THEVETIA PERUVIANA ROOTS EXTRACT MEDICATED GOLD NANOPARTICLES AND ITS UREASE INHIBITORY ACTIVITY
Objective: The main objective of the current research work was synthesized of gold nanoparticles (AuNPs) of Thevetia peruviana aqueous extract,
characterization, and screening for urease enzyme inhibitory activity.
Methods: AuNPs were synthesized by mixing 1 mM gold salt solution with T. peruviana aqueous extract without any reducing agents. The prepared
AuNPs were characterized using UV–visible spectroscopy, Fourier transform infrared spectroscopy, and scanning electron microscopy techniques. The
synthesized AuNPs were assessed for in vitro urease enzyme inhibitory activity at 0.2 μg in comparison with the aqueous extract.
Results: In this finding, we synthesized the AuNPs of T. peruviana aqueous extract for the 1st time. The AuNPs exhibited significant stability at
room temperature. The AuNPs showed significant urease inhibitory activity with IC50 67.56±1.67 at 0.2 μg as compared to aqueous extract which
exhibited good activity with IC50 39.21±1.32 μ at 0.2 mg, against standard thiourea (IC50=21.00±1.16). The formation of AuNPs correlates due to active
phytochemical present in extract which is responsible for synthesizing NPs.
Conclusion: T. peruviana extract and prepared AuNPs are an outstanding urease enzyme inhibitor and are capable of making fine NPs. Application:
The synthesized AuNPs of T. peruviana aqueous extract which significant urease inhibitory activity may allow us to discover NPs for potentially
effective and safe nanoherbal therapy.
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