PHYTOCHEMICAL COMPOSITION AND ANTIINFLAMMATORY ACTIVITY OF NELUMBO NUCIFERA GAERTN

Authors

  • WARACHATE KHOBJAI Department of Clinical Chemistry, Faculty of Medical Technology, Nation University, Lampang, Thailand
  • NAKUNTWALAI WISIDSRI Department of Thai Traditional Medicine, Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand
  • KHEMJIRA JARMKOM Department of Thai Traditional Medicine, Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand
  • SURACHAI TECHAOEI Department of Thai Traditional Medicine, Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand

DOI:

https://doi.org/10.22159/ijap.2021.v13s1.Y0102

Keywords:

Nelumbo nucifera Gaertn, Nitric oxide, RAW2647 macrophage cell, Cytotoxicity

Abstract

Objective: Inflammation is a process of injuries caused by physical, chemical, and biological factors. Nitric oxide (NO) plays an important role in the
regulation of various pathological and pathophysiological processes. Overproduction of NO induces tissue damage associated with acute and chronic
inflammations. This study was conducted to determine the phytochemical composition and the NO inhibitory properties of Nelumbo nucifera extracts
in lipopolysaccharide(LPS)-stimulated macrophage cell line.
Methods: The dried leaf, stalk, and flower materials of roseum plenum and album plenum (AP) were extracted with 95% ethanol solvents. The
phytochemical compounds of the extraction were analysed by gas chromatography-mass spectrometry. The cytotoxic assay of extracts against
macrophage cells was conducted using resazurin. The NO was determined using LPS-induced RAW264.7 cells to measure inhibitory activity of extract
on the production of NO.
Results: The extracts from Lotus, which exhibited the non-cytotoxic to the RAW264.7 cells. The AP-stalk extracts were capable to reduce the NO level
in LPS-activated RAW264.7 cells. GC-MS analysis of AP-stalk extraction revealed pharmacologically active compounds.
Conclusion: The results conduct that the AP-stalk extract effectively inhibited the NO production and may be useful in preventing inflammatory
diseases mediated by excessive production of NO. Bio-active phytoconstituents from AP stalk extract could potentially be used for anti-inflammation.
These data also suggest that AP-stalk extract may serve as a good indicator of the pharmacological activities of medicinal plants.

