QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL ANALYSIS AND BACTERICIDAL ACTIVITY OF PELARGONIUM GRAVEOLENS L’HER


M. Pradeepa, V. Kalidas, N. Geetha

Abstract


  1. Objective: The present study investigates the qualitative and quantitative phytoconstituents and bactericidal effect of medicinally important Pelargonium graveolens L’ Her leaves extracts.

    Methods: Preliminary phytochemical screening analyses were determined using standard protocol methods. In addition, antibacterial activities of the P. graveolens leaves extracts were evaluated by disc diffusion method against Gram-positive and Gram-negative bacteria.

    Results: The results revealed that ethanolic extract of P. graveolens was found to be the predominant occurrence of phytochemicals (9/11) which contains flavonoid, phenol, tannins, saponins, reducing sugar, glycosides, terpenoids, anthraquinone, and phlorotannins while starch and steroids were absent. In quantitative estimation of bioactive phytoconstituents showed carbohydrates (74 mg/gdw), protein (41.25 mg/gdw), chlorophyll (2.2±0.05 mg/gdw), lipids (0.07 mg/gdw), tannins (135.3 gm/gdw), phenolic compounds (123.75 mg/gdw) and flavonoids (50 mg/gdw) were found to have higher amount in ethanolic extract followed by acetone, methanol and aqueous extract and also showed an inhibitory action on growth of tested bacteria.

    Conclusion: Ethanolic extract of the P. graveolens leaves hold promises as a potential source of pharmaceutically important phytochemicals and also have strong antibacterial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa and Klebsiella pneumonia.

    Keywords: Phytochemical screening, Primary and secondary metabolites, Antibacterial screening, Pelargonium graveolens

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References


Okwu DE. Flavoring properties of spices on cassava fufu. Afr J Root Tuber Crops 1999;3:19-21.

Pradeepa M, Kalidas V, Geetha N. An In silico approach for the identification of anti-colon cancer phytocompounds from Avena sativa. L. Int J Pharm Biol Sci 2014;5:543–52.

Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, Ghaffar R, et al. Phytochemical analysis of medicinal plants occurring in local area of mardan. Biochem Anal Biochem 2013;2:1-4.

Linga rao M, Savithramma N. Quantification of primary and secondary metabolites of Svensonia hyderobadensis–a rare medicinal plant. Int J Pharm Pharm Sci 2012;4:519-21.

Loizzo MR, Tundis R, Conforti F, Saab AM, Statti GA, Menichini F. Composition and α-amylase inhibitory effect of essential oils from Cedrus libani, Fitoterapia. 2007;78:323–6.

Senthilkumar M. Phytochemical screening of Gloriosa superba L.-from different geographical position. Int J Sci Res Publications 2013;3:1-5.

Sagwan S, Rao DV, Sharma RA. Biochemical estimation of primary metabolites from Pongamia Pinnata (L.): an important biodiesel plant. Int J Pharm Sci Rev Res 2010;5:146-9.

Arvind J Mungole, Ravi Awati, Alka Chaturvedi, Prakash Zanwar. Preliminary Phytochemical screening of ipomoea obscura (L)-A hepatoprotective medicinal plant, Int J PharmTech Res 2010;2:2307-12.

Derwich E, Benziane Z, Boukir A. Chemical composition of leaf essential oil of Juniperus phoenicea and evaluation of its antibacterial activity, Int J Agric Biol 2010;12:199-204.

Saraswathi J, Venkatesh K, Baburao N, Hilal MH, Roja Rani A. Phytopharmacological importance of pelargonium species. J Med Plants Res 2011;5:2587–98.

Saxena G, Laiq-ur-Rahman, Praveen Chandra Verma, Suchitra Banerjee, Sushil Kumar. Field performance of some clones of rose scented geranium (Pelargonium graveolens L’Her Ex Ait.) for evaluation of their essential oil yield and composition. Ind Crops Prod 2008;27:86–90.

Arumugam R, Kumar N. Geranium cultivation in Kodaikanal Hills. Indian Perfumer 1979;23:128-30.

Lalli JYY, Van Zyl RL, Van Vuuren SF, Viljoen AM. In vitro biological activities of South African pelargonium (Geraniaceae) species. S Afr J Bot 2008;74:153-7.

Monika B, Małgorzata W, Danuta K, Monika S. Antimicrobial activity of geranium oil against clinical strains of Staphylococcus aureus. Mol 2012;17:10276-91.

Naeini AR, Nazeri M, Shokri H. Antifungal activity of Zataria multiflora, Pelargonium graveolens and Cuminum cyminum essential oils towards three species of Malassezia isolated from patients with Pityriasis versicolor. J Med Vet Mycol 2011;21:87-91.

Maruyama N, Ishibashi H, Hu W, Morofuji S, Inouye S, Yamaguchi H, et al. Suppression of carrageenan and collagen II-induced inflammation in mice by geranium oil. Mediators Inflammation 2006;53:625-37.

Boukhris M, Bouaziz M, Feki I, Jemai HE, Feki A, Sayadi S. Hypoglycemic and antioxidant effects of leaf essential oil of pelargonium graveolens L’Hér. in alloxan induced diabetic rats. Lipids Health Dis 2012;11:1-10.

Galea C, Hancu G. Antimicrobial and antifungal activity of Pelargonium roseum essential oils. Adv Pharm Bull 2014;4:1-4.

Kader G, Nikkon F, Rashid MA, Yeasmin T. Antimicrobial activities of the rhizome extract of Zingiber zerumbet Linn. Asian Pac J Trop Biomed 2011;1:409-12.

