TY - JOUR AU - FITRI, MAIDA AU - NAZAR, KAMIZAR AU - MEIDYAWATI, RATNA AU - AZMI, REZA PY - 2020/07/24 Y2 - 2024/03/28 TI - ANTIBACTERIAL EFFECT OF XANTHORRHIZOL (CURCUMA XANTHORRHIZA ROXB.) AGAINST THE BIOFILM OF FUSOBACTERIUM NUCLEATUM JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 12 IS - 2 SE - Original Article(s) DO - 10.22159/ijap.2020.v12s2.PP-06 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/39176 SP - 57-61 AB - <p><strong>Objective</strong>: Endodontic infections are treated with a root canal; one of the stages involves using an irrigation solution to eliminate microorganisms.<br>Sodium hypochlorite (NaOCl) is an irrigation solution used to eliminate Fusobacterium nucleatum, the common cause of primary endodontic<br>infections. However, this material is synthetic and can cause side effects and inflammation of the periapical tissues. Thus, an irrigation solution, such<br>as xanthorrhizol, which is made of natural ingredients, is required. However, xanthorrhizol’s antibacterial effect against F. nucleatum has never been<br>assessed. Thus, this study aimed to analyze the effect of xanthorrhizol, derived from Curcuma xanthorrhiza Roxb., against F. nucleatum ATCC 25586<br>biofilm.<br><strong>Methods</strong>: The methylthiazol tetrazolium (MTT) assay test and colony count test were performed to assess F. nucleatum eradication after exposure to<br>xanthorrhizol at various concentrations (0.5%, 0.75%, 1%, 1.25%, and 1.5%) and 2.5% NaOCl, which was used as positive control.<br><strong>Results</strong>: Significant differences were observed in terms of cell viability after treatment with xanthorrhizol at concentrations of 1.25% versus 0.5%,<br>0.75%, and 1%. The MTT assay test was used to evaluate cell viability in the biofilm and cell metabolism activities. Results showed no significant<br>differences in terms of efficacy between xanthorrhizol at concentrations of 1.25% and 1.5%, which are considered effective against F. nucleatum, and<br>2.5% NaOCl (p&gt;0.05).<br><strong>Conclusion</strong>: The effect of xanthorrhizol at concentrations of 1.25% and 1.5% against F. nucleatum is similar to that of 2.5% NaOCl.</p> ER -