ANTI-ATHEROSCLEROTIC AND LIPID LOWERING EFFECTS OF CINNAMOMUM VERUM IN CHOLESTEROL-FED RABBITS
Objective: The present study was designed to investigate the antihyperlipidemic and anti-atherosclerotic activity of 70% methanolic crude extract of Cinnamomum verum bark in high cholesterol-fed diet rabbits.
Methods: C. verum extract was administered at a dose level of 200 mg/kg and 300 mg/kg (p. o) daily for 120 d to cholesterol-fed rabbits. Lipid profile in serum and histological changes in heart and aorta were investigated. The statistical analysis was carried out by studentâ€™s â€˜tâ€™test.
Results: Plant extract showed a significant decrease in the levels of serum total cholesterol, triglycerides, phospholipids, LDL, VLDL (P â‰¤ 0.001) in a dose-dependent manner in treated animals. HDL ratio improved overwhelmingly as well as the marked decline was also noticed in the atherogenic index after administration with C. verum extract. Histopathological examinations demonstrated less cholesterol deposits in the aorta and significant increase in lumen size of coronary arteries of high cholesterol diet animals given C. verum compared to the high cholesterol diet animals not given C. verum supplement.
Conclusion: The phytochemical analysis of methanol extracts indicated a strong presence of alkaloids, flavonoids, tannins, phenols, saponins and fatty acids may be responsible for the significant hypolipidaemic as well as antiatherosclerotic activity. Our study exhibited that the methanol extract of C. verum bark is a potent hypolipidaemic agent and decreased cholesterol deposition in the aorta and plaque formation process in the coronary artery of high cholesterol diet animals.
2. Harrison D, Kathy KG, Hornig B, Drexler H. Role of oxidative stress in atherosclerosis. Am J Cardiol 2003;91:7Aâ€“11A.
3. Bibave KH, Shenoy PA, Mahamuni SP, Bandawane DD, Nipate SS, Chaudhari PD. Preclinical evaluation methods for screening of anti-atherosclerotic drugs: an overview. Asian J Biomed Pharm Sci 2011;1:1-14.
4. Harauma A, Murayama T, Ikeyama K, Sano H, Arai H, Takano R, et al. Mulberry leaf powder prevents atherosclerosis in apolipoprotein E-deficient mice. Biochem Biophys Res Commun 2007;358:751-6.
5. Niki E. Antioxidants and atherosclerosis. Biochem Soc Trans 2004;32:156-9.
6. Mahmood ZA, Sualeh M, Mahmood SBZ, Karim MA. Herbal treatment for cardiovascular disease: the evidence-based therapy. Pak J Pharm Sci 2010;23:119-24.
7. Tasawar Z, Siraj Z, Ahmad N, Lashari ML. The effects of Nigella sativa (Kalonji) on lipid profile in patients with stable coronary artery disease in Multan, Pakistan. Pak J Nutr 2011;10:162-7.
8. Tanabe H, Yoshida M, Tomita N. Comparison of the antioxidant activities of 22 commonly used culinary herbs and spices on the lipid oxidation of pork meat. Anim Sci J 2002;73:389-93.
9. Vangalapati M, Sree Satya N, Surya Prakash DV, Avanigadda S. A review on pharmacological activities and clinical effects of cinnamon species. Res J Pharm Biol Chem Sci 2012;3:653-63.
10. Joshi K, Awte S, Bhatnagar P, Walunj S, Gupta R, Joshi S, et al. Cinnamomum zeylanicum extract inhibits proinflammatory cytokine TNFÂµ: in vitro and in vivo studies. Res Pharm Biotechnol 2010;2:14-21.
11. El-Baroty GS, Abd El-Baky HH, Farag RS, Saleh MA. Characterization of antioxidant and antimicrobial compounds of cinnamon and ginger essential oils. Afri J Biochem Res 2010;4:167-74.
12. Aggarwal BB. Targeting inflammation-induced obesity and metabolic diseases by curcumin and other nutraceuticals. Annu Rev Nutr 2010;30:173-99.
13. Gupta C, Garg AP, Uniyal RC, Kumari A. Comparative analysis of the antimicrobial activity of cinnamon oil and cinnamon extract on some food-borne microbes. Afri J Microbiol Res 2008;2:247-51.
14. Koppikar SJ, Choudari AS, Snehal A, Vansi S, Kumari S, Chattopadhyay S, et al. BMC Cancer 2010;10:1471-2407.
15. Buyukbalci A, El SN. Determination of In vitro antidiabetic effects, antioxidant activities and phenol contents of some herbal teas. Plant Foods Hum Nutr 2008;63:27-33.
16. Zlatkis A, Zak B, Boyle AJ. A method for the determination of serum cholesterol. J Lab Clin Med 1953;41:486-92.
17. Gottfried SP, Rosenburg B. Improved manual spectrophotometric procedure for determination of serum triglycerides. Clin Chem 1973;19:1077-8.
18. Zilversmit DB, Davis AK. Microdetermination of plasma phospholipids by trichloroacetic acid precipitation. J Lab Clin Invest 1950;35:155-60.
19. Burnstein M, Scholnic MR, Martin R. Rapid method of isolation of lipoprotein from human serum by precipitation of polyamines. J Lipid Res 1970;11:583-7.
20. Friedwald WI, Levy RI, Freidrickson DS. Estimation of concentration of LDL-cholesterol in plasma without preparative of ultra-centrifuge. Clin Chem 1972;28:449-502.
21. Lowry OH, Rosenburg NJ, Jarr AL, Roudall R. Protein measurement with Folin-phenol reagent. J Biol Chem 1951;193:265-75.
