@article{Kurniawan_Sugiarti_Wanandi_Louisa_2018, title={PIPERINE IN THE PREVENTION OF THE DECREASED TAMOXIFEN SENSITIVITY IN MCF-7 BREAST CANCER CELL LINE}, volume={10}, url={https://journals.innovareacademics.in/index.php/ijap/article/view/31536}, DOI={10.22159/ijap.2018.v10s1.74}, abstractNote={<p><strong>Objective</strong>: This study was designed to analyze the role of piperine in modulating P-glycoprotein mRNA expression when added in combination with<br />tamoxifen to breast cancer cells in culture.<br /><strong>Methods</strong>: MCF-7 breast cancer cells were treated with 1 μM tamoxifen with or without piperine (12.5, 25, or 50 μM) or verapamil 50 μM (P-glycoprotein<br />inhibitor positive control) for up to 12 days. We assessed the cell viability and isolated total RNA from them. We quantified P-glycoprotein expressions<br />using quantitative reverse transcription polymerase chain reaction.<br /><strong>Results</strong>: Administration of various doses of piperine decreased MCF-7 breast cancer cell viability. Piperine, when given in combination with tamoxifen,<br />decreased the expression of P-glycoprotein mRNA in cells compared with the expression in cells treated with tamoxifen only. The effects were shown<br />to be dose dependent.<br /><strong>Conclusion</strong>: Piperine prevents the development of breast cancer cell tamoxifen resistance, probably through its inhibition of P-glycoprotein expression.</p>}, number={1}, journal={International Journal of Applied Pharmaceutics}, author={Kurniawan, Sandy Vitria and Sugiarti, Lies and Wanandi, Septelia Inawati and Louisa, Melva}, year={2018}, month={Dec.}, pages={335–337} }