• Ashwini S.
  • Suresh Babut V.
  • Saritha .
  • Manjula Shantara Shantaram Department of Studies in Biochemistry, Mangalore University, Post Graduate Centre, Chikka Aluvara, 571232, Kodagu, Karnataka, India


Objective: This study was conducted to examine the anticancer activities in the extracts of marine seaweeds Gracilariacorticata.

Methods: The acetone, chloroform, ethanol, methanol and aqueous extracts of collected seaweeds were tested for their anticancer properties in vitro against HeLa cancer cell lines.

Results: The anticancer activity of the seaweed extracts was observed at 24hours, 48 hours and 72 h in which chloroform and ethanol extracts of G. corticata showed a greater activity with an IC50 value of 341.82 µg/ml and 244.7 µg/ml respectively for 48hours. P-values were determined by two-way analysis of variance (ANOVA). The morphology of the treated cells showed a great variation when compared to the control cells. Thus, the in vitro assay indicates that the extracts of seaweeds are the significant source of a noble anticancer agent.

Conclusion: This study also infers that G. corticata could be a potential candidate for cancer therapy in the near future.

Keywords: Seaweeds, Cytotoxicity, HeLa cell lines, Anticancer


1. Chandini SK, Ganesan P, Suresh PV, Bhaskar N. Seaweeds as a source of nutritionally beneficial compounds-a review. J Food Sci Technol 2008;45:1-13.
2. Holdt SL, Kraan S. Bioactive compounds in seaweed; functional food applications and legislation. J Appl Phycol 2011;23:543–97.
3. Liu M, Hansen PE, Lin X. Bromophenols in marine algae and their bioactivities. Mar Drugs 2011;9:1273-92.
4. Kim SK, Ta QV. Potential beneficial effects of marine algal sterols on human health. Adv Food Nutr Res 2011;64:191-8.
5. Misurcova L, Skrovankova S, Samek D, Ambrozova J, Machu L. Health benefits of algal polysaccharides in human nutrition. Adv Food Nutr Res 2012;66:75-145.
6. Jimenez-Escrig A, Gomez-Ordonez E, Ruperez P. Seaweed as a source of novel nutraceuticals: sulphated polysaccharides and peptides. Adv Food Nutr Res 2011;64:325-37.
7. Kim SK, Li YX. Medicinal benefits of sulfated polysaccharides from sea vegetables. Adv Food Nutr Res 2011;64:391-402.
8. Matsuhiro B, Conte AF, Damonte EB, Kolender AA, Matulewicz MC, Mejias EG, et al. Structural analysis and antiviral activity of a sulfated galactan from the red seaweed Schizymeniabinderi (Gigartinales, Rhodophyta). Carbohydr Res 2005;340:2392-402.
9. Xu N, Fan X, Yan XJ, Li X, Niu R, Tseng CK. Antibacterial bromophenols from the marine red alga Rhodomelaconfervoides. Phytochemistry 2003;62:1221-4.
10. Li XC, Jacob MR, Ding Y, Agarwal AK, Smillie TJ, Khan SI, et al. Capstones A and B, which enhance fluconazole activity in Saccharomyces cerevisiae, from the marine green alga, Penicilluscapitatus. J Nat Prod 2006;69:542-6.
11. Harada H, Noro T, Kamei Y. Selective antitumor activity in vitro from marine algae from Japan coast. Biol Pharm Bull 1997;20:541-6.
12. Xu H, Yao L, Sung H, Wu L. Chemical composition and antitumor activity of different polysaccharides from the roots of Actinidiaeriantha. Carbohydr Pol 2009;78:316-22.
13. RA Lincoln, K Strupinski, JM Walker. Bioactive compounds from algae. Life Chem Rep 1991;8:97-183.
14. Adams NM. Seaweeds of New Zealand: An Illustrated Guide. Canterbury University Press: Christchurch, New Zealand; 1994.
15. Fadli M, Aracil JM, Jeanty G, Banaigs B, Francisco C. Novel Meroterpenoids from Cystoseiramediterranea: use of the crown-gall bioassay as a primary screen for lipophilic anti-neoplastic agents. J Nat Prod 1991;54:261-4.
16. Gonzalez AG, Darias V, Estevez E. Chemotherapeutic activity of polyhalogenated terpenes from Spanish algae. Planta Med 1997;44:44-6.
17. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
18. Gross H, Goeger DE, Hills P, Mooberry SL, Ballantine DL, Murray TF, et al. “Lophocladines, bioactive alkaloids from the red alga Lophocladia sp.” J Nat Prod 2006;69:6404.
19. Smit AJ. Medicinal and pharmaceutical uses of natural seaweed products: a review. J Appl Phycol 2004;16:245-62.
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How to Cite
S., A., S. V., S. ., and M. Shantaram. “SEAWEED EXTRACTS EXHIBIT ANTICANCER ACTIVITY AGAINST HeLa CELL LINES”. International Journal of Current Pharmaceutical Research, Vol. 9, no. 1, Dec. 2016, pp. 114-7, doi:10.22159/ijcpr.2017v9i1.16632.
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