TY - JOUR AU - POUYFUNG, PHISIT AU - SUKATI, SURIYAN PY - 2021/01/31 Y2 - 2024/03/29 TI - ANTI-COAGULANT PROPERTIES OF FLAVONOID COMPOUNDS: POTENTIAL STRUCTURE-FUNCTIONAL RELATIONSHIP JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 13 IS - 1 SE - Full Proceeding Paper DO - 10.22159/ijap.2021.v13s1.Y0050 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/40929 SP - 9-12 AB - <p><strong>Objective</strong>: Flavonoids, naturally-occurring compounds in fruits and vegetables, possess anti-coagulant property. However, a very few studies were<br>attempted to understand how flavonoid structure influences its anti-coagulation property, such as clotting time. In this study, we investigated<br>structurally similar flavonoid compounds which differ in the number of hydroxyl groups and compared their anti-coagulation properties.<br><strong>Methods</strong>: We selected and evaluated five flavonoid compounds, that is, chrysin, apigenin, luteolin, kaempferol, and quercetin, for their anti-coagulant<br>properties using in vitro prothrombin time (PT) assays and activated partial thromboplastin time (APTT) assay.<br><strong>Results</strong>: Our findings suggested that quercetin, kaempferol, and luteolin showed a significant anti-coagulant effect on APTT (p&lt;0.05) in a dosedependent<br>manner. The dose of 500 μM quercetin showed potent prolong APTT with 37.43±1.60 s, followed by 500 μM of kaempferol and luteolin<br>(34.63±1.29 s and 4.83±1.56 s, respectively). Furthermore, a combination of 500 μM of quercetin with 0.25 U/ml of heparin demonstrated prolong<br>APTT (52.16±5.18 s) when compared with individual effects of either 0.25 U/ml heparin (33.4±0.50 s) or 500 μM quercetin (37.43±1.62 s) alone.<br><strong>Conclusion</strong>: Our results demonstrated that numbers of the hydroxyl group on flavonoid compounds influence anti-coagulation properties. In<br>addition, the prolonged APTT assay results suggested that quercetin, kaempferol, and luteolin could affect factors VIII, IX, XI, and XII of intrinsic<br>pathway. Moreover, the synergistic effect of quercetin further enhances the heparin anti-coagulation effect. Based on our findings, we recommend<br>that the consumption of vegetables and fruits rich in quercetin, luteolin, and kaempferol could help prevent thrombotic stroke in high-risk patients.</p> ER -