TY - JOUR AU - HARMITA, HARMITA AU - HAYUN, HAYUN AU - GEOFANI, M. HERU PY - 2020/03/23 Y2 - 2024/03/29 TI - QUANTIFICATION OF HYALURONIC ACID AND METHYLSULFONYLMETHANE IN DIETARY SUPPLEMENTS JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 12 IS - 1 SE - Original Article(s) DO - 10.22159/ijap.2020.v12s1.FF032 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/37557 SP - 143-148 AB - <p><strong>Objective</strong>: Osteoarthritis can be treated by taking oral supplements containing compounds that can nourish bones and joints such as hyaluronic acid,<br>methylsulfonylmethane (MSM), chondroitin, glucosamine, and collagen. This study aimed to develop and validate tests for analyzing two compounds,<br>namely, hyaluronic acid and MSM, simultaneously and to determine both their levels in a mixed sample.<br><strong>Methods</strong>: Hyaluronic acid derivatization was carried out using fluorenylmethyloxycarbonyl chloride and then analyzed by liquid chromatography<br>with fluorescence detection, while MSM was analyzed using gas chromatography. After the development of optimal conditions for each separation,<br>system suitability tests were developed and calibration curves used for tests of accuracy and precision as well as for level determination. Hyaluronic<br>was detected at an excitation wavelength of 255 nm and emission wavelength of 330 nm. The mobile phase used was acetonitrile-acetate pH 4.2 (1: 4)<br>with a flow rate of 1.0 mL/min.<br><strong>Results</strong>: The developed method was linear (r=0.9983) in the range of 5–50 ppm and the limits of detection (LOD) and quantitation (LOQ) were 3.55<br>and 11.84 ppm, respectively. The initial column temperature for MSM analysis was 110°C and the mobile phase used was nitrogen gas at a flow rate of<br>0.8 mL/min. The method was linear (r=0.9998) in the range of 4000–15,000 ppm and the LOD and LOQ were 332.90 and 1109.67 ppm, respectively.<br><strong>Conclusion</strong>: A simulated sample containing both compounds was assessed to contained 98.63% hyaluronic acid and 99.35% MSM.</p> ER -