TY - JOUR AU - V. S., LAKSHMI AU - MENON, REVATHY B. AU - RAJU, KEERTHANA AU - M. U., AISWARYA AU - C. NAIR, SREEJA PY - 2019/09/07 Y2 - 2024/03/29 TI - FORMULATION AND EVALUATION OF LORAZEPAM ENCAPSULATED COLLAGEN/PECTIN BUCCAL PATCH JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 11 IS - 5 SE - Original Article(s) DO - 10.22159/ijap.2019v11i5.34366 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/34366 SP - 200-209 AB - <p><strong>Objective: </strong>To formulate and characterize Lorazepam loaded buccal patches using mucoadhesive, biodegradable, natural polymers-pectin (hydrophilic) and collagen (lipophilic) for treating epileptic seizures.</p><p><strong>Methods: </strong>Lorazepam loaded buccal patches were prepared by solvent casting method and were subjected to various Physico-chemical evaluation parameters to find the optimized buccal patch. The <em>in vitro</em> drug release study and <em>ex vivo</em> permeation study was carried out. The stability study and histopathological study of optimized Lorazepam loaded buccal patch was also carried out.</p><p><strong>Results: </strong>From <em>in vitro </em>drug release study, it was found that Lorazepam loaded buccal patch (B4) exhibited maximum drug release of 96.16 %±0.07 than other formulations at the end of 4 h, indicating an initial burst release followed by sustained release with release kinetics as Higuchi diffusion model. Based on the <em>in vitro</em> drug release, % drug content, % swelling index, folding endurance, B4 formulation was considered as optimised formulation and was further characterized. <em>Ex vivo</em> permeation study revealed that the cumulative amount of drug permeated from optimised Lorazepam loaded buccal patch (B4) was higher (3831.4±0.21µg/cm<sup>2</sup>) than marketed Midazolam buccal solution (1724±0.12 µg/cm<sup>2</sup>) and control drug solution (895.42±0.07 µg/cm<sup>2</sup>) with an enhancement ratio of 4.8. B4 formulation also showed a higher flux value (12.52±0.02µg/cm<sup>2</sup>/hr) compared to marketed formulation (5.732±0.01 µg/cm<sup>2</sup>) and control drug solution (2.563±0.03 µg/cm<sup>2</sup>) of P&lt;0.05. The histopathological study using bovine buccal mucosa revealed that the B4 formulation is safe for buccal application. The stability study confirmed that B4 formulation is stable in both room and refrigeration conditions. Hence the formulated Lorazepam loaded buccal patch seems to be a promising carrier for the enhanced buccal delivery of Lorazepam in treating epileptic seizures.</p><p><strong>Conclusion: </strong>The formulated Lorazepam loaded collagen/pectin buccal patch was found to be an efficient and stable route for the buccal delivery of Lorazepam in treating acute epileptic seizures which could be further explored scientifically.</p> ER -