DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR THE DETERMINATION OF METHYLISOTHIAZOLINONE AND METHYLCHLOROISOTHIAZOLINONE IN COSMETIC PRODUCTS USING MATRIX SOLID-PHASE DISPERSION COUPLED TO GAS CHROMATOGRAPHY-MASS SPECTROMETRY

Authors

  • NURINA PRAPURANDINA Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia
  • HARMITA HARMITA Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia
  • HERMAN SURYADI Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2021v13i2.40058

Keywords:

Methylisothiazolinone, Methylchloroisothiazolinone, Matrix solid-phase dispersion, Gas chromatography-mass spectrometry, Validation

Abstract

Objective: This study was to develop the first simultaneous method for quantification of MI and MCI by using matrix solid-phase dispersion (MSPD) as an extraction technique followed by gas chromatography-tandem mass spectrometry (GC-MS) in cosmetic products to support that law enforcement.

Methods: The MI and MCI were extracted from the cosmetic sample by using matrix solid-phase dispersion technique with alumina as solid sorbent and ethyl acetate as eluent. After being isolated, MI and MCI from the samples were analyzed using GC-MS equipped with DB-5MS capillary column.

Results: The validated method for both leave-on and rinse-off cosmetic showed that MI and MCI recoveries were between 97.87-103.15 %, relative standard deviation (RSD) values were lower than 11%, and limit of quantitation (LOQ) values for the leave-on product were 0.96 µg/ml and 1.95 µg/ml and for rinse-off products were 0.56 µg/ml and 1.49 µg/ml for MI and MCI, respectively.

Conclusion: This purposed analytical method for determining MI and MCI in cosmetic products using MSPD-GC-MS complies with the validation acceptance criteria.

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Published

07-03-2021

How to Cite

PRAPURANDINA, N., HARMITA, H., & SURYADI, H. (2021). DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR THE DETERMINATION OF METHYLISOTHIAZOLINONE AND METHYLCHLOROISOTHIAZOLINONE IN COSMETIC PRODUCTS USING MATRIX SOLID-PHASE DISPERSION COUPLED TO GAS CHROMATOGRAPHY-MASS SPECTROMETRY. International Journal of Applied Pharmaceutics, 13(2), 140–147. https://doi.org/10.22159/ijap.2021v13i2.40058

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