@article{SEIFU_NATH_DUTTA_2020, title={HYALURONIC ACID-DOCETAXEL CONJUGATE LOADED NANOLIPOSOMES FOR TARGETING TUMOR CELLS}, volume={12}, url={https://journals.innovareacademics.in/index.php/ijap/article/view/39026}, DOI={10.22159/ijap.2020v12i6.39026}, abstractNote={<p><strong>Objective: </strong>Docetaxel (DTX), a potent anticancer drug, is suffering from non-specificity and drug resistance as major limitations. In this investigation, we developed Hyaluronic acid (HA)-Docetaxel conjugate (HA-DTX) loaded nanoliposomes to target cancer cells via passive and active targeting approaches.</p> <p><strong>Methods: </strong>HA-DTX was synthesized and characterized by UV-Visible spectrophotometry, FT-IR spectroscopy, <sup>1</sup>H NMR spectroscopy, Differential scanning calorimetry and X-ray diffraction and then loaded into nanoliposomes (L-NLs) by thin-film hydration method. L-NLs were characterized physicochemically and evaluated for anticancer efficacy by <em>in vitro</em> cytotoxicity study in glioma cells (C6 glial cells); cellular uptake and apoptotic effect were investigated by fluorescence microscopy.</p> <p><strong>Results: </strong>HA-DTX was successfully synthesized; L-NLs had an average size of 123.0<strong>±</strong>16.53 nm, polydispersity index of 0.246<strong>±</strong>0.01 and zeta potential of -44.4<strong>±</strong>6.79 mV. Also, L-NLs exhibited 90.54%<strong>±</strong>4.22 of drug loading efficiency and 2.68%<strong>±</strong>0.12 of drug loading, releasing about 57.72%<strong>±</strong>1.17 at pH 5.2 and only 14.14%<strong>±</strong>1.32 at pH 7.4 after 48 h. No significant change in stability was observed after storage at 5 °C±3 °C as well as at 25 °C±2 °C/60% RH±5% RH for 6 mo. The cytotoxicity effect of L-NLs was higher by 10% then that of marketed formulation at 10 µg/ml docetaxel concentration. Fluorescence microscopic investigation showed that more cellular uptake and apoptotic effect were observed in L-NLs treated C6 glial cells than in those treated with the marketed formulation.</p> <p><strong>Conclusion: </strong>HA-DTX loaded nanoliposomes enabled docetaxel to target C6 glial cells with better efficacy and might be effective to treat glioma.</p>}, number={6}, journal={International Journal of Applied Pharmaceutics}, author={SEIFU, MULUNEH FROMSA and NATH, LILA KANTA and DUTTA, DEBASHIS}, year={2020}, month={Nov.}, pages={88–99} }