DEVELOPMENT AND OPTIMIZATION OF MANNOSYLATED NARINGENIN LOADED TRANSFERSOMES USING RESPONSE SURFACE METHODOLOGY FOR SKIN CARCINOMA
Naringenin is a potent antioxidant, having remarkable reactive oxygen species scavenging potential andabundantly found in citrus fruits. There are so many studies have been already reported based onnaringenin outstanding action towards inhibiting the UV induced skin carcinoma. The mannose receptor is a lectin binding receptor which is highly expressed in macrophages in cancerous cellsand D Mannosamine HCl are ligand which has affinity towards mannose receptors. Therefore keeping both the concepts in mind our objective is to designand optimize the mannosylated naringenin loaded transfersomes (O-MA-NgTfs) for macropahges targeting. The Box Behnken with 3D surface response design graph was employed to optimize the formulation. The transfersomes was prepared by modified thin film hydration method and optimized formulation was evaluated by different parameters like surface morphology, entrapment efficiency, zeta potential, FTIR, DSC, XRD, invitro drug release, ex vivo permeation study, skin retention and deposition study and the cellular uptake by HaCaT macrophages cells. On the basis of findings the study revealed that the prepared formulation has characteristic potential for targeting and the concept of ligand directed nanocarrier formulation was imparts synergistic effect against UV- induced skin carcinoma.
This work is licensed under a Creative Commons Attribution 4.0 International License.