Int J Curr Pharm Res, Vol 9, Issue 1, 126-131Original Article


SYNTHESIS, IN VITRO ANTIMICROBIAL ACTIVITY OF SCHIFF’S BASE, AZETIDINONES AND THIAZOLIDINONES

BHAVNABEN D. MISTRY*, KISHOR R. DESAI**, NIGAM J. DESAI**

*Department of Chemistry, B. K. M. Science College, Valsad, **Department of Chemistry, Uka Tarsadia University, Bardoli-Surat
Email: bhavana_mistry11@yahoo.co.in

Received: 01 Oct 2016, Revised and Accepted: 05 Dec 2016


ABSTRACT

Objective: The objective of the present study is to synthesize 3-Chloro-4-[3-(2,4-dichloro-5-fluoro phenyl)-1H-pyrazol-4-yl]-1-(substituted) azetidin-2-one [4a-n] and 2-[3-(2,4-Dichloro-5-fluoro phenyl)-1H-pyraol-4-yl]-3-(substituted phenyl)-1,3-thiazolidin-4-one [5a-n]. The structure of all synthesized compounds were characterized by IR, 1H NMR, 13C NMR and mass spectral studies.

Methods: The titled compounds 3-Chloro-4-[3-(2,4-dichloro-5-fluoro phenyl)-1H-pyrazol-4-yl]-1-(substituted) azetidin-2-one [4a-n] and 2-[3-(2,4-Dichloro-5-fluoro phenyl)-1H-pyraol-4-yl]-3-(substituted phenyl)-1,3-thiazolidin-4-one [5a-n] were synthesized by the reaction of N-{[3-(2,4-dichloro-5-fluoro phenyl)-1H-pyraol-4-yl] methylene } substituted anilin [3a-n] with chloro acetyl chloride and thioglycolic acid respectively. Compounds N-{[3-(2,4-dichloro-5-fluoro phenyl)-1H-pyraol-4-yl] methylene} substituted aniline [3a-n] were synthesized by the reaction of 3-(2,4-dichloro-5-fluoro phenyl)-1H-pyrazol-4-carbaldehyde [2] with primary aromatic amine in alcohol. All compounds were evaluated for their antimicrobial activity.

Results: Compounds 3a,3b,3d,3j,3l,4d,4e,4j,4l,4m,5e,5g,5h,5n exhibited excellent to good antibacterial activity as compared to reference drugs.

Conclusion: In summary, N-{[3-(2,4-dichloro-5-fluoro phenyl)-1H-pyraol-4-yl] methylene } substituted anilin [3a-n], 3-Chloro-4-[3-(2,4-dichloro-5-fluoro phenyl)-1H-pyrazol-4-yl]-1-(substituted) azetidin-2-one [4a-n] and 2-[3-(2,4-Dichloro-5-fluoro phenyl)-1H-pyraol-4-yl]-3-(substituted phenyl)-1,3-thiazolidin-4-one [5a-n] derivatives have been synthesized and characterized. In vitro antimicrobial testing of the compounds was carried out by microdilution Method. Amongst the synthesised compounds, many of them had proven their antimicrobial potency which varies from good to excellent.

Keywords: Schiff’s base, 2-Azetidinone, 4-Thiazolidinone, Antimicrobial activity


INTRODUCTION

Heteroaromatic compounds have attracted considerable attention in the design of biologically active molecules and advanced organic materials. Heterocycles containing nitrogen atoms in the core structure shows a number of pharmacologically and biologically active compounds. Hence, a practical method for the preparation of such compounds is of great interest in synthetic organic chemistry. Structurally, a Schiff's base (also known as imine or azomethine) is a nitrogen analogue of an aldehyde or ketone in which the carbonyl group has been replaced by an imine or azomethine group. Schiff’s bases of pyrazole aldehydes and aromatic amines exhibit a wide range of biological activities such as antifungal [1], antibacterial [2] and antitubercular [3] etc. The biological significance of this class of compounds impelled us to continue working on the synthesis of new schiff’s bases of pyrazole derivatives.

β-Lactam containing antibacterial agents has become an integral part of chemotherapeutic arsenal available to today’s medical practitioners. Although the number of existing agents are quite extensive, but the search for better and more effective drug is still going on. Azetidinones are the very important class of compounds possessing a wide range of biological activities such as antibacterial [4], anti-inflammatory [5], antihyperlipidemic [6], anticancer [7], antimicrobial [8], antitumor [9], antitubercular [10] etc. Furthermore, thiazolidinone derivatives found to possess a wide spectrum of biological activities [11-17].

