TY - JOUR AU - SAKTI, ADITYA SINDU AU - WIDYASTANTO, HARNANDA AU - MAULIDINA, ASTRI AU - MITASARI, DIAN AU - ERIYANI, DIYAH SANTI AU - MUN’IM, ABDUL PY - 2020/03/23 Y2 - 2024/03/29 TI - XANTHINE OXIDASE INHIBITORY ACTIVITY OF METHANOL EXTRACT FRACTIONS OF VARIOUS INDONESIAN ETHNOPHARMACOLOGICAL PLANTS JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 12 IS - 1 SE - Original Article(s) DO - 10.22159/ijap.2020.v12s1.FF004 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/37447 SP - 43-46 AB - <p><strong>Objective</strong>: Hyperuricemia involves an increase in serum uric acid levels, resulting in kidney damage, increased mortality, and reduced quality of life.<br>Inhibitors of xanthine oxidase, which catalyzes the last step in uric acid synthesis, are targets for therapeutic intervention.<br><strong>Methods</strong>: An ethnopharmacological approach, screening four native Indonesian herbal medicinal plants with reported activity against hyperuricemia,<br>was used for preliminary studies, fractionating methanolic extracts by solvent partitioning. Fractions were then tested in vitro for xanthine oxidase<br>inhibitory activity, and the most active fraction was then subjected to preliminary phytochemical screening.<br><strong>Results</strong>: The target tissue of the four herbal medicinal plants investigated was Indian bay leaf (Syzygium polyanthum Wight.), God’s crown fruit<br>(Phaleria macrocarpa Boerl.), snake fruit peel (Salacca edulis Reinw.), and Job’s tears tuber (Cyperus rotundus Linn.). Each sample was extracted by<br>maceration with 80% methanol. The concentrated extract was then fractionated by the liquid-liquid partition method (1:1 v/v) using n-hexane, ethyl<br>acetate, butanol, and methanol sequentially as solvents. The results revealed that the ethyl acetate fraction was the most active fraction. S. polyanthum<br>leaf and C. rotundus tuber showed the greatest potential in inhibiting xanthine oxidase, with half-maximal inhibitory concentrations of 18.43 and<br>10.50 μg/ml, respectively. Enzyme kinetics analysis shows that each plant fraction works as a competitive inhibitor of xanthine oxidase.<br><strong>Conclusion</strong>: Preliminary screening identified the ethyl acetate fractions of two native Indonesian herbal medicinal plants as showing potential for<br>anti-hyperuricemia activity.</p> ER -