22. Hjermann I, Enger SC, Helgeland A, Holme I, Leren P, Trygg K. The effects of dietary changes on high density lipoprotein cholesterol. The oslo study. Am J Med 1979;66:105-9.
23. Chapman MJ, Caslake M, Packard C, McTaggart F. New dimension of statin action on apo-B atherogenicity. Clin Cardiol 2003;26(1 Suppl 1):I-7-10.
24. Wehinger A, Tancevski I, Schgoer W, Eller P, Hochegger K, Morak M, et al. Phospholipid transfer protein augments in THP-1-derived macrophages induced by lipolyzed hypertriglyceridemic plasma. Arterioscler Thromb Vasc Biol 2007;27:908-14.
25. Mahesh T, Sri Balasubashini MM, Menon VP. Photo-irradiated curcumin supplementation in streptozotocin-induced diabetic rats: effect on lipid peroxidation. Therapie 2004;59:639â€“44.
26. Maruthappan VG, Shree KS. Blood cholesterol lowering effect of Adenanthera pavonina seed extract on atherogenic diet induced hyperlipidaemia rats. Int J Pharm Sci Res 2010;1:87-94.
27. Anderson JW. Whole grains protect against atherosclerotic cardiovascular disease. Proc Nutr Soc 2003;62:135-42.
28. Pande VV, Dubey S. Antihyperlipidemic activity of Sphaeranthus indicus on atherogenic diet induced hyperlipidemia in rats. Int J Green Pharm 2009;3:159-61.
29. Adaramoye OA, Nwaneri VO, Anyanwu KC, Farombi EO, Emerole GO. Possible anti-atherogenic effect of Kolaviron (a Garcinia kola seed extract) in hypercholesterolemic rats. Clin Exp Pharmacol Physiol 2005;32:40-6.
30. Latha M, Pari L. Antihyperlipidaemic effect of aqueous extract of Scoparia dulcis in albino rats treated with Streptozotocin. J Herbs Spices Med Plants 2005;11:59-66.
31. Durak I, Ozturk HS, Oclay E, Guven C. Effects of garlic extract supplementation on blood lipid and antioxidant parameters and atherosclerotic plaque formation process in cholesterol-fed rabbits. J Herbal Pharmacol 2002;2:19-32.
32. Dragland S, Senoo H, Wake K, Holte K, Blomhoff R. Human nutrition and metabolism: Several culinary and medicinal herbs are important sources of dietary antioxidants. J Nutr 2003;133:1286-90.
33. Accad M, Smith SJ, Newland DL, Sanan DA, King LE Jr, Linton MF, et al. Massive xanthomatosis and altered composition of atherosclerotic lesions in hyperlipidemic mice lacking acyl CoA cholesterol acyltransferase1. J Clin Invest 2000;105:711-9.
34. Libby P. Changing concepts of atherogenesis. J Intern Med 2000;247:349-58.
35. Prasad NS, Raghavendra R, Lokesh BR, Naidu KA. Spice phenolics inhibit human PMNL 5-lipoxygenase. Prosta-glandins Leukotrienes Essent Fatty Acids 2004;70:521â€“8.
36. Shihabudeen HMS, Priscilla DH, Thirumurugan K. Cinnamon extract inhibits Î±-glucosidase activity and dampens postprandial glucose excursion in diabetic rats. Nutr Metab (Lond) 2011;8:46.
37. Rao PV, Gan SH. Cinnamon: a multifaceted medicinal plant. J Evidence-Based Complementary Altern Med 2014: http:// dx.doi.org/10.1155/2014/642942
38. Rakshit M, Ramalingam C. In vitro antibacterial and antioxidant activity of Cinnamomum verum (Cinnamon) aqueous bark extract in reference to its total phenol content as a natural preservative to food. Int J Biol Biotechnol 2011;8:539-42.
39. Rezaeizadeh A, Zuki ABZ, Abdollahi M, Goh YM, Noordin MM, Hamid M, et al. Determination of antioxidant activity in methanolic and chloroformic extracts of Momordica charantia. Afr J Biotechnol 2011;10:4932-40.
40. Yu SG, Thomas AM, Gapor A, Tan B, Qureshi N, Qureshi AA. Doseâ€“response impact of various tocotrienols on serum lipid parameters in 5-week-old female chickens. Lipids 2006;41:453-61.
41. Borradaile NM, de Dreu LE, Barrett PH, Huff MW. Inhibition of hepatocyte apo-B secretion by naringenin: Enhanced rapid intracellular degradation independent of reduced microsomal cholesteryl esters. J Lipid Res 2002;43:1544-54.
42. Katan MB, Grundy SM, Jones P, Law M, Miettinen T, Paoletti R, et al. Efficacy and safety of plant stanols and sterols in the management of blood cholesterol levels. Mayo Clin Proc 2003;78:965â€“78.
43. Jayaprakasha GK, Jagan Mohan Rao L, Sakariah KK. Volatile constituents from Cinnamomum zeylanicum fruit stalks and their antioxidant activities. J Agric Food Chem 2003;51:4344â€“8.
44. Lee JS, Jeon SM, Park EM, Huh TL, Kwon OS, Lee MK, et al. Cinnamate supplementation enhances hepatic lipid metabolism and antioxidant defense systems in high cholesterol-fed rats. J Med Food 2003;6:183-91.
45. Abidi P, Zhou Y, Jiang JD, Liu J. ERK-dependent regulation of hepatic LDL receptor expression by herbal medicine berberine. Arterioscler Thromb Vasc Biol 2005;25:2170â€“6.
46. Bei WJ, Guo J, Wu HY, Cao Y. Lipid-regulating effect of traditional chinese medicine: mechanisms of actions. J Evidence-Based Complementary Altern Med 2012. http:// dx.doi.org/10.1155/2012/970635.