MATERIALS AND METHODS

Melting points were determined by open capillaries and are uncorrected. The progress of the reaction was checked on aluminium coated TLC plates (E. Merck) using various solvent systems as mobile phase and visualised under iodine vapour. IR-spectra (cm-1) were recorded on a Shimadzu FT-IR spectrophotometer using KBr pellet method. 1H NMR and 13C NMR spectra were recorded on a Bruker DRX-300 NMR instrument, using CDCl3 as solvent and TMS as an internal reference (chemical shifts in δ, ppm). Mass spectra were obtained on an Agilent 6520 (Q-TOF) Mass spectrometer.

Synthesis of (1E)-1-(2,4-Dichloro-5-fluorophenyl) ethanone hydrazone [1]

A mixture of 2,4-dichloro-5-fluoro acetophenone (0.01 mol) and hydrazine hydrate (0.012 mol) was refluxed in round bottom flask containing absolute alcohol (30 ml) for 2 h in the presence of few drops of acetic acid. The content of the flask was cooled to give a solid product which was filtered, washed with water, dried and recrystallized from ethanol as a yellow crystalline solid.

Synthesis of 3-(2,4-dichloro-5-fluoro phenyl)-1H-pyrazol-4-carbaldehyde [2]

To a cold solution of (1E)-1-(2,4-Dichloro-5-fluoro phenyl)ethanone hydrazone (0.015 mol) in DMF (25 ml) was added POCl3 (0.0395 mol) and resulting mixture was stirred at 55-60 °C for 5-6 h [18]. Then the mixture was cooled to room temperature and poured into ice cold water.

A saturated solution of bicarbonate was added to neutralise the solution. The precipitate so formed was filtered, washed with water, dried and recrystallized from ethanol as a yellowish white crystalline solid.

General procedure for the synthesis of N-{[3-(2, 4-dichloro-5-fluoro phenyl)-1H-pyraol-4-yl] methylene} substituted anilin [3a-n]

A mixture of 3-(2,4-dichloro-5-fluoro phenyl)-1H-pyrazol-4-carbaldehyde (0.01 mol), various primary aromatic amine (0.01 mol) and few drops of gla. Acetic acid was refluxed in methanol for six hours. Then the refluxed content was cooled to room temperature and solid separated was filtered, washed with water and recrystallized from acetone.

[3d] IR (KBr cm-1): 3389.81 (-NH), 1567.08 (C=N), 807.10 (C-Cl), 1094.22 (C-F), 1201.49 (-C-N) 1H NMR (CdCl3) δ: 6.972 (-NH), 5.996 (-CH), 7.361-7.572 (Ar-H), 7.696 (CH-Cl), 7.701 (CH-F), 9.747 (-CH=N). 13C NMR: 161.38(C1), 120.09(C2), 129.69(C3), 129.08(C4), 139.48(C5), 115.4(C6), 139.85(C7), 110.05(C8), 133.45(C9), 160.00(C10), 150.02(C11), 122.99(C12), 125.85(C13), 131.88(C14), 125.85(C15), 122.99(C16). Mass (m/z): 368.5 (M), 374.5 (M+6), 257, 230, 205, 164, 138.

[3m] IR (KBr cm-1): 1567.20 (C=N), 807.32 (C-Cl), 1095.34 (C-F), 1023.0 (-OCH3), 1202.57 (-C-N). 1H NMR (CdCl3) δ: 6.996 (-NH), 5.877 (-CH), 7.328-7.535 (Ar-H), 7.697 (CH-Cl), 7.702 (CH-F), 9.750 (-CH=N), 3.800 (-OCH3). 13C NMR: 161.45(C1), 119.99(C2), 129.78(C3), 129.59(C4), 139.45(C5), 114.44(C6), 139.95(C7), 110.05(C8), 134.55(C9), 159.97(C10), 139.45(C11), 123.25(C12), 122.25(C13), 128.08(C14), 115.50 (C15), 153.67(C16).

Mass (m/z): 364 (M), 368 (M+4), 257, 230, 200, 164, 134.

General procedure for the synthesis of 3-Chloro-4-[3-(2,4-dichloro-5-fluoro phenyl)-1H-pyrazol-4-yl]-1-(substituted) azetidin-2-one [4a-n]

Compound N-{[3-(2,4-dichloro-5-fluoro phenyl)-1H-pyrazol-4-yl] methylene}substituted anilin (0.01 mol) was dissolved in 1,4-dioxan (50 ml). To this solution chloro acetyl chloride (0.012 mol) was added drop wise with constant stirring maintaining the temperature below 10 °C and then tri ethyl amine (0.02 mol) was added to it. The mixture was stirred for 2 h. The reacrtion mixture was then refluxed for 9-10 h. The resulting solution was then poured into crushed ice and the product thus obtained was filtered, washed with water and recrystallized from ethyl acetate.

