TY - JOUR AU - Shiyan, Shaum AU - Hertiani, Triana AU - Martien, Ronny AU - Nugroho, Akhmad Kharis PY - 2018/11/07 Y2 - 2024/03/28 TI - OPTIMIZATION OF A NOVEL KINETIC-ASSISTED INFUNDATION FOR RICH-EGCG AND POLYPHENOLS OF WHITE TEA (CAMELLIA SINENSIS) USING CENTRAL COMPOSITE DESIGN JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 10 IS - 6 SE - Original Article(s) DO - 10.22159/ijap.2018v10i6.29654 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/29654 SP - 259-267 AB - <p class="Default"><strong>Objective: </strong>This research aimed to find modeling and optimization of a novel kinetic-assisted infundation for rich-epigallocatechin gallate (EGCG) and polyphenols extraction from white tea leaf (<em>Camellia sinensis</em><em> </em>L.).</p><p class="Default"><strong>Methods: </strong>The optimal conditions for the best extraction of kinetic-assisted infundation were determined using central composite design. The content of EGCG, catechin, gallic acid, caffeine, theobromine, and theophylline was quantified using high-performance liquid chromatography with ultraviolet detection (HPLC/UV-detection). The total polyphenolic content (TPC) and total flavonoid content (TFC) was measured using the spectrophotometric method. The semi-purified extract was characterized by HPLC, fourier transform infrared spectrophotometry-universal attenuated total reflectance (FTIR-UATR), and powder-X ray diffraction (P-XRD). The extraction mechanism of target compounds was analyzed using scanning electron microscopy (SEM) qualitatively.</p><p class="Default"><strong>Results: </strong>The optimum process for the rich-EGCG and polyphenolic extraction using kinetic-assisted infundation was the concentration of simplicia 14.75 %, the stir rate 440 rpm, and extraction time 12.31 min. The yield of extracts, TPC, TFC, EGCG, caffeine, gallic acid, and theobromine contents in the optimal extraction process were 9.34 %, 70.97 %, 13.95 %, 29.02 %, 11.95 %, 1.33 %, and 0.33 %, respectively.</p><p class="Default"><strong>Conclusion: </strong>The kinetic-assisted infundation method proved to be easy to apply with good results as an alternative technique for extracting polyphenolic compounds and rich-EGCG from white tea leaves.</p> ER -