EFFECT OF LYOPHILIZED JUICE OF PUNICA GRANATUM L. IN ANTINOCICEPTIVE AND INFLAMMATORY PAIN

  • JOSE ANTONIO GUERRERO-SOLANO Health Sciences Institute, Autonomous University of Hidalgo State, Circuito Ex Hacienda La Concepción, km 1.5. C.P. 42160, San Agustín Tlaxiaca, Hidalgo, Mexico
  • MIRANDELI BAUTISTA Health Sciences Institute, Autonomous University of Hidalgo State, Circuito Ex Hacienda La Concepción, km 1.5. C.P. 42160, San Agustín Tlaxiaca, Hidalgo, Mexico
  • NELLY DEL SOCORRO CRUZ-CANSINO Health Sciences Institute, Autonomous University of Hidalgo State, Circuito Ex Hacienda La Concepción, km 1.5. C.P. 42160, San Agustín Tlaxiaca, Hidalgo, Mexico
  • ALEJANDRO CHEHUE-ROMERO Health Sciences Institute, Autonomous University of Hidalgo State, Circuito Ex Hacienda La Concepción, km 1.5. C.P. 42160, San Agustín Tlaxiaca, Hidalgo, Mexico
  • OSMAR ANTONIO JARAMILLO-MORALES Department of Nursing and Obstetrics, Division of Life Sciences, University of Guanajuato, Ex Hacienda el Copal, km 9 Irapuato-Silao Highway Ap 311, Irapuato, Guanajuato 36500, Mexico

Abstract

Objective: The aim of this study was to explore the analgesic activity of lyophilized juice of Punica granatum L., obtained from Hidalgo, Mexico, in the
formalin test.
Methods: We extracted the juice manually, filtered it and then dried down in a lyophilizer machine. We evaluated the antinociceptive effect of
lyophilized juice from pomegranate in the formalin test (2%) in male Wistar rats (180–200 g body weight). Thirty minutes before the test, a dose of
316 mg/kg (lyophilized juice) and acetylsalicylic acid as reference drug (100 mg/kg) both were administered intragastrically (i.g.).
Results: The oral administration of lyophilized juice of pomegranate showed a significant decrease in the number of flinches in the temporal course
and a significant antinociceptive effect in nociceptive and inflammatory pain compared with the vehicle. In the same way, this effect appeared with the
drug of reference (acetylsalicylic acid 100 mg/kg i.g). Furthermore, it was shown that juice had a 34% of antinociception on overall effect versus vehicle.
Conclusion: The results suggest that lyophilized juice of pomegranate has antinociceptive effect in nociceptive and inflammatory pain. Therefore, this
study supports the possible use of this lyophilized juice of pomegranate in the treatment of pain.

Keywords: Antinociceptive, Lyophilized juice, Punica granatum L, Pomegranate, Formalin test

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GUERRERO-SOLANO, J. A., BAUTISTA, M., CRUZ-CANSINO, N. D. S., CHEHUE-ROMERO, A., & JARAMILLO-MORALES, O. A. (2021). EFFECT OF LYOPHILIZED JUICE OF PUNICA GRANATUM L. IN ANTINOCICEPTIVE AND INFLAMMATORY PAIN. International Journal of Applied Pharmaceutics, 13(1), 17-19. https://doi.org/10.22159/ijap.2021.v13s1.Y0055
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