@article{GARG_AGRAWAL_K._2019, title={DEVELOPMENT AND VALIDATION OF REVERSED PHASE HPLC-PDA METHOD FOR THE QUANTIFICATION OF CHRYSIN IN SOLID LIPID NANOPARTICLES}, volume={11}, url={https://journals.innovareacademics.in/index.php/ijap/article/view/33904}, DOI={10.22159/ijap.2019v11i5.33904}, abstractNote={<p><strong>Objective: </strong>The main aim of the present study was to develop and validate a simple, precise and accurate Reversed-Phase HPLC-PDA method for the quantitative determination of Chrysin in solid lipid nanoparticles (SLNs).</p> <p><strong>Methods: </strong>The RP-HPLC-PDA system equipped with a C-18 reversed-phase column (250 × 4.6 mm, particle size 5 μm) was employed in the present study. HPLC grade methanol and water in 85:15 (v/v) ratio was selected as the mobile phase at flow rate of 1 ml/min under an ambient column oven temperature. The detection wavelength was kept at 268 nm. Validation of developed method was performed according to the ICH guidelines.</p> <p><strong>Results: </strong>The developed reversed-phase HPLC-PDA method was found to be linear in the concentration range of 0.2-10 µg/ml with a correlation coefficient of 0.999. The method was also observed to be precise with % relative standard deviation (RSD) below 2%. The limit of detection and limit of quantification of this method were found to be 0.05µg/ml and 0.14µg/ml, respectively. The percent recovery of the developed method was estimated to more than 99%.</p> <p><strong>Conclusion: </strong>The developed HPLC method can be utilized for the determination of Chrysin with a high degree of accuracy, precision, robustness, specificity in solid lipid nanoparticles in the presence of excipients.</p>}, number={5}, journal={International Journal of Applied Pharmaceutics}, author={GARG, ANUJ and AGRAWAL, GOPAL PRASAD and K., SHAHADALI}, year={2019}, month={Sep.}, pages={324–328} }