[4a] IR (KBr cm-1): 1730 (C=O), 1203 (CH-N), 760 (C-Cl), 1062 (C-F). 1H NMR (CdCl3) δ: 6.930 (-NH pyrazol), 5.958 (-CH pyrazol), 5.103 (CH-N), 3.885 (CH-Cl), 7.307-7.960 (Ar-H). 13C NMR: 161.79(C1), 120.0(C2), 131.69(C3), 129.31C4), 138.60(C5), 117.17(C6), 143.12(C7), 114.3(C8), 133.18(C9), 60.5(C10), 62.0(C11), 162.08(C12), 140.75(C13), 119.77(C14), 130.69(C15), 123.05(C16), 129.31(C17), 117.17(C18). Mass (m/z): 409 (M), 415 (M+6), 333.5, 246.5, 230, 164, 141.5.

[4j] IR (KBr cm-1): 1712 (C=O), 1205 (CH-N), 758 (C-Cl), 1070 (C-F), 2919 (-CH3). 1H NMR (CdCl3) δ: 6.931 (-NH pyrazol), 5.961 (-CH pyrazol), 5.105 (CH-N), 3.940 (CH-Cl), 7.305-8.049 (Ar-H), 2.529 (-CH3). 13C NMR: 161.59(C1), 120.0(C2), 130.69(C3), 129.5(C4), 139.97(C5), 117.29(C6), 144.15(C7), 114.39(C8), 133.19(C9), 60.05(C10), 62.0(C11), 162.0(C12), 138.0(C13), 120.48(C14), 129.31(C15), 133.38(C16), 129.31(C17), 120.48(C18). Mass (m/z): 424.5 (M), 430.5 (M+6), 333.5, 307, 259, 196, 164.

General procedure for the synthesis of 2-[3-(2,4-Dichloro-5-fluoro phenyl)-1H-pyraol-4-yl]-3-(substituted phenyl)-1,3-thiazolidin-4-one [5a-n]

A mixture of N-{[3-(2,4-dichloro-5-fluoro phenyl)-1H-pyrazol-4-yl] methylene}substituted anilin (0.01 mol), thio glycolic acid (0.01 mol) and anhydrous zinc chloride (0.01 mol) in DMF was refluxed for 11-12 h. The resulting solution was then poured into crushed ice and the product thus obtained was filtered, washed with cold water and recrystallized from methanol.

[5a] IR (KBr cm-1): 1720.20 (C=O), 1260.70 (CH-N), 760.26 (-Cl), 1066.12 (-F).

1H NMR (CdCl3) δ: 6.927 (-NH pyrazol), 5.959 (-CH pyrazol), 5.871 (CH-N), 4.01 (CH2-S), 7.324-7.704 (Ar-H). 13C NMR: 161.41(C1), 119.99(C2), 131.91(C3), 129.70(C4), 139.82(C5), 117.18(C6), 144.65(C7), 108.74(C8), 135.82(C9), 66.1(C10), 34.0(C11), 171.1(C12), 141.75(C13), 126.4(C14), 131.82(C15), 129.30(C16), 131.82(C17), 126.4(C18). Mass (m/z): 408(M), 412 (M+4), 380, 331, 245, 230, 164.

[5e] IR (KBr cm-1): 1733.35 (C=O), 1256.46 (CH-N), 766.17 (-Cl), 1063.31 (-F), 1345.04 (-NO2). 1H NMR (CdCl3) δ: 6.927 (-NH pyrazol), 5.959 (-CH pyrazol), 5.923 (CH-N), 3.975 (CH2-S), 7.323-7.703 (Ar-H). 13C NMR: 161.48(C1), 119.99(C2), 131.91(C3), 129.67(C4), 140.05(C5), 117.18(C6), 144.75(C7), 108.74(C8), 135.82(C9), 66.16(C10), 42.36(C11), 171.2(C12), 131.82(C13), 108.74(C14), 131.91(C15), 119.76(C16), 126.44(C17), 141.75(C18). Mass (m/z): 453 (M), 457 (M+4), 421, 331, 289, 230, 212, 164, 122.

