TOPOISOMERASE INHIBITORS ACTIVITY OF YELLOW CHAMPAKA (MICHELIA CHAMPACA L.) BARK EXTRACTS AND FRACTIONS AND ITS LIRIODENINE CONTENTS
Keywords:Yellow champaka, Michelia champaca L., Topoisomerase inhibitors, Mechanism-based yeast bioassay
Objective: This research is aimed to investigate the activity of various extracts and fractions of yellow champaka (Michelia champaca L.) by mechanism-based yeast bioassay (MBYB) against the mutant yeasts. Liriodenin content, an active compound in M. champaca, will analyze to know the correlation with this assay.
Methods: The bark of M. champaca was extracted by maceration and graded maceration using n-hexane, ethyl acetate, and methanol. The methanol extract was fractionated using water, n-hexane and ethyl acetate in the liquid-liquid extraction process. Each extract and fractions were tested in vitro by mechanism-based yeast bioassay for their topoisomerase inhibitor activity. Liriodenine content of all samples was analyzed by thin layer chromatography (TLC) densitometry method.
Results: The yeast bioassay results showed that all extracts were active as topoisomerase inhibitors (IC12 values under 8000 µg/ml) except MGM. Liriodenine can be used as a marker of active compound in ethyl acetate samples that were EAM, EAGM and EAF with Pearson analysis value-0.887 (P=0.153) and these samples were relatively more active than others.
Conclusion: This research showed that various extracts and fractions of yellow champaka bark have topoisomerase inhibitor's activity. Liriodenine can be used as a marker of the active compound so yellow champak bark is a potent natural agents for breast cancer.
Forman D, Ferlay J. The global and regional burden of cancer. In: Stewart BW, Wild CP, editors. World cancer report. Geneva: WHO Press; 2014. p. 16-53.
International Agency for Research on Cancer (IARC). Fact Sheet IARC: 2019.
Ministry of Health Republic of Indonesia. Main research of national basic of Health Research 2018. The agency of Research and Development. Jakarta: Ministry of Health RI; 2019.
Ministry of Health Republic of Indonesia, Stop Cancer. Center of Data and Information, Jakarta: Ministry of Health RI; 2015.
Lyon L. Diagnosis and treatment of cancer. In: Bozzone DM. editor. The biology of cancers. New York: Chelsea house Publishers; 2007. p. 11-131.
National Cancer Institute (NCI). A to Z list of anticancer drugs. Available from: https://www.cancer.gov/about-cancer/ treatment/drugs. [Last accessed on 10 Oct 2016]
The Indonesian Pharmacists Association. Information of Indonesian drug Spesialite Volume 50. Antineoplastic, Immunosuppressant and drug for Paliatic. Jakarta: PT ISFI publisher; 2016.
Hoffmann JJ, Torrance SJ, Widehopf RM, Cole JR. Cytotoxic agents from Michelia champaca and Talauma ovata: parthenolide and costunolide. J Pharm Sci. 1977;66(6):883-4. doi: 10.1002/jps.2600660642, PMID 889597.
Ananthi T, Chitra M, Aruna B. In vitro anticancer activity of Michelia champaca L. flowers against Ehrlich ascites carcinoma cell line. Int J Pharm Biol Sci. 2014;5(4):357-63.
Zuhrotun A, Suganda AG, Wirasutisna KR, Wibowo MS. Anticancer screening of selected apocynaceae, simaroubaceae and magnoliaceae of Indonesian plants using mechanism-based yeast bioassay. Int J Pharm Sci Rev Res. 2015;35(2):90-4.
Zuhrotun A, Suganda AG, Wirasutisna KR, Wibowo MS. Isolation of the bioactive compound of michelia champaca L. Bark and its activity test using mechanism-based yeast bioassay. Asian J Pharm Clin Res. 2016;9(5):158-61. doi: 10.22159/ajpcr.2016.v9i5.12856.
Mongkolrat S, Palanuvej C, Ruangrungsi N. Thin layer chromatography and image analysis of selected liriodenine bearing plants in Thailand. J Health Res. 2013;27(2):67-72.
Gunatilaka AAL, Kingston DGI. DNA-damaging natural product with potential anticancer activity. In: Atta-ur-Rahman. editor. Studies in natural products chemistry: structure and chemistry (part F). Amsterdam: Elsevier Science BV; 1998. p. 457-505.
Mukaka MM. Statistics Corner: A guide to the appropriate use of correlation coefficient in medical research. Malawi Med J. 2012;24(3):69-71. PMID 23638278.
Cragg GM, Kingston DGI, Newman DJ. Anticancer agents from natural products. Camptothecin and its Analogs. Boca Raton: CRC Press. 2005.
Yeh YT, Huang JC, Kuo PL, Chen CY. Bioactive Constituents from Michelia champaca. Nat Prod Commun. 2011;6(9):1251-2. PMID 21941890.
Zhu Z, Shen W, Tian S, Yang B, Zhao H. F3, a novel active fraction of Valeriana jatamansi Jones induces cell death via DNA damage in human breast cancer cells. Phytomedicine. 2019;57:245-54. doi: 10.1016/j.phymed.2018.12.041, PMID 30797986.
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