TY - JOUR AU - BAO, JIBO AU - H. E., JUN AU - L. I., ZILIANG AU - YANG, RONGQIANG AU - ZOU, JINYANG AU - DING, YIXIN AU - NIU, TAO AU - XIE, ZHIGANG AU - SERIWATANACHAI, DUTMANEE PY - 2019/04/05 Y2 - 2024/03/29 TI - BONE MORPHOGENETIC PROTEIN-2 AND COLLAGEN TYPE 1 FROM DIFFERENT SOURCES OF DEMINERALIZED DENTINE MATRIX: RELEASE KINETIC AND CHEMOTAXIS POTENTIAL FOR OSTEOPROGENITOR CELLS JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 11 IS - 1 SE - Original Article(s) DO - 10.22159/ijap.2019.v11s1.18475 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/33439 SP - 223-229 AB - <p><strong>Objective</strong>: To investigate the release of bone morphogenetic protein-2 (BMP-2) and collagen type I proteins (COL1) from different sources of<br>demineralized dentine matrix (DDM) and their chemotaxis to mouse osteoprogenitor cells.<br><strong>Methods</strong>: The release kinetic of BMP-2 and COL1 was measured from custom-made DDM (CMDDM) and commercially available DDM (CADDM).<br>Using Urist physicochemical method, CMDDM was collected from the extracted teeth in a certified dental clinic. Levels of BMP-2 and COL1 released<br>were measured at days 1, 2, 3, 5, 7, 9, 11, and 13. Next, mouse osteoprogenitor cells, MC3T3-E1, were cultured with a variety of materials as follows:<br>CMDDM, CADDM, Bio-Oss®, and blank control in transwell system. The number of cell migration was determined by crystal violet staining to explore<br>chemotaxis of different DDMs to mouse osteoprogenitor cells.<br><strong>Results</strong>: BMP-2 was detected at 588.32 ± 14.53 pg/ml from 5 g of CMDDM. In the release kinetic assay, the concentration of BMP-2 in the CMDDM<br>group increased rapidly and peaked at 113.9 pg/ml on day 5, almost four times higher than that of CADDM. The release of COL1 showed similar<br>pattern in both CMDDM and CADDM; however, the amount was significantly higher in the CMDDM group. In cell culture experiment, the number of<br>migrated MC3T3-E1 was ranked as the highest in CMDDM, followed by CADDM and Bio-Oss® (p&lt;0.05).<br><strong>Conclusion</strong>: CMDDM released BMP-2 and COL1 greater than CADDM, which can induce more osteoblast-like cell migration. These results demonstrated<br>a release kinetic of proteins and osteoinductivity of CMDDM, which supports a benefit of using autogenous bone graft.</p> ER -