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References

1. Schottenfeld D, Beebe-Dimmer J. Alleviating the burden of cancer:
A perspective on advances, challenges, and future directions. Cancer
Epidemiol Biomarkers Prev 2006;15:2049-55.
2. Coussens LM, Werb Z. Inflammation and cancer. Nature 2002;420:860-7.
3. Huang SS, Chiu CS, Chen HJ, Hou WC, Sheu MJ, Lin YC, et al.
Antinociceptive activities and the mechanisms of anti-inflammation
of asiatic acid in mice. J Evid Based Complement Altern Med
2011;2011:895857.
4. Fang SC, Hsu CL, Yen GC. Anti-inflammatory effects of phenolic
compounds isolated from the fruits of Artocarpus heterophyllus. J Agr
Food Chem 2008;56:4463-8.
5. Joo T, Sowndhararajan K, Hong S, Lee J, Park SY, Kim S, et al.
Inhibition of nitric oxide production in LPS-stimulated RAW264.7 cells
by stem bark of Ulmus pumila L. Saudi J Biol Sci 2014;21:427-35.
6. Nakagawa T, Yokozawa T. Direct scavenging of nitric oxide and
Table 1: The NO concentration under variable AP-stem
concentrations, compared with vehicle as negative control
superoxide by green tea. Food Chem Toxicol 2002;40:1745-50.
7. Taira J, Nanbu H, Ueda K. Nitric oxide-scavenging compounds in
Agrimonia pilosa Ledeb on LPS-induced RAW264.7 macrophages.
Food Chem 2009;115:1221-7.
8. Pacher P, Beckman JS, Liaudet L. Nitric oxide and peroxynitrite in
health and disease. Physiol Rev 2007;87:315-24.
9. Moncada S, Palmer RM, Higgs EA, Nitric oxide: Physiology,
pathophysiology, and pharmacology. Pharmacol Rev 1991;43:109-42.
10. Nicholas C, Batra S, Vargo MA, Voss OH, Gavrilin MA, Wewers MD,
et al. Apigenin blocks lipopolysaccharide-induced lethality in vivo
and proinflammatory cytokines expression by inactivating NFkappaB
through the suppression of p65 phosphorylation. J Immunol
2007;179:7121-7.
11. Paudel KR, Panth N. Phytochemical profile and biological activity
of Nelumbo nucifera. Evid Based Complement Altern Med
2015;2015:789124.
12. Liu CP, Tsai WJ, Lin YL, Liao JF, Chen CF, Kuo YC. The extracts
from Nelumbo nucifera suppress cell cycle progression, cytokine
genes expression, and cell proliferation in human peripheral blood
mononuclear cells. Life Sci 2004;75:699-716.
13. Dno Y, Hattori E, Fukaya Y, Imai S, Dhizum Y. Anti-obesity effect of
Nelumbo nucifera leaves extract in mice and rats. J Ethnopharmacol
2006;106:238-44.
14. Wang QZ, Pen GH, Jin Y, Li J, Yan SL. Extraction of polyphenol from lotus
roots and its enzymatic browning substrate. J Anal Sci 2004;20:38-40.
15. Yang D, Zhang Q, Ren G, Ying T. A comparative study on antioxidant
activity of different parts of lotus (Nelumbo nuficera Gaertn) rhizome.
Food Sci Technol 2017;37:135-8.
16. Yen GC, Duh PD, Huang DW, Hsu CL, Fu TY. Protective effect of
pine (Pinus morrisonicola Hay.) needle on LDL oxidation and its antiinflammatory
action by modulation of iNOS and COX-2 expression
in LPS-stimulated RAW264.7 macrophages. Food Chem Toxicol
2008;46:175-85.
17. Mohsen SM, Ammar AS. Total phenolic contents and antioxidant
activity of corn tassel extracts. Food Chem 2009;112:595-8.
18. Bancos S, Tsai DH, Hackley V, Weaver JL, Tyner KM. Evaluation of
viability and proliferation profiles on macrophages treated with silica
nanoparticles in vitro via plate-based, flow cytometry, and coulter
counter assays. Int Sch Res Notices 2012;2012:454072.
19. Kim JM, Jung HA, Choi JS, Min BS, Lee NG. Comparative
analysis of the anti-inflammatory activity of Huang-lian extracts in
lipopolysaccharide-stimulated RAW264.7 murine macrophage-like cells
using oligonucleotide microarrays. Arch Pharm Res 2010;33:1149-57.
20. Singariya P, Mourya1 KK, Kumar P. Gas chromatography-mass
spectrometric analyses of acetone extract of Marwardhaman grass for
bioactive compounds. Plant Arch 2015;15:1065-74.
21. Terao J. Dietary flavonoids as antioxidants. Forum Nutr 2009;61:87-94.
22. Lander HM, Jacovina AT, Davis RJ, Tauras JM. Differential activation
of mitogen-activated protein kinases by nitric oxide-related species. J
Biol Chem 1996;272:19705-9.
23. Kopydlowski KM, Salkowski CA, Cody MJ, van Rooijen N, Major J,
Hamilton TA, et al. Regulation of macrophage chemokine expression by
lipopolysaccharide in vitro and in vivo. J Immunol 1999;163:1537-44.
24. Bingle L, Brown NJ, Lewis CE. The role of tumour-associated
macrophages in tumour progression: Implications for new anticancer
therapies. J Pathol 2002;196:254-65.
25. Patel RP, McAndrew J, Sellak H, White CR, Jo H, Freeman BA, et al.
Biological aspects of reactive nitrogen species. Biochim Biophys Acta
1999;1411:385-400.
26. Musa AM, Cooperwood SJ, Khan MO. A review of coumarin
derivatives in pharmacotherapy of breast cancer. Curr Med Chem
2008;15;26:2664-79.
27. Dinarello CA. Anti-inflammatory agents present and future. Cell
2010;140;6:935-50.
28. Szliszka E, Skaba D, Czuba ZP, Krol W. Inhibition of inflammatory
mediators by neobaisoflavone in activated RAW264.7 macrophages.
Molecules 2011;16:3701-12.

Published

31-01-2021

How to Cite

KHOBJAI, W., WISIDSRI, N., JARMKOM, K., & TECHAOEI, S. (2021). PHYTOCHEMICAL COMPOSITION AND ANTIINFLAMMATORY ACTIVITY OF NELUMBO NUCIFERA GAERTN. International Journal of Applied Pharmaceutics, 13(1), 42–46. https://doi.org/10.22159/ijap.2021.v13s1.Y0102

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