TY - JOUR AU - HIDAYATI, ARI ARIEFAH AU - ELYA, BERNA AU - SAURIASARI, RANI PY - 2020/03/23 Y2 - 2024/03/29 TI - SYZYGIUM CUMINI (L.) SKEELS LEAF EXTRACT FRACTIONS AS ARGINASE INHIBITORS AND THE EFFECTS OF TANNINS ON THEIR ACTIVITY JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 12 IS - 1 SE - Original Article(s) DO - 10.22159/ijap.2020.v12s1.FF059 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/37605 SP - 268-270 AB - <p><strong>Objective</strong>: Arginase inhibition could be a potential therapeutic approach for endothelial dysfunction. Syzygium cumini (L.) Skeels leaves contain<br>phenolic acids and flavonoids, which have been predicted to exhibit arginase inhibitory activity. Moreover, these leaves contain tannins, which can<br>form complexes with enzymes and lead to false-positive results during biological testing. Therefore, this study was conducted to evaluate the arginase<br>inhibitory activity of S. cumini leaf extract and fractions as well as to elucidate the effects of tannins on this activity.<br><strong>Methods</strong>: S. cumini leaves were fractionated using n-hexane, ethyl acetate, and methanol. A colorimetric method was employed to evaluate arginase<br>inhibitory activity. Tannin elimination was performed through the gelatin precipitation method. Total phenolic and flavonoid contents of the fractions<br>were calculated using the Folin–Ciocalteu and aluminum chloride methods, respectively.<br><strong>Results</strong>: Ethyl acetate and methanol fractions showed arginase inhibitory activity with half-maximal inhibitory concentrations (IC50) of 46.96 and<br>15.35 μg/mL, respectively. The methanol fraction was positive for tannins. After tannin elimination, this fraction exhibited less potent arginase<br>inhibitory activity, with an IC50 value of 53.03 μg/mL. The ethyl acetate fraction showed higher total phenolic and flavonoid contents than the methanol<br>fraction.<br><strong>Conclusion</strong>: Tannins affected the arginase inhibitory activity of the methanol fraction of S. cumini leaves; however, the ethyl acetate fraction did not<br>contain tannins and could inhibit arginase activity.</p> ER -