@article{Harahap_Astrini_Suryadi_2018, title={VALIDATION OF 6-MERCAPTOPURINE AND 6-THIOGUANINE IN DRIED BLOOD SPOT BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY}, volume={10}, url={https://journals.innovareacademics.in/index.php/ijap/article/view/31579}, DOI={10.22159/ijap.2018.v10s1.91}, abstractNote={<p><strong>Objective</strong>: This study aimed to obtain an optimal and validated method of analyzing 6-mercaptopurine (6-MP) and 6-thioguanine (6-TG) simultaneously<br />in dried blood spot samples using ultra high-performance liquid chromatography-tandem mass spectrometry.<br /><strong>Method</strong>: Separation was performed with a 1.7-μm amide column, which had a mobile phase with a flow rate of 0.2 mL/min and comprised 0.2%<br />formic acid in water, 0.1% formic acid in acetonitrile, and methanol with a gradient elution. Detection was performed using Waters Xevo TQD.<br /><strong>Result</strong>: This method was linear with a range of 25–1000 ng/mL for 6-MP and 6-TG, with consecutive r values of ≥0.996 and ≥0.995, respectively. The<br />intra- and inter-day % difference value and coefficient of variation for the accuracy and precision were not more than 15% and 20%, respectively, at<br />a concentration lower limit of quantitation.<br /><strong>Conclusion</strong>: This method fulfilled the requirements of the European Medicines Agency guideline for validation.</p>}, number={1}, journal={International Journal of Applied Pharmaceutics}, author={Harahap, Yahdiana and Astrini, Cheputri Rahma and Suryadi, Herman}, year={2018}, month={Dec.}, pages={412–415} }