STRUCTURAL DETERMINATION OF L-ASPARAGINASE II OF STREPTOMYCES ALBIDOFLAVUS AND INTERACTION ANALYSIS WITH L-ASPARAGINE AND CEFOTAXIME

  • ACHYUTUNI VENKATA NAGA TEJASWINI School of Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kakinada, 533003, Andhra Pradesh, India
  • MALOTHU RAMESH Associate Professor and Head, School of Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kakinada, 533003, Andhra Pradesh, India

Abstract

Objective: L-Asparaginase enzyme possesses a crucial role in treatment of various hematological malignancies. The current study focuses on homology modeling and interaction analysis of L-Asparaginase proteins belonging to Streptomyces albidoflavus (S. albidoflavus) with the essential ligand L-Asparagine and subsequent analysis with essential β-lactam antibiotic Cefotaxime. 


Methods: The process of understanding of Asparaginase interactions primarily involved structure determination of WP_096097608, WP_095730301, which is achieved by GalaxyTBM, I-TASSER and SWISS MODEL. Further the S. albidoflavus Asparaginase proteins are subjected to GalaxySite and Autodock Vina of PyRx analysis.


Results: The GalaxyTBM predicted structures of both the proteins are found promising on various validation studies.  The two Asparaginase proteins exhibited high binding affinities of     -6.8 and -6.5 kcal/mol with Cefotaxime and -5.1 and -4.9 kcal/mol towards Asparagine. The protein WP_096097608 residues forming hydrogen bonds with L-Asparagine are also analysed to involve in interaction with Cefotaxime on individual docking analysis.  


Conclusion: The current findings details the two S. albidoflavus Asparaginase proteins affinity towards L-Asparagine, hence can be assessed further for immunogenicity studies. In addition to the above findings, an attempt is made to find the L-Asparaginase binding possibilities with non-metals, that identified an essential β-lactam antibiotic Cefotaxime to be an effective inhibitor. This study helps in understanding the interactions of L-Asparaginase with Cefotaxime, as intake of antibiotics between the phases of chemotherapy is observed to treat various infections and also as an antibiotic to microbes which utilize Asparaginase as a vital enzyme. 

Keywords: L-Asparaginase, Streptomyces, Homology modelling, GalaxyTBM, Cefotaxime, L-Asparagine
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TEJASWINI, A. V. N., & RAMESH, M. (2021). STRUCTURAL DETERMINATION OF L-ASPARAGINASE II OF STREPTOMYCES ALBIDOFLAVUS AND INTERACTION ANALYSIS WITH L-ASPARAGINE AND CEFOTAXIME. International Journal of Applied Pharmaceutics, 13(4). https://doi.org/10.22159/ijap.2021v13i4.41303
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