@article{Maraie_Almajidi_Alshadher_2018, title={ABSOLUTE AND RELATIVE BIOAVAILABILITY STUDY FOR THE NEWLY DEVELOPED NASAL NANOEMULSION IN SITU GEL OF ONDANSETRON HCl IN COMPARISON TO CONVENTIONALLY PREPARED IN SITU GEL AND INTRAVENOUS DOSAGES FORMS}, volume={10}, url={https://journals.innovareacademics.in/index.php/ijap/article/view/27396}, DOI={10.22159/ijap.2018v10i5.27396}, abstractNote={<p><strong>Objective: </strong>The aim of the work was to study the absolute and relative bioavailability (using rabbits) of <em>ondansetron HCl</em> (ONH)from our newly prepared intranasal mucoadhesive nanoemulsion <em>in situ</em> gel (NIG) in comparison to intranasal mucoadhesive in situ gel (IG) prepared by the conventional method and intravenous injection.</p><p><strong>Methods: </strong>Six male rabbits weighing 2.5-3 kg were used in this study, where the dose of <em>ondansetron HCl</em> (ONH) was calculated based on the body surface area (BSA) which is equivalent to 140μl (containing 10 mg/ml) of NIG and IG and 700μl of intravenous Zofran<sup>® </sup>injection (containing 2 mg/ml) were given to the rabbits, separated with one week washout period. Serial blood samples were withdrawn and analyzed for simultaneous determination of the drug using HPLC (Knaure; 150 ×4.6 mm; 5 μm particle size; 25 cm length) supported by guard column C18-4 mm diameter.<strong></strong></p><p class="Default"><strong>Results: </strong>The pharmacokinetics parameters for NIG; Cmax, Tmax, AUC0-t, AUC0-∞were found to be greater than conventional in situ gel (IG). <em>In vivo</em> pharmacokinetic studies in rabbits showed a significant increase in C<sub>max</sub> and AUC <sub>0-α</sub>(P<0.001) with shorter T<sub>max</sub>using NIG compared to IG containing the same NIG excipients, while the absolute bioavailability for NIG and IG (was 80.541 and 51.068 respectively).</p><p><strong>Conclusion: </strong>The present studies ratify the bioavailability enhancement potential of NE used to prepare NIG for the drug and significantly high absolute bioavailability to be used as a successful alternative route to the IV injection and improve patient compliance.</p>}, number={5}, journal={International Journal of Applied Pharmaceutics}, author={Maraie, Nidhal K. and Almajidi, Yasser Q. and Alshadher, Ahmed}, year={2018}, month={Sep.}, pages={105–109} }