TY - JOUR AU - MARDHIYAH, SITI AU - ELYA, BERNA AU - NOVIANI, ARIKADIA PY - 2020/03/23 Y2 - 2024/03/29 TI - ELASTASE ACTIVITY INHIBITION BY THE MOST ACTIVE FRACTION OF STAR FRUIT (AVERRHOA CARAMBOLA L.) LEAVES FROM THREE WEST JAVA REGIONS JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 12 IS - 1 SE - Original Article(s) DO - 10.22159/ijap.2020.v12s1.FF020 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/37545 SP - 101-106 AB - <p><strong>Objective</strong>: Premature skin aging is caused by increased elastase proteolytic activity, which causes elastin breakdown and disorganization in connective<br>tissue, reducing elasticity and flexibility, and wrinkling skin. Natural compounds in plants, especially polyphenols, inhibit elastase proteolytic<br>activity and prevent premature skin aging. Star fruit (Averrhoa carambola L.) leaves contain many polyphenols with antioxidant, anti-inflammatory,<br>hypoglycemic, and antimicrobial activities. However, no studies have shown that A. carambola leaves inhibit elastase proteolytic activity.<br><strong>Methods</strong>: This study tested the inhibition of elastase proteolytic activity by the water fractions (WF), ethyl acetate fractions, and n-hexane fractions<br>of A. carambola leaves from the Depok, Sukabumi, and Subang regions of West Java. Each fraction was tested using a microplate reader, and the total<br>phenolic and flavonoid content was determined for the most active fraction.<br><strong>Results</strong>: The WF of the A. carambola leaves from Depok was the most active fraction, with a half-maximal inhibitory concentration of 160.36 μg/mL.<br>The total phenolic and flavonoid content in the WF was 115.68 mg gallic acid equivalent/g extract and 9.15 mg quercetin equivalent/g extract,<br>respectively.<br><strong>Conclusion</strong>: The WF of A. carambola leaves is a natural material that may inhibit elastase proteolytic activity and prevents premature skin aging.</p> ER -