TY - JOUR AU - SINGH, RAHUL KUMAR AU - DEORA, ANIRUDH SINGH PY - 2022/07/07 Y2 - 2024/03/29 TI - 5-FLUOROURACIL IMPREGNATED NIOSOMAL-IN SITU GEL (THERMO-SENSITIVE) FOR ORAL CANCER: DESIGN, CHARACTERIZATION, IN VITRO/EX VIVO EVALUATION JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 14 IS - 4 SE - Original Article(s) DO - 10.22159/ijap.2022v14i4.44195 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/44195 SP - 126-137 AB - <p><strong>Objective</strong>: To formulate and characterize 5-fluorouracil loaded niosomal-<em>in-situ</em> gel for sustained drug delivery to reduce dosing frequency at the same time, follow a local drug delivery for cancer targeting.</p><p><strong>Methods</strong>: Cholesterol and span-60-based niosomes were prepared after following the modified ether injection method. Best formulation selected after characterization through FTIR, SEM, % Entrapment efficiency, zeta potential, polydispersity index, <em>in vitro</em> release, and vesicle size whereas, based on cold method niosomes encapsulated in-situ gel was formulated and characterized through gelling temperature and time, spreadability and syringe ability, gel strength, adhesive force, and drug release.</p><p><strong>Results</strong>: Based on various studies, included particle size, PDI, zeta potential value, % Entrapment efficiency and % drug release, F1 formulation was selected as a best formulation, as niosomal particle size of 388.3 nm proved a higher drug permeation through the buccal area, whereas PDI and zeta potential value of 0.304 and+50.5 are proved a uniform niosomal size with optimum charge distribution which helps to attain higher stability of the formulation, on the other hand % Entrapment efficiency of 87.825% proved that niosomes are capable to hold higher drug concentration; lastly 84.567% of drug release within 12 h of time period prove that higher amount of drug release occur by following sustained release pattern. On the other hand Mucoadhesion, gelling strength and <em>in vitro</em> permeation studies prove that niosomes containing in-situ gel has a capacity to adhere over the mucosa with minimum dissolution with saliva up to 12 h and is capable of 95% of drug permeation capacity. Lastly FTIR and SEM images confirmed about niosomal formation with optimum stability.</p><p><strong>Conclusion</strong>: 5-Fluorouracil encapsulated niosomal in-situ gel will be superior and effective alternative to parenteral dosage forms available in the market for mouth cancer treatment.</p> ER -