@article{Harahap_Suwanto_._2018, title={INCURRED SAMPLE STABILITY OF METFORMIN HYDROCHLORIDE IN PLASMA OF SIX HEALTHY SUBJECTS USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY}, volume={10}, url={https://journals.innovareacademics.in/index.php/ijap/article/view/31509}, DOI={10.22159/ijap.2018.v10s1.58}, abstractNote={<p><strong>Objective</strong>: This study aimed to measure metformin levels and evaluates the incurred stability in plasma of six healthy subjects after administration<br />850 mg metformin hydrochloride tablet using high-performance liquid chromatography.<br /><strong>Methods</strong>: Samples were collected from six healthy subjects who were administered 850 mg metformin hydrochloride tablet. Blood was collected from<br />the subjects at up to 12-time points throughout a 12 h period. Sample stability was analyzed on days 7, 14, and 30. The analysis was conducted using<br />C-18 columns, a column temperature of 40°C, a mobile phase consisting of acetonitrile-phosphate buffer (pH 7.0; 40:60 v/v), a flow rate of 1 mL/min,<br />the photodiode array detector set at a wavelength of 234 nm, and calcium atorvastatin as the internal standard.<br /><strong>Results</strong>: All metformin HCl samples collected on days 7, 14, and 30 from subjects 1 to 6 met stability requirement based on the European Medicines<br />Agency Bioanalytical Guideline (2011), which state that the percentage difference value not >20%. In addition, the developed method for analyzing<br />metformin HCl in plasma was linear in the concentration range of 20.0–5000.0 ng/mL (r=0.9999).<br /><strong>Conclusion</strong>: The incurred sample of metformin hydrochloride in plasma of six healthy subjects was stable until 30 days after drug administration.</p>}, number={1}, journal={International Journal of Applied Pharmaceutics}, author={Harahap, Yahdiana and Suwanto, Ferry Ivan Balto and ., Sunarsih}, year={2018}, month={Dec.}, pages={260–263} }