TY - JOUR AU - JASIKOVA, DARINA AU - RYSOVA, MIROSLAVA AU - KOTEK, MICHAL PY - 2019/09/15 Y2 - 2024/03/28 TI - APPLICATION OF LASER-INDUCED BREAKDOWN CAVITATION BUBBLES FOR CELL LYSIS IN VITRO JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 11 IS - 5 SE - Original Article(s) DO - 10.22159/ijap.2019.v11s5.T3059 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/35860 SP - 186-190 AB - <p><strong>Objective</strong>: Understanding the basic mechanism of the cavitation bubble action on living cells as a crucial step of development and application of<br>sophisticated methods based on controlled cavitation in cell behaviour manipulation. Optimisation of parameters in order to expand cell lysis region<br>created by a single bubble.<br><strong>Methods</strong>: The cavitation bubbles are generated by the laser-induced breakdown method. The impact of controlled cavitation bubble on the<br>biological system is synchronously monitored under a microscope and recorded. Visualization of the cavitation bubble course is monitored by a highspeed<br>camera. The impact of technology on the healthy confluent cell layer is verified. Evaluation of the cavitation bubbles´ effect on cells in real time<br>and by subsequent analysis of the cell lysis region and impact of the cavitation bubble on cell viability is carried out by optical visualization and life/<br>dead fluorescence staining.<br><strong>Results</strong>: Cavitation bubble induced in distance of 1.5 mm from the cell surface overcomes properties of sessile bubble and enables to create cell lysis<br>region over 1000 μm in diameter due to transient shear stress produced by liquid displaced by the bubble expansion.<br><strong>Conclusion</strong>: Cell lysis region is strongly dependent on the spot laser energy (SLE) and the bubble induction distance from cells. This knowledge is<br>crucial for application in chemical free cell lysis in vitro, wound induction for experimental purposes and cell layers patterning in desired scale.</p> ER -