TY - JOUR AU - DJAIS, ARIADNA ADISATTYA AU - JEMMY, AU - PUTRI, NADHIFA AU - PUTRI, ANDIN RAHMANIA AU - DARWITA, RISQA RINA AU - BACHTIAR, BOY MUCHLIS PY - 2019/04/05 Y2 - 2024/03/29 TI - BIOFILM FORMATION OF CANDIDA ALBICANS EXPOSED TO ETHANOL EXTRACT OF PROPOLIS JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 11 IS - 1 SE - Original Article(s) DO - 10.22159/ijap.2019.v11s1.18344 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/33435 SP - 210-213 AB - <p><strong>Objective</strong>: Propolis extract showed an excellent in vitro performance against yeast and was additionally found to be fungistatic and fungicidal. Propolis<br>extract is also used for treatment and prevention of fungal infections. However, its effectiveness against Candida albicans biofilm formation requires<br>investigation. The study evaluated the ability of propolis to inhibit C. albicans while the fungus is growing as a biofilm in vitro.<br><strong>Methods</strong>: Two reference strains, C. albicans ATCC 25923 and a clinical strain (laboratory stock), were used in this study. For the biofilm experiment,<br>the fungi were cultured in Tryptic Soy Broth medium with 1% sucrose and incubated at 37°C for 24 h, and different concentrations of ethanol extract<br>of propolis were used as the inhibitor agents. Biofilm assays were performed in 96-well microtiter plates, quantification of the total biofilm biomass<br>was performed using a crystal violet staining method, and the Student’s t-test was chosen for statistical analyses.<br><strong>Results</strong>: Our data showed that 3 h incubation with propolis did not affect the biomass in the experimental group compared to the control. When the<br>incubation time was extended to 18 h, the biomass increased significantly compared to the control.<br><strong>Conclusion</strong>: This study showed that several concentrations of propolis did not inhibit biofilm. However, in each incubation time, we observed no<br>hyphal morphology in the biofilm mass. Propolis might attenuate the opportunistic virulence of fungus growing as a biofilm in vitro. Further studies<br>are necessary to confirm this phenomenon.</p> ER -