The ANTICHOLESTEROL ACTIVITY OF BETULINIC ACID AND STIGMASTEROL ISOLATED FROM THE LEAVES OF SUNGKAI (Paronema canescens)
Objective:The purpose of this study were to isolation and evaluate anticholesterol activity these compound from the leaves of Paronema canescens.
Methods:These compounds were isolated via chromatography using silica gel as the stationary phase. Their structures were revealed using IR and 1D NMR data and by comparing their NMR data with the data in the literature. The anticholesterol activity of compounds was tested using photometry via the Liebermann–Burchard reaction.Results:Betulinic acid (1) and stigmasterol (2) were isolated from the leaves extracts of sungkai (P. canescens). The betulinic acid (1) that was isolated showed anticholesterol activity with an IC50 value of 60.53 µg/ml, stigmasterol (2) 222.32 µg/ml, while the standard anticholesterol compound, simvastatin had an IC50 of 24.68 µg/ml.Conclusion:The stigmasterol (2) has previously been reported from P. canescens while that of betulinic acid (1) is reported here for the first time and the identification of its anticholesterol activity
Kusriani RH, Nawawi A, Turahman T. Antibacterial activity test extract and fractions the stem bark and leaves of sungkai (Peronema Canescens Jack) on Staphylococcus aureus Atcc 25923 and Escherichia coli ATCC 25922. J Pharm Galenika 2015;2: 8-14
Yani AP, Putranto MH. Examination of the sungkai’s young leaf extract (Peronema canescens) as an antipiretic, immunity, antiplasmodium and teratogenity in mice (Mus.muculus). Int J Sci and Eng 2014;7: 30-34
Yustian I, Muharni, Zulaicha S, Arbi M. Special Research on the Exploration of Ethnomedicine and Local Community Medicinal Plants in Indonesia (Ethnic Musi II). Palembang. Ministry of Health Republic of Indonesia;2012
Carmena R, Duriez P, Fruchart JC. Atherogenic lipoprotein particles in atherosclerosis. Circulation 2004;109:2–7.
Finn AV, Nakano M, Narula J, Kolodgie FD, Virmani R. Concept of vulnerable/unstable plaque Arterioscler. Thromb. Vasc Biol 2010; 30: 1282-92.
Prasiwi D, Sundaryono A, Handayani D. Activity of Ethanol Fraction from Peronema canescens Leaf Extract on Growth Rate of Plasmodium berghei. J Education Chem 2018;2:25 –32
Ibrahim A, Kuncoro H. Identification secondary metabolites and antibacterial activity of sungkai (Peronema Canescens Jack.) Leaf extract against some pathogenic bacteria. J Trop Pharm Chem 2012;2: 8-18
Simanjuntak P., Chemical study of the glycoside compounds of the sungkai Peronema canescens (Verbenaceae). Ind J of Appl Chem 1996; 6: 8-12.
Kitagawa I, Simanjuntak P, Hori K, Nagami B, Mahmud T, Shibuya H, Kobayashi M. Indonesian medicinal plants. VII seven new clerodane-type diterpenoids, peronemins A2, A3, B1, B2, B3, C1 and D1 from the leaves of Peronema canescens (Vebenaceae). J Chem and Pharm Bull 1994;42:1050-5
Muharni, Fitrya, Farida, S. 2017. Antibacterial Activity Test Ethanol Extract of Medicinal Plants of the Musi Tribe in Musi Regency, Banyuasin, South Sumatra. Ind Pharm J 2017;7:127-135.
Musa WJA, Situmeang B, Sianturi J. Anti-cholesterol triterpenoid acids from Saurauia vulcani Korth. (Actinidiaceae). Int J Food Prop 2019; 22:1439-44
Ragasa CY, Alimboyoguen AB, Shen CC. Antimicrobial triterpenes from Dillenia philippinensis. Philip Scient 2009;46:78-87.
Khaliq S, Volk FJ, Frahm AW.. Phytochemical investigation of Perovskia abrotanoides. Planta Medica 2017;73:77–83.
Li X, Li CW, Cui CB, Li MM, Fan M. Chemical Constituents of Dillenia kerrii and their activities on antitumor and anti-hypoxia in vitro. Chin J Med Chem 2009;19: 130–4.
Nick A, Wright AD, Sticher O, Rali T. Antibacterial triterpenoid acids from Dillenia papuana. J Nat Prod 1994;57:1245–50
Rzeski W, Stepulak A, Szymanski M, Sifringer M, Kakzor J, Wejksza K, Zdzisinstca B, Kandefer-Szerszen M. Betulinic acid decreases expression of bcl-2 and cyclin D1, inhibits proliferation, migration and induces apoptosis in cancer cells. Naunyn Schmiedebergs Arch Pharmacol 2006;374:11–20.
Hashimoto F, Kashiwada Y, Cosentino LM, Chan CH, Garret PE, Lee KA. Anti-AIDS agents—XXVII. Synthesis and anti–HIV activity of betulinic acid and dihydrobetulinic acid derivatives. Bioorg Med Chem 1997;5:2133–43.
Cichewicz RH, Kouzi SA. Chemistry, biological activity, and chemotherapeutic potential of betulinic acid for the prevention and treatment of cancer and HIV infection. Med Res Rev 2004;24:90–114.
Ahmed Y, Rahman S, Akther P, Islam F, Rahman M, Yaakob Z. Isolation of Steroids from n-Hexane Extract of The Leaves of Saurauia roxburghii Int Food Res J 2013;20:2939-43.
Tisnadjaja D, Hidayat SL, Sumirja S, Simanjuntak P. Pengkajian kandungan fitosterol pada tanaman kedawung (Parkia roxburgii G. Don.). Biodiversitas 2006;7: 21-4.
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