Kavit M, Pate BN, Jain BK. Phytochemical analysis of leaf extract of Phyllanthus fraternus. Res J Recent Sci 2013;2:12-5.

Ugochukwu SC, Uche IA, Ifeanyi O. Preliminary phytochemical screening of different solvent extracts of stem bark and roots of Dennetia tripetala G. Baker. Asian J Plant Sci Res 2013;3:10-3.

Iqbal PJ. Phytochemical screening of certain plant species of Agra city. J Drug Delivery Ther 2012;2:135-8.

Agarwal K, Varma R. Studies on antioxidant activity and phytochemical screening of selected medicinal plant ocimum gratissimum L. Int J Pharm Sci Res 2014;5:3520-8.

Hedge JE, Hofreiter BT, Whistler RL, Be Miller JN. In: Carbohydrate Chemistry. 17th Ed. Academic Press: New York; 1962.

Lowery OH, Rosenberg NJ, Farr AL, Randal RJ. Protein measurement with the folin-phenol reagent. J Biol Chem 1951;193:265-75.

Geetha TS, Geetha N. Phytochemical screening, quantitative analysis of primary and secondary metabolites of cymbopogan citratus (DC) stapf. leaves from Kodaikanal hills, Tamilnadu. Int J PharmTech Res 2014;6:521-9.

Folch J, Lees M, Standly GHS. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 1957;226:497-509.

McDonald S, Prenzer PD, Autolovich M, Robards K. Phenolic content and antioxidant activity of olive extracts. Food Chem 2001;73:73-84.

Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.

Schanderl S. In: Methods in food analysis. Acedemic Press: in New York; 1970. p. 70.

Singh R, Muftah AMS, Belkheir A. Antibacterial and antioxidant activities of Mentha piperita L. Arabian J Chem 2015;8:322-8.

Tanwer BS, Vijayvergia R. Phytochemical Evaluation and Quantification of Primary metabolites of Alangium salviifolium. Int J Pharm Biol Sci 2010;1:1-6.

Ambasta SP, Ramachandran K, Kashyapa K, Chand R. Useful plants of India. Publication and information directorate. Council of Scientific and Industrial Research. New Delhi; 1986. p. 433–7.

Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga Latha L. Extraction, Isolation and characterization of Bioactive compounds from Plants extracts. Afr J Tradit Complementary Altern Med 2011;8:1-10.

Kiruba S, Mahesh M, Nisha SR, Miller Paul Z, Jeeva S. Phytochemical analysis of the flower extracts of Rhododendron arboretum Sm. ssp. nilagiricum (Zenker) Tagg. Asian Pac J Trop Biomed 2011;1:284-6.

Arya V, Thakur N, Kashyap CP. Preliminary Phytochemical Analysis of the extracts of Psidium Leaves. J Pharmacogn Phytochem 2012;1:1-5.

Ghongade R. Phytochemical analysis of citrus karna fruit. Int J Pharm Biol Sci 2013;4:(B)1162–7.

Sasikala A, Linga Rao M, Savithrama N. Quantification of primary and secondary metabolites from leaves and stem bark of Cochlospermum religiosum (L) Alston. Int Res J Pharm 2013;4:228-31.

Govaerts R. How many species of seed plants are there. Taxon 2001;50:1085–90.

Ulhe SK, Narkhede SD. Histological and phytochemical studies on aromatic plant, Hyptis suaveolens (L.) of family Lamiaceaea (MS). India Sci Res Reporte 2013;3:44-8.

Geidam YA, Ambali AG, Onyeyli PA. Preliminary phytochemical and antibacterial evaluation of crude aqueous extracts of Psidium guajava L. leaf. J Appl Sci 2007;7:511-4.

Sharma BL, Daulat Singh, Santosh Sharma K, Afzal Hashmi, Arjun Singh, Anil Bansal. Studies on some primary metabolite’s extraction and Quantification in different plant parts of selected Cassia Species. Asian J Pharm Clin Res 2013;6:309-14.

Lee JH, Park KH, Lee MH, Kim HT, Seo WD, Kim WD, et al. Identification, characterization, and quantification of phenolic compounds in the antioxidant activity-containing fraction from the seeds of Korean perilla (Perilla frutescens) cultivars. Food Chem 2013;136:843-53.

Gayathri P, Gayathri Devi S, Sivagami S, Saroja S. Screening and quantitation of phytochemicals and nutritional components of the fruit and bark of Helicteres isora. Hygeia: J Drugs Med 2010;2:57-62.

Essiett UA, Edet NI, Bala DN. Phytochemical and physicochemical analysis of the leaves of Laportea aestuans (Linn.) Chew and Laportea ovalifolia (Schumach.) chew (male and female). Asian J Plant Sci Res 2011;1:35-42.

Hsouna AB, Hamdi N. Phytochemical composition and antimicrobial activities of the essential oils and organic extracts from Pelargonium graveolens growing in Tunisia. Lipids Health Dis 2012;11:167.




About this article

Title

QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL ANALYSIS AND BACTERICIDAL ACTIVITY OF PELARGONIUM GRAVEOLENS L’HER

Date

03-05-2016

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 8, Issue 3, 2016 Page: 7-11

Online ISSN

0975-7058

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278 Views | 153 Downloads

Authors & Affiliations

M. Pradeepa

V. Kalidas

N. Geetha
Department of Biotechnology, Mother Teresa Women’s University, Kodaikanal 624101, India


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