@article{Ekasary_._Maggadani_2018, title={OPTIMIZED HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-FLUORESCENCE DETECTION METHOD FOR THE MEASUREMENT OF GLYCINE, PROLINE, AND HYDROXYPROLINE CONCENTRATIONS IN PORCINE GELATIN}, volume={10}, url={https://journals.innovareacademics.in/index.php/ijap/article/view/31533}, DOI={10.22159/ijap.2018.v10s1.72}, abstractNote={<p><strong>Objective</strong>: The aim of this study is to develop an optimized method for glycine, proline, and hydroxyproline content quantitation in porcine skin<br />gelatin.<br /><strong>Methods</strong>: Gelatin was isolated from porcine skin by hydrolysis for 24 h in 0.5 M acetic acid, heating in distilled water at 55°C for 3 h, and drying at<br />60°C. The extract was evaluated by organoleptic tests, Fourier-transform infrared spectroscopy, moisture assay, ash assay, and viscosity test. Gelatin<br />amino acids were derivatized using 9-fluorenylmethylchloroformate-chloride and measured by high-performance liquid chromatography (HPLC)<br />with fluorescence detection using a C18 column after the optimization of the mobile phase composition, flow rate, and detection wavelengths.<br /><strong>Results</strong>: The optimized parameters for the quantitation of glycine, proline, and hydroxyproline by HPLC with fluorescence detection were as<br />follows: Excitation wavelength, 265 nm; emission wavelength, 320 nm; mobile phase composition acetic buffer: acetonitrile, 55:45; and flow<br />rate, 0.8 mL/min. The average proportional amino acid contents were 28.57±0.74%, 19.24±0.48%, and 2.89±0.33% for glycine, proline, and<br />hydroxyproline, respectively.<br /><strong>Conclusion</strong>: This method allows for sensitive and accurate quantitation of glycine, proline, and hydroxyproline in porcine skin gelatin samples for<br />quality control and source determination.</p>}, number={1}, journal={International Journal of Applied Pharmaceutics}, author={Ekasary, Aryaty and ., Harmita and Maggadani, Baitha Palanggatan}, year={2018}, month={Dec.}, pages={352–330} }