baciu A, Socaciu C. Comparative fingerprint and extraction yield of medicinal plant phenolics with hepatoprotective potential, as determined by UV-vis and FT-MIR spectroscopy. Not Bot Horti Agrobot Cluj-Napoca 2011;39:82-9.
21. Dobrucka R, Dlugaszewska J, Kaczmarek M. Cytotoxic and antimicrobial effects of biosynthesized ZnO nanoparticles using of Chelidonium majus extract. Biomed Microdevices 2017;20:5.
22. Srividya G, Adilaxmamma K, Srilatha CH. Protective effect of Acorus calamus rhizome in paracetamol exposure induced hepatotoxicity in rats: biochemical and histopathological study. Int J Curr Pharm Res 2018;10:7-10.
23. Sitorus P, Nerdy N. Hepatoprotective activity of Vernonia amygdalina leaf ethanolic extract in white rats induced by paracetamol. Asian J Pharm Clin Res 2018;11:562-4.
24. Garcia Nino WR, Zazueta C. Ellagic acid: pharmacological activities and molecular mechanisms involved in liver protection. Pharmacol Res 2015;97:84-103.
25. Bais B, Saiju P. Ameliorative effect of Leucas cephalotes extract on isoniazid and rifampicin induced hepatotoxicity. Asian Pac J Trop Biomed 2014;4(Suppl 2):S633-8.
26. Eminzade S, Uraz F, Izzettin FV. Silymarin protects the liver against toxic effects of anti-tuberculosis drugs in experimental animals. Nutr Metab (Lond) 2008;5:1-8.
27. Hussain T, Gupta RK, Sweety K. Evaluation of the antihepatotoxic potential of Solanum xanthocarpum fruit extract against antitubercular drugs induced hepatopathy in experimental rodents. Asian Pac J Trop Biomed 2012;2:454-60.
28. Abdelmeguid NE, Chmaisse HN, Zeinab NSA. Silymarin ameliorates cisplatin-induced hepatotoxicity in rats: histopathological and ultrastructural studies. Pak J Biol Sci 2010;13:463-79.
29. Mitra S, Sur RK (deceased), Roy A, Mukherjee AS. Effect of Chelidonium majus L. on experimental hepatic tissue injury. Phytother Res 1996;10:354–6.
30. Rajalingam D, Varadharajan R, Palani S. Evaluation of hepatoprotective and antioxidant effect of Combretum albidum G. Don against ccl4 induced hepatotoxicity in rats. Int J Pharm Pharm Sci 2016;8:218-23.
31. Mushtaq A, Masoodi MH, Wali AF, Ganai BA. Multiple treatments of eremurus himalaicus extracts ameliorates carbon tetrachloride-induced liver injury in rats. Int J Pharm Pharm Sci 2016;8:24-7.
Statistics
53 Views | 82 Downloads
Citatons
How to Cite
BANERJEE, S., & SEN, K. K. (2020). PREPARATION AND EVALUATION OF SURFACE MODIFIED NANOPARTICLES OF CALCIUM PHOSPHATE AS EXTRACT CARRIER. International Journal of Applied Pharmaceutics, 12(4), 248-257. https://doi.org/10.22159/ijap.2020v12i4.38126
Section
Original Article(s)