LUEM PUA RICE EXTRACT ATTENUATES THE SEVERITY OF DEXTRAN SULFATE SODIUMINDUCED ULCERATIVE COLITIS IN RATS THROUGH CHOLINOMIMETIC EFFECTS
Objective: The objective of the study was to investigate the effect of aqueous extract of unpolished dark purple glutinous Thai rice variety Luem Pua
(LP) in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in rat and the possible cholinomimetic effects of the extract.
Methods: The effect of LP extract (0.5, 1, or 1.5 mg/ml final concentration) on ileum contraction was tested using isolated guinea pig ileum. Certain
agonists (acetylcholine, tetramethylammonium, and clonidine) and antagonists (hexamethonium chloride and atropine) were studied to determine
the cholinomimetic effect of the extract. The effects of LP extract (5 g/kg/day) in DSS-induced UC model (drinking water was replaced with 3%
DSS in water for 7 days) in rat were evaluated. On each day of treatment, the change of disease activity index (DAI) was recorded. At the end of the
experiments, rats were terminated and disease severity expressed as DAI, colon length, and spleen weight were determined.
Results: LP extract at the concentration of 0.5, 1, and 1.5 mg/ml (final concentration) could contract the ileum in a dose-dependent manner and be
blocked completely by atropine. Oral administration of LP extract could significantly attenuate the severity of DSS-induced UC as seen by the reduction
of DAI, colon length, and spleen weight.
Conclusion: Results in isolated guinea pig ileum suggest that LP might contain active substance that could activate muscarinic receptors. In addition
to antioxidant activity, through activation of muscarinic receptor, might explain the protective effects of LP extract against DSS-induced UC in rats.
cholinergic activation of a vagus nerve-to-spleen circuit alleviates
experimental colitis. Mucosal Immunol 2014;7:335-47.
2. Di Giovangiulio M, Verheijden S, Bosmans G, Stakenborg N,
Boeckxstaens GE, Matteoli G, et al. The neuromodulation of the
intestinal immune system and its relevance in inflammatory bowel
disease. Front Immunol 2015;6:590.
3. Kolgazi M, Uslu U, Yuksel M, Velioglu-Ogunc A, Ercan F, Alican I,
et al. The role of cholinergic anti-inflammatory pathway in acetic
acid-induced colonic inflammation in the rat. Chem Biol Interact
4. Shifrin H, Nadler-Milbauer M, Shoham S, Weinstock M. Rivastigmine
alleviates experimentally induced colitis in mice and rats by acting at
central and peripheral sites to modulate immune responses. PLoS One
5. Dhawan S, Hiemstra IH, Verseijden C, Hilbers FW, Te Velde AA,
Willemsen LE, et al. Cholinergic receptor activation on epithelia
protects against cytokine-induced barrier dysfunction. Acta Physiol
6. Brinkman DJ, ten Hove AS, Vervoordeldonk MJ, Luyer MD, de
Jonge WJ. Neuroimmune interactions in the gut and their significance
for intestinal immunity. Cells 2019;8:670.
7. Suwannalert P, Rattanachitthawat S. High levels of phytophenolics
and antioxidant activities in Oryza sativa unpolished Thai rice strain of
Leum Phua. Trop J Pharm Res 2011;10:431-6.
8. Srisuwan S, Arkaravichien T, Mahatheeranont S, Puangsombat P,
Seekhaw P, Noenplab AN, et al. Effects of aqueous extract of unpolished
dark purple glutinous rice var Luem Pua on ROS in SK-N-SH cells
and scopolamine-induced memory deficit in mice. Trop J Pharm Res
9. Ochillo RF, Tsai CS, Tsai MH. Mechanism of action of muscarine on
the longitudinal muscle of the guinea-pig isolated ileum. Br J Pharmacol
10. Pengkumsri N, Sivamaruthi BS, Sirilun S, Suwannalert P, Rodboon T,
Prasitpuriprecha C, et al. Dietary supplementation of Thai black rice
bran extract and yeast beta-glucan protects the dextran sodium sulphate
mediated colitis induced rat. Rsc Adv 2017;7:396-402.
11. Nagib MM, Tadros MG, ElSayed MI, Khalifa AE. Anti-inflammatory
and anti-oxidant activities of olmesartan medoxomil ameliorate
experimental colitis in rats. Toxicol Appl Pharmacol 2013;271:106-13.
12. Fujii W, Hori H, Yokoo Y, Suwa Y, Nukaya H, Taniyama K, et al.
Beer congener stimulates gastrointestinal motility via the muscarinic
acetylcholine receptors. Alcohol Clin Exp Res 2002;26:677-81.
13. Edwards CA, Havlik J, Cong W, Mullen W, Preston T, Morrison DJ,
et al. Polyphenols and health: Interactions between fibre, plant
polyphenols and the gut microbiota. Nutr Bull 2017;42:356-60.
14. Badary OA, Awad AS, Sherief MA, Hamada FM. In vitro and in vivo
effects of ferulic acid on gastrointestinal motility: Inhibition of cisplatininduced
delay in gastric emptying in rats. World J Gastroenterol
15. Ghia JE, Blennerhassett P, Collins SM. Impaired parasympathetic
function increases susceptibility to inflammatory bowel disease in a
mouse model of depression. J Clin Invest 2008;118:2209-18.
16. Meroni E, Stakenborg N, Viola MF, Boeckxstaens GE. Intestinal
macrophages and their interaction with the enteric nervous system
in health and inflammatory bowel disease. Acta Physiol (Oxf)
17. Rosas-Ballina M, Tracey KJ. Cholinergic control of inflammation. J
Intern Med 2009;265:663-79.
18. Guan G, Lan S. Implications of antioxidant systems in inflammatory
bowel disease. Biomed Res Int 2018;2018:290179.
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