ASSESSMENT ON ANTIMICROBIAL PROPERTIES OF SURFACTIN FROM BACILLUS SUBTILIS ON PROTOPLASTS AND SPHEROPLASTS OF PATHOGENIC BACTERIA


B. Chitra, A. Benno Susai Vijayakumar

Abstract


Objective: The aim of this paper is to assessment on antimicrobial properties of surfactin from Bacillus subtilis on protoplasts and spheroplasts of pathogenic bacteria.

Methods: Bacillus sp were isolated from the polluted soil samples and these isolates were screened for biosurfactin activity using haemolytic activity. The biosurfactant was also studied for their antibiotic activity and the minimum inhibitory concentration was determined by testing the effect of surfactin on the pathogenic microorganisms. The lytic activity of surfactant was also tested on the protoplasts and spheroplasts of the pathogens.

Results: The formation of a clear zone around the colonies and the lysis of erythrocytes shows the antimicrobial and lytic activity of biosurfactants.

Conclusion: The results of the current study reveal that surfactin from B. subtilis possesses significant antimicrobial and lytic activity.


Keywords


Soil Sample, Biosurfactant isolation, Protoplast and spheroplast identification and their lytic activity by biosurfactin

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About this article

Title

ASSESSMENT ON ANTIMICROBIAL PROPERTIES OF SURFACTIN FROM BACILLUS SUBTILIS ON PROTOPLASTS AND SPHEROPLASTS OF PATHOGENIC BACTERIA

Keywords

Soil Sample, Biosurfactant isolation, Protoplast and spheroplast identification and their lytic activity by biosurfactin

DOI

10.22159/ijcpr.2017v9i1.16612

Date

31-12-2016

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 1, 2017 Page: 67-69

Online ISSN

0975-7066

Statistics

34 Views | 68 Downloads

Authors & Affiliations

B. Chitra
Department of Biotechnology, Srimad Andavan Arts and Science College (Autonomous), Trichy-5, Tamil Nadu, India

A. Benno Susai Vijayakumar
Department of Biochemistry, St. Joseph’s College (Autonomous), Trichy-2, Tamil Nadu, India


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