TY - JOUR AU - BINARTHA, CIPTADHI TRI OKA AU - SUPRASTIWI, ENDANG AU - KURNIA, DIDIK AU - MARGONO, ANGGRAINI AU - ARTININGSIH, DEWA AYU NYOMAN PUTRI PY - 2020/03/23 Y2 - 2024/03/29 TI - ANTIBACTERIAL EFFECTS OF SARANG SEMUT (MYRMECODIA PENDANS) FRACTIONS USING THREE DIFFERENT SOLVENTS TOWARD ENTEROCOCCUS FAECALIS CPS2 JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 12 IS - 1 SE - Original Article(s) DO - 10.22159/ijap.2020.v12s1.iwdr01 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/37606 SP - 271-275 AB - <p><strong>Objective</strong>: This study investigated the effect of antibacterial activity from sarang semut fractions with three different solvents, i.e. nonpolar (n-hexane),<br>semipolar (ethyl acetate), and polar (water), to determine the minimum inhibitory concentration (MIC) on Enterococcus faecalis cps2.<br><strong>Methods</strong>: The fractions were extracted with a maceration method and a methanol solvent. The fractionation was performed with three groups<br>of solvent to obtain the n-hexane, ethyl acetate, and water fractions. The active compound from the best fraction group was identified using a<br>phytochemical test, gas chromatography-mass spectrophotometry, and thin-layer chromatography. Each fraction group was divided into five different<br>concentrations, i.e. 20%, 40%, 60%, 80%, and 100% and was assessed against E. faecalis cps2 with an agar diffusion method. Chlorhexidine 2% was<br>used as a positive control. The width of the inhibition zone was calculated.<br><strong>Results</strong>: The ethyl acetate group had the biggest inhibition zone of 21 mm in diameter compared to n-hexane and water, which was 15 mm and<br>19 mm in diameter, respectively. The MIC value of the fraction with a 20% concentration of ethyl acetate was significantly different (P &lt; 0.05) from the<br>n-hexane and water solvents in inhibiting the growth of E. faecalis cps2.<br><strong>Conclusion</strong>: The ethyl acetate fraction of sarang semut had a greater inhibitory effect on E. faecalis cps2. In addition, the antibacterial activity of the<br>fraction increased with an increase in concentration.</p> ER -