ISOLATION, IDENTIFICATION AND ANTIMICROBIAL ACTIVITY OF BIOACTIVE COMPOUND FROM SARACA ASOCA
Objective: Saraca asoca which is also called Saraca indica is the ancient tree of the family Caesalpinaceae. This tree is considered as the first in medicinal values since the Buddist period. Ashoka is traditionally used in the treatment of excessive uterine bleeding, dysmenorrhoea and depression in women. It is also used to treat inflammation, indigestion and also abnormal vaginal discharge. It is found to have antimicrobial, anticancer, antimenorrhagic, anti oxytocic activities. The study focuses on to isolate and characterizes the bioactive compounds from the leaves of Saraca asoca by cold extraction method.
Methods: The crude extracts of ethanol, petroleum ether, and aqueous extracts were spotted in TLC plate to fig. out the number of probable compounds in each extract. Column chromatography was performed to collect purified fractions. Phytochemical screening of crude samples were tested, the presence of flavonoids, steroids, tannins, alkaloids, glycosides, phenols, reducing sugars were identified. Antimicrobial activity of ethanol, petroleum ether fractions were tested using agar-well diffusion method, maximum activity was recorded in a concentration-dependent manner against Escherichia coli, Staphylococcus sp, Streptococcus sp, Pseudomonas sp, Proteus sp, Bacillus sp, Aspergillus sp and Candida sp.
Results: Among the total fractions obtained, few active fractions inhibited growth of organisms which would significantly serve as a potential source for new antimicrobial compounds. GCMS was performed to identify the potential compound to be Phytol exhibiting antimicrobial activity.
Conclusion: The antimicrobial activity of the bioactive compounds was tested against various microorganisms and the diameter of the zone obtained was calculated where the petroleum ether fractions exhibited good inhibition and ethanolic fractions does not have inhibitory activity.Keywords: Saraca asoca, Ethanol, Chloroform, Petroleum ether, Ethyl acetate, Antimicrobial activity.
2. Anjum Gahlaut, Rajesh Dabur, Anil K Chhillar. Anti-aspergillus activity of selected medicinal plants. J Pharm Res 2013;6:1-4.
3. Hawas U, Eltomy S, Nassif R, El-Hossary G, Abou Zid S. Retraction. A new antifungal labdane diterpene from the leaves of Saracaindica. Nat Prod Res 2015;29:i-vi.
4. Thongtan J, Kittakoop P, Ruangrungsi N, Saenboonrueng J, Thebtaranonth Y. New antimycobacterial and antimalarial 8,9-secokaurane diterpenes from Crotonkongensis. J Nat Prod 2003;66:868-70.
5. Jayita Saha, Taniya Mitra, Kamala Gupt, Sumona Mukherjee. Phytoconstituents and HPTLC analysis in Saraca asoca(Roxb.) wilde. Int J Pharm Pharm Sci 2012:4;96-9.
6. P Pradhan, Joseph L, Gupta V, Chulet R, Arya H, Verma R, et al. Saraca asoca (Ashoka): a review. J Chem Pharm Res 2009;1:62-71.
7. Singh S, Sarma B, Singh U, Srivastava R, Singh K. Phenolic acid analysis and biological activity of methanolic extracts of some medicinal plants against some phytopathogenic Fungi. Intern J Alternative Med 2008;6:2-10.
8. Raja Charaborthy, Saiket Sen, Manoj Kumar Deka, Rekha B, Sandeep Sachan. Anti microbial evaluation of Saraca indica leaves extracts by disk diffusion method. J Pharm Chem Biol Sci 2014;1:1-5.
9. Winee Surabhi Lall, Amit Alexander Charan, Akhilesh. Bind, Antimicrobial acivity of methanolic and acetonic extracts of Azaridacta indica, Saraca asoca and Curcuma longa. Int J Med Pharm Sci 2013;3:79-86.
10. Umoh AV, Gabriel MA. Genital tuberculosis with secondary infertility-a case report of successful treatment and subsequent livebirth in Uyo, Nigeria. J Med Med Sci 2011;2:839-42.