Scheme


Table 1: Physical, characterization data of compound (3a-n), (4a-n) and (5a-n)

S. No. R Mol. formula Mol. Wt. gm/mol M. P. °C Yield %
3a H C16H10N3Cl2F 334 120 68
3b 2-Cl C16H9N3Cl3F 368.5 140 64
3c 3-Cl C16H9N3Cl3F 368.5 120 62
3d 4-Cl C16H9N3Cl3F 368.5 156 65
3e 2-NO2 C16H9O2N4Cl2F 379 152 54
3f 3-NO2 C16H9O2N4Cl2F 379 140 50
3g 4-NO2 C16H9O2N4Cl2F 379 148 56
3h 2-CH3 C17H12N3Cl2F 348 180 0
3i 3-CH3 C17H12N3Cl2F 348 182 58
3j 4-CH3 C17H12N3Cl2F 348 180 60
3k 2-OCH3 C17H12ON3Cl2F 364 210 66
3l 3-OCH3 C17H12ON3Cl2F 364 190 60
3m 4-OCH3 C17H12ON3Cl2F 364 204 64
3n C10H7 (naphthyl)  C20H12N3Cl2F 384  180  56 
4a H C18H11ON3Cl3F 410.5 130 58
4b 2-Cl C18H10ON3Cl4F 445 170 54
4c 3-Cl C18H10ON3Cl4F 445 186 51
4d 4-Cl C18H10ON3Cl4F 445 200 5
4e 2-NO2 C18H10O3N4Cl3F 455.5 160 47
4f 3-NO2 C18H10O3N4Cl3F 455.5 120 46
4g 4-NO2 C18H10O3N4Cl3F 455.5 166 49
4h 2-CH3 C19H13ON3Cl3F 424.5 196 54
4i 3-CH3 C19H13ON3Cl3F 424.5 132 42
4j 4-CH3 C19H13ON3Cl3F 424.5 100 51
4k 2-OCH3 C19H13O2N3Cl3F 440.5 220 44
4l 3-OCH3 C19H13O2N3Cl3F 440.5 202 40
4m 4-OCH3 C19H13O2N3Cl3F 440.5 176 42
4n C10H7 (naphthyl)  C22H13ON3Cl3F  460.5 190  40 
5a H C18H12ON3Cl2FS 408 127 56
5b 2-Cl C18H11ON3Cl3FS 442.5 152 48
5c 3-Cl C18H11ON3Cl3FS 442.5 173 47
5d 4-Cl C18H11ON3Cl3FS 442.5 200 50
5e 2-NO2 C18H11O3N4Cl2FS 453 160 51
5f 3-NO2 C18H11O3N4Cl2FS 453 120 48
5g 4-NO2 C18H11O3N4Cl2FS 453 186 50
5h 2-CH3 C19H14ON3Cl2FS 422 120 58
5i 3-CH3 C19H14ON3Cl2FS 422 142 56
5j 4-CH3 C19H14ON3Cl2FS 422 168 60
5k 2-OCH3 C19H14O2N3Cl2FS 438 260 52
5l 3-OCH3 C19H14O2N3Cl2FS 438 210 50
5m 4-OCH3 C19H14O2N3Cl2FS 438 224 52
5n C10H7 (naphthyl)  C22H14ON3Cl2FS  458 230  42 

Antimicrobial activity

Following common standard strains were used for screening of antibacterial and antifungal activities: E. Coli (MTCC 442), P. Aeruginosa (MTCC 441), S. Aureus (MTCC 96), S. Pyogenus (MTCC 443), C. Albicans (MTCC 227), A. Niger (MTCC 282), A. Clavatus (MTCC 1323). The strains were procured from Institute of Microbial Technology, Chandigarh. DMSO was used as diluents/vehicle to get desired concentration of drugs to test upon standard bacterial strains. Each synthesised drug was diluted for obtaining 2000 microgram/ml concentration, as a stock solution. In primary screening 1000 microgram/ml, 500 microgram/ml, and 250 microgram/ml concentrations of the synthesised drugs were taken. The actively synthesised drugs found in this primary screening were further tested in the second set of dilution against all microorganisms. The drugs found active in primary screening were similarly diluted to obtain 200 microgram/ml, 100 microgram/ml, 50 microgram/ml, 25 microgram/ml, 12.5 microgram/ml and 6.250 microgram/ml concentrations. The highest dilution showing at least 99 % inhibition zone is taken as MIC. The result of this is much affected by the size of the inoculums. Gentamycin, Ampicillin, Chloramphenicol, Ciprofloxacin, Norfloxacin, Nystatin and