SEAWEED EXTRACTS EXHIBIT ANTICANCER ACTIVITY AGAINST HeLa CELL LINES


Ashwini S., Suresh Babut V., Saritha ., Manjula Shantara Shantaram

Abstract


Objective: This study was conducted to examine the anticancer activities in the extracts of marine seaweeds Gracilariacorticata.

Methods: The acetone, chloroform, ethanol, methanol and aqueous extracts of collected seaweeds were tested for their anticancer properties in vitro against HeLa cancer cell lines.

Results: The anticancer activity of the seaweed extracts was observed at 24hours, 48 hours and 72 h in which chloroform and ethanol extracts of G. corticata showed a greater activity with an IC50 value of 341.82 µg/ml and 244.7 µg/ml respectively for 48hours. P-values were determined by two-way analysis of variance (ANOVA). The morphology of the treated cells showed a great variation when compared to the control cells. Thus, the in vitro assay indicates that the extracts of seaweeds are the significant source of a noble anticancer agent.

Conclusion: This study also infers that G. corticata could be a potential candidate for cancer therapy in the near future.


Keywords


Seaweeds, Cytotoxicity, HeLa cell lines, Anticancer

| PDF | HTML |

References


Chandini SK, Ganesan P, Suresh PV, Bhaskar N. Seaweeds as a source of nutritionally beneficial compounds-a review. J Food Sci Technol 2008;45:1-13.

Holdt SL, Kraan S. Bioactive compounds in seaweed; functional food applications and legislation. J Appl Phycol 2011;23:543–97.

Liu M, Hansen PE, Lin X. Bromophenols in marine algae and their bioactivities. Mar Drugs 2011;9:1273-92.

Kim SK, Ta QV. Potential beneficial effects of marine algal sterols on human health. Adv Food Nutr Res 2011;64:191-8.

Misurcova L, Skrovankova S, Samek D, Ambrozova J, Machu L. Health benefits of algal polysaccharides in human nutrition. Adv Food Nutr Res 2012;66:75-145.

Jimenez-Escrig A, Gomez-Ordonez E, Ruperez P. Seaweed as a source of novel nutraceuticals: sulphated polysaccharides and peptides. Adv Food Nutr Res 2011;64:325-37.

Kim SK, Li YX. Medicinal benefits of sulfated polysaccharides from sea vegetables. Adv Food Nutr Res 2011;64:391-402.

Matsuhiro B, Conte AF, Damonte EB, Kolender AA, Matulewicz MC, Mejias EG, et al. Structural analysis and antiviral activity of a sulfated galactan from the red seaweed Schizymeniabinderi (Gigartinales, Rhodophyta). Carbohydr Res 2005;340:2392-402.

Xu N, Fan X, Yan XJ, Li X, Niu R, Tseng CK. Antibacterial bromophenols from the marine red alga Rhodomelaconfervoides. Phytochemistry 2003;62:1221-4.

Li XC, Jacob MR, Ding Y, Agarwal AK, Smillie TJ, Khan SI, et al. Capstones A and B, which enhance fluconazole activity in Saccharomyces cerevisiae, from the marine green alga, Penicilluscapitatus. J Nat Prod 2006;69:542-6.

Harada H, Noro T, Kamei Y. Selective antitumor activity in vitro from marine algae from Japan coast. Biol Pharm Bull 1997;20:541-6.

Xu H, Yao L, Sung H, Wu L. Chemical composition and antitumor activity of different polysaccharides from the roots of Actinidiaeriantha. Carbohydr Pol 2009;78:316-22.

RA Lincoln, K Strupinski, JM Walker. Bioactive compounds from algae. Life Chem Rep 1991;8:97-183.

Adams NM. Seaweeds of New Zealand: An Illustrated Guide. Canterbury University Press: Christchurch, New Zealand; 1994.

Fadli M, Aracil JM, Jeanty G, Banaigs B, Francisco C. Novel Meroterpenoids from Cystoseiramediterranea: use of the crown-gall bioassay as a primary screen for lipophilic anti-neoplastic agents. J Nat Prod 1991;54:261-4.

Gonzalez AG, Darias V, Estevez E. Chemotherapeutic activity of polyhalogenated terpenes from Spanish algae. Planta Med 1997;44:44-6.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.

Gross H, Goeger DE, Hills P, Mooberry SL, Ballantine DL, Murray TF, et al. “Lophocladines, bioactive alkaloids from the red alga Lophocladia sp.” J Nat Prod 2006;69:6404.

Smit AJ. Medicinal and pharmaceutical uses of natural seaweed products: a review. J Appl Phycol 2004;16:245-62.




About this article

Title

SEAWEED EXTRACTS EXHIBIT ANTICANCER ACTIVITY AGAINST HeLa CELL LINES

Keywords

Seaweeds, Cytotoxicity, HeLa cell lines, Anticancer

DOI

10.22159/ijcpr.2017v9i1.16632

Date

31-12-2016

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 1, 2017 Page: 114-117

Online ISSN

0975-7066

Statistics

72 Views | 218 Downloads

Authors & Affiliations

Ashwini S.

Suresh Babut V.

Saritha .

Manjula Shantara Shantaram
Department of Studies in Biochemistry, Mangalore University, Post Graduate Centre, Chikka Aluvara, 571232, Kodagu, Karnataka, India


Refbacks

  • There are currently no refbacks.