TY - JOUR AU - HARMITA, HARMITA AU - ISKANDARSYAH, ISKANDARSYAH AU - AFIFAH, SHOFIYAH FATIN PY - 2020/03/23 Y2 - 2024/03/29 TI - EFFECT OF TRANSFERSOME FORMULATION ON THE STABILITY AND ANTIOXIDANT ACTIVITY OF N-ACETYLCYSTEINE IN ANTI-AGING CREAM JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 12 IS - 1 SE - Original Article(s) DO - 10.22159/ijap.2020.v12s1.FF034 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/37559 SP - 156-162 AB - <p><strong>Objective</strong>: N-acetylcysteine is an antioxidant with thiol/sulfhydryl groups and is currently being developed as an active ingredient in anti-aging<br>creams. The study’s aim was to compare the stability and antioxidant activity of N-acetylcysteine in anti-aging creams formulated with and without<br>a transfersome carrier system.<br><strong>Methods</strong>: Stability was assessed by performing cycling, centrifugal, and accelerated stability tests. In addition, antioxidant activity was measured<br>by the DPPH method, and in vitro penetration was measured using Franz diffusion cells. The analysis of N-acetylcysteine was performed using highperformance<br>liquid chromatography with ultraviolet–visible detection at a wavelength of 214 nm and a flow rate of 1.0 mL/min, injection volume of<br>5 μL, and a mobile phase of phosphate buffer pH 3.0.<br><strong>Results</strong>: The N-acetylcysteine transfersome and non-transfersome cream preparations did not change color or show phase separation during the<br>cycling and centrifugal tests. The N-acetylcysteine in the transfersome and non-transfersome cream preparations had strong antioxidant activity,<br>with half-maximal inhibitory concentrations of 26.90 μg/mL and 38.63 μg/mL, respectively. The in vitro penetration test using Franz diffusion cells<br>showed that the cumulative amount of penetrated N-acetylcysteine was 7355.13 μg/cm2 (flux of 845.67 μg/cm2∙h) in the transfersome cream and<br>4677.61 μg/cm2 (flux of 533.33 μg/cm2∙h) in the non-transfersome cream.<br><strong>Conclusion</strong>: The in vitro penetration test results showed that the transfersome formulations in creams were able to increase the cumulative amount<br>and flux of penetrated N-acetylcysteine in anti-aging cream preparations relative to those not formulated with transfersome.</p> ER -