• Sujatha Peela Dept. of Biotechnology, Dr. B. R. Ambedkar University, Srikakulam, AP, India
  • Swethalatha Porana Dept. of Biotechnology, Dr. B. R. Ambedkar University, Srikakulam, AP, India


Objective: The Objective of this research is to isolate and screen potent antibiotic producing actinomycetes from unexplored regions of Bay of Bengal Near Srikakulam Coast.

Methods: The isolation and screening were done on starch casein agar media. Antimicrobial activity screening was done using cross streak method and agar diffusion method. Further, Morphological characterization of the isolate was done using Scanning electron Microscopy (SEM-JSM 6610). Further, the cultural characterization of the isolates was done using standard protocols.

Results: A total of sixty-eight isolates of Actinomycetes were collected from marine sediments of coastal line of Srikakulam district of Andhra Pradesh, which is one of the longest coastal lines of Andhra Pradesh. Due to its longest coastal line and geographical distribution, there are promising chance of diversity of the microorganisms and antibiotic producing potential. Each isolate was tested against various bacteria. Among all the isolates twelve isolates were promising and showed good antimicrobial activity. Within twelve isolates the isolate A-10 was very promising and showed antibiotic potential against most of the tested bacteria. This isolate appears to produce high anti-bacterial compounds on potato dextrose agar and nutrient agar medium respectively by using the agar diffusion method. The potent Actinomycete A-10 was further characterised by morphological methods consist of macroscopic and microscopic methods. The mycelium structure, colour and arrangement of conidiophores were observed through the oil immersion (100X). The strain was grown on Starch-Casein agar medium for morphological study using Scanning Electron microscopy. Scanning Electron micrographs of the strain A-10 results that the spore chain was straight and with more than 20 spores per chain. The spores are cylindrical in shape and had a smooth surface. Whirls, sclerotic granules, sporangia and flagellate spores were not observed. Further various biochemical tests performed for the identification of potent isolates are as follows: Melanin reaction, H2S production, tyrosine reaction, starch hydrolysis, casein hydrolysis, gelatin hydrolysis, milk coagulation and peptonization, nitrate reduction, temperature range of growth, pH tolerance and cell wall type by comparing all these results with the Bergey's manual of Determinative Bacteriology and the organisms were identified. The isolate was identified as Streptomyces sp.

Conclusion: Thus the result of antibacterial screening represents that the coastal area of Srikakulam is a potential source of antibiotic producing actinomycetes. Further the phylogenic tree construction and purification of the antibiotic principle from A-10 were under investigation.

Keywords: Actinomycetes, Marine Sediments, Starch-Casein agar, SEM


1. Aparanji P, Venkataramana L, Murali Krishna R. Isolation of potent antibiotic producing actinomycetes from marine sediments of Andaman and Nicobar marine Islands. J Microbiol Antimicrobial 2013;5:6-12.
2. Jaganmohan YSYV, Sirisha B, Haritha R, Ramana T. Selective screening isolation and characterization of antimicrobial agents from marine actinomycetes. J Pharam Pharm Sci 2013;5:443-9.
3. Febina Bernice Saron S, Rachel Regi D, Shenbagarathai R. Screening of marine actinomycetes from coastal regions of Tamilnadu. Indian J Appl Res 2013;3:19-20.
4. Sunillaxman A, Gaviraj N, Sifabdulrahimankarigar A, Ravindra K, Nagesh C, Chandra Shekara S. Screening, isolation and marine purification of antibacterial agents from marine actinomycetes. Int Curr Pharm J 2012;1:394-402.
5. Gunasekaran M, Thangavel S. Isolation and screening of actinomycetes from marine sediments for their potential to produce antimicrobials. Int J Life Sci Biotechnol Pharm Res 2014;2:115-26.
6. M Marine, P Arungao, G Ebmer Bon, Maceda, F Marine Angelica, Villano. Screening of antibiotic–producing actinomycetes from marine,Brackish and terrestrial sediments of a small island, Philippines. J Res Sci Computing Eng 2007;4:29-38.
7. Vijayakumar R, Panneerselvam K, Muthukumar C, Thajuddin N, Panneerselavan A, Saravanamuthu R. Optimization of antimicrobial production by marine actinomycetes streptomyces. Afghaniensis VPT53-1 isolated from palk strait, East coast of India. Indian J Microbiol 2012;52:230-9.
8. Benita Mercy R, krishnankanna B. Antimicrobial activity of streptomyces albafaciens against methicillin resistant staphylococcus aureus and vancomycine resistant species. Res J Pharm Biol Chem Sci 2013;4:1248-57.
9. Dubey RC, Maheswari DK. Practical Microliology; 2015. p. 55-7.
10. Chinnanarasaiah B, Leelavathi V, Sudhakar G, Mariyadasu P. Isolation identification and molecular characterization of actinomycetes strain from the laterite soil of kandukuru. Int J Recent Sci Res 2014;5:2236-43.
11. Yaminisudha Lakshmi S, Hharithalaksmi D, Sharmila S. Isolation, screening, identification, characterization and application green synthesised silver nanoparticle from marine actinomycetes streptomycesalthioticus. W J Pharm Res 2015;4:1592-611.
12. Vidyasr N, Yamini Sudha Lakshmi S. Isolation, screening, identification, charecterization and application green synthesized silver nanoparticle from marine actinomycetes(Streptomyces Griese Rubens). W J Pharm Res 2015;4:1801-20.
13. Jensen PR, Gontang E, Mafnas C. Culturable marine actinomycete diversity from tropical pacific ocean sediment. Environ Microbiol 2005;7:1039-48.
14. Rabbani M, Sadeghi HM, Kajro Z. Molecular detection of streptomyces griseus isolated from soil. Pak J Biol Sci 2007;10:3374-9.
15. Valli S, Suvathi S, Aysha O. Antimicrobial potential of actinomycetes species isolated from marine environment. Asian Pac J Trop Biomed 2012;2:469-73.
16. Mojtaba M, Hamed N, Javad H, Aboulghasem R. Screening of antibactetial producing actinomycetes from sediments of Caspian sea. Int J Med Curr Microbiol 2013;2:64-71.
17. Sujatha P, Bapiraju VVSN, Ramana T. Studies on antagonistic marine actinomycetes from the bay of Bengal. World J Microbiol Biotechnol 2005;2:583-5.
18. Kin S, Lam T. Discovery of novel metabolites from marine actinomycetes. Curr Opin Microbiol 2006;9:245–51.
288 Views | 2640 Downloads
How to Cite
Peela, S., and S. Porana. “ISOLATION AND SCREENING OF NOVEL STREPTOMYCES FROM SEDIMENTS OF BAY OF BENGAL NEAR SRIKAKULAM COAST”. International Journal of Current Pharmaceutical Research, Vol. 9, no. 1, Dec. 2016, pp. 40-44, doi:10.22159/ijcpr.2017v9i1.16603.
Original Article(s)