PHYTONUTRIENT SCREENING BY GAS CHROMATOGRAPHY–MASS SPECTROMETRY AND ANTI-AGING PROPERTIES OF GLYCOSMIS PENTAPHYLLA (RETZ.) DC. (SOM-CHUEN) EXTRACTS
Keywords:Glycosmis pentaphylla (Retz) DC, Anti-aging, Phytonutrients, Anti-Glycation, Matrix Mettalloproteinase-1
Objective: The aims of this study were to screen the phytonutrient constituents and investigate their anti-aging property of leaves and bark of
Glycosmis pentaphylla (Retz.) DC. (GP).
Methods: GP is the medicinal plant which used as traditional medicine. Glycosmis pentaphylla leaves (GPL) and Glycosmis pentaphylla bark (GPB) of
GP were extracted with ethanol. The gas chromatography–mass spectrometry was used for phytonutrients analysis. The anti-aging properties were
performed using ABTS assay, deoxyribose degradation assay, bovine serum albumin (BSA)-fructose model, and matrix metalloproteinase 1 (MMP-1)
Results: The important phytonutrients of GPL were α-tocopherol, linolenic acid, squalene, stigmasterol, and β-amyrin and for GPB were α-tocopherol,
phytol, campesterol, stigmasterol, dictamine, and γ-sitosterol. The percentage inhibition of GPL and GPB extracts at various concentrations was
between 16.57–76.05 and 20.66-78.81 by ABTS assay, 68.24–90.06 and 73.83–96.64% by deoxyribose degradation assay, 4.24–99.98 and 54.81–
99.94 by BSA-fructose model, and 6.31–81.55 and 1.06–74.45 by MMP-1 inhibitory activity, respectively.
Conclusion: Leaves and bark extracts of GP (Retz.) DC. are good sources of important phytonutrients and have the potential in anti-aging property.
Integr Med 2014;11:151-69.
2. Choi YH, Seo C, Jeong W, Lee JE, Lee JY, Ahn EK, et al.
Glycopentanolones A-D, four new geranylated quinolone alkaloids
from Glycosmis pentaphylla. Bioorg Chem 2019;87:714-9.
3. Howlader A, Rizwan F, Sultana S, Rahman MR, Shams-Ud-Doha KM,
Mowla R, et al. Antimicrobial, antioxidant and cytotoxic effects of
methanolic extracts of leaves and stems of Glycosmis pentaphylla
(Retz.). J Appl Pharm Sci 2011;1:137-40.
4. Sreejith PS, Praseeja RJ, Asha VV. A review on the pharmacology and
phytochemistry of traditional medicinal plant, Glycosmis pentaphylla
(Retz.) Correa. J Pharm Res 2012;5:2723-8.
5. Wagner KH, Cameron-Smith D, Wessner B, Franzke B. Biomarkers of
aging: From function to molecular biology. Nutrients 2016;8:338-50.
6. Gonzalo-Calvoa D, Neitzert K, Fernández M, Vega-Naredo I,
Caballero B, García-Macía M, et al. Differential inflammatory
responses in aging and disease: TNF-? and IL-6 as possible biomarkers.
Free Radic Biol Med 2010;49:733-7.
7. Zhaoa L, Yanga H, Xuc M, Wanga X, Wanga C, Lianc Y, et al.
Stevia residue extract ameliorates oxidative stress in D-galactoseinduced
T aging mice via Akt/Nrf2/HO-1 pathway. J Funct Foods
8. Sharma N. Free radicals, antioxidants and disease. Biol Med 2014;6:3.
9. Shalaby EA, Shanab SM. Comparision of DPPH and ABTS assays for
determining antioxidant potential of water and methanol extracts of
Spirulina platensis. Indian J Geo Mar Sci 2013;42:556-64.
10. Chen X, Li B, Xie H, He Y, Zhong D, Chen D. Antioxidant structure
activity relationship analysis of five dihydrochalcones. Molecules
11. Lipinski B. Hydroxyl radical and its scavengers in health and disease.
Oxid Med Cell Longev 2011;2011:809696.
12. Li X. Solvent effects and improvements in the deoxyribose degradation
assay for hydroxyl radical-scavenging. Food Chem 2013;141:2083-8.
13. Ca?yniuk B, Grochowska-Niedworok E, Walkiewicz KW, Kawecka S,
Popio?ek E. Malondialdehyde (MDA) product of lipid peroxidation
as marker of homeostasis disorders and aging. Ann Acad Med Sci
14. Alam MM, Ahmad I, Naseem I. Inhibitory effect of quercetin in the
formation of advanced glycation end products of human serum albumin:
An in vitro and molecular interaction study. Int J Biol Macromol
15. Zhang L, Zhang CJ, Tu ZC, Yang WH, Zhao Y, Xin ZQ, et al. Nelumbo
nucifera leaf extracts inhibit the formation of advanced glycation endproducts
and mechanism revealed by Nano LC-Orbitrap-MS/MS. J
Funct Foods 2018;42:254-61.
16. Vinson JA, Howard TB 3rd. Inhibition of protein glycation and
advanced glycation end products by ascorbic acid and other vitamins
and nutrients. J Nutr Biochem 1996;7:659-63.
17. Kuda T, Eda M, Kataoka M, Nomoto M, Kawahara M, Oshio S,
et al. Anti-glycation properties of the aqueous extract solutions of dried
algaeproducts and effect of lactic acid fermentation on the properties.
Food Chem 2016;192:1109-15.
18. Sutinen M, Kainulainen T, Hurskainen T, Vesterlund E, Alexander JP,
Overall CM, et al. Expression of metrix metalloproteinases (MMP-1
and -2) and their inhibitors (TIMP-1, -2 and -3) in oral lichen planus,
dysplasia, squamous cell carcinoma and lymph node metastasis. Br J
19. Azumi N, Hashim P, Hashim DM, Halimoon N, Majid NM. Antielastase,
anti-tyrosinase and matrix metalloproteinase-1 inhibitory
activity of earthworm extracts as potential new anti-aging agent. Asian
Pac J Trop Biomed 2014;4:348-52.
20. Cho S, Kim HH, Lee MJ, Lee S, Park CS, Nam SJ, et al.
Phosphatidylserine prevent UV-induced decrease if type I collagen and
incease of MMP-1 in dermal fibroblasts and human skin in vivo. J Lipid