@article{CARABELLY_ASPRIYANTO_APRIASARI_RIZKY_2019, title={THE TOXICITY OF MAULI BANANA (MUSA ACUMINATA) STEM WATER EXTRACT ON BONE MARROW MESENCHYMAL STEM CELL IN VITRO}, volume={11}, url={https://journals.innovareacademics.in/index.php/ijap/article/view/33428}, DOI={10.22159/ijap.2019.v11s1.16762}, abstractNote={<p><strong>Objective</strong>: Since mesenchymal stem cells (MSC) can differentiate into bone, cementum, and periodontal ligament, they can be used to treat aggressive<br>periodontitis. The limited number of MSCs requires replenishment of growth factor in the cell culture process. Since growth factor is quite expensive,<br>an alternative material is needed. Mauli banana stem has antioxidant and immunomodulatory properties. Methanol extract of Mauli banana stem is<br>known to be toxic toward MSCs; therefore, another solvent with a non-toxic effect is needed, such as a water solvent. We analyzed the toxicity of Mauli<br>banana stem water extract on MSC in vitro.<br><strong>Methods</strong>: In this laboratory experimental (true experimental) study with a Post-test Only Control Group Design, MSC cultures were treated with<br>Mauli banana stem water extract at 10, 20, 40, 60, 80, and 100 mg/mL dosages. One group without any treatment served as a control group and one<br>was a media control group. Each group was incubated for 24 h and then was given 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide<br>reagent and analyzed by an enzyme-linked immunosorbent assay (ELISA) reader.<br><strong>Results</strong>: One-way analysis of variance showed a significant difference.<br><strong>Conclusion</strong>: Mauli banana stem water extracts at 10, 20, 40, and 60 mg/mL were not toxic toward MSC in vitro, while dosages of 80 and 100 mg/mL<br>dosage were toxic.</p>}, number={1}, journal={International Journal of Applied Pharmaceutics}, author={CARABELLY, AMY NINDIA and ASPRIYANTO, DIDIT and APRIASARI, MAHARANI LAILLYZA and RIZKY, SELVIANA}, year={2019}, month={Apr.}, pages={184–186} }