TY - JOUR AU - SUBARDINI, DEFI SUCIANA AU - ELYA, BERNA AU - NOVIANI, ARIKADIA PY - 2020/03/23 Y2 - 2024/03/29 TI - LIPOXYGENASE INHIBITORY ASSAY OF ETHYL ACETATE FRACTION FROM STAR FRUIT LEAVES (AVERRHOA CARAMBOLA L.) FROM THREE REGIONS IN WEST JAVA JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 12 IS - 1 SE - Original Article(s) DO - 10.22159/ijap.2020.v12s1.FF025 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/37550 SP - 119-121 AB - <p><strong>Objective</strong>: Averrhoa carambola L. leaves have been used as a traditional medicine. The study aimed to examine the anti-inflammatory activity of<br>A. carambola L. plant extracts using a lipoxygenase inhibition assay based on ethyl acetate fractions of A. carambola L. leaves obtained from three<br>regions in West Java.<br><strong>Methods</strong>: Extracts from A. carambola L. leaves were obtained by maceration method using 70% ethanol followed by fractionation using liquid-liquid<br>partitioning until the ethyl acetate fraction was obtained. Subsequently, lipoxygenase inhibition activity of the ethyl acetate fraction was tested using<br>linoleate acid as the substrate, and the total flavonoid and total phenol contents were determined. The correlation between the IC50 values and the<br>total flavonoid and total phenol contents obtained from the ethyl acetate fraction were analyzed.<br><strong>Results</strong>: The ethyl acetate fraction from the sample inhibited lipoxygenase enzyme activity with IC50 values of 19.38, 16.65, and 15.07 μg/mL, respectively.<br>The total flavonoid contents in the ethyl acetate fractions obtained from Depok, Subang, and Sukabumi regions were 14.88, 16.88, and 22.27 mg QE/g<br>of sample, respectively, whereas the total phenol contents were 54.10, 61.06, and 72.18 mg gallic acid equivalents/g of sample, respectively.<br><strong>Conclusion</strong>: There was a high correlation between the IC50 values and the total flavonoid and total phenol contents, with correlations coefficients<br>of −0.917 and −0.960, respectively, which indicated that the higher the total flavonoid and total phenol contents, the lower the IC50 values.</p> ER -