TY - JOUR AU - ZUHROTUN, ADE AU - HADISAPUTRI, YUNI ELSA AU - ALEXANDER, FREDERICK AU - SUSENO, RUDY AU - WICAKSONO, IMAM ADI PY - 2022/11/26 Y2 - 2024/03/28 TI - TOPOISOMERASE INHIBITORS ACTIVITY OF YELLOW CHAMPAKA (MICHELIA CHAMPACA L.) BARK EXTRACTS AND FRACTIONS AND ITS LIRIODENINE CONTENTS JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 14 IS - 4 SE - Original Article(s) DO - 10.22159/ijap.2022.v14s4.PP37 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/46941 SP - 151-154 AB - <p><strong>Objective: </strong>This research is aimed to investigate the activity of various extracts and fractions of yellow champaka (<em>Michelia champaca</em> L.) by mechanism-based yeast bioassay (MBYB) against the mutant yeasts. Liriodenin content, an active compound in <em>M. champaca</em>, will analyze to know the correlation with this assay.</p><p><strong>Methods: </strong>The bark of <em>M. champaca</em> was extracted by maceration and graded maceration using n-hexane, ethyl acetate, and methanol. The methanol extract was fractionated using water, n-hexane and ethyl acetate in the liquid-liquid extraction process. Each extract and fractions were tested <em>in vitro</em> by mechanism-based yeast bioassay for their topoisomerase inhibitor activity. Liriodenine content of all samples was analyzed by thin layer chromatography (TLC) densitometry method.</p><p><strong>Results: </strong>The yeast bioassay results showed that all extracts were active as topoisomerase inhibitors (IC<sub>12</sub> values under 8000 µg/ml) except MGM. Liriodenine can be used as a marker of active compound in ethyl acetate samples that were EAM, EAGM and EAF with Pearson analysis value-0.887 (P=0.153) and these samples were relatively more active than others.</p><p><strong>Conclusion: </strong>This research showed that various extracts and fractions of yellow champaka bark have topoisomerase inhibitor's activity. Liriodenine can be used as a marker of the active compound so yellow champak bark is a potent natural agents for breast cancer.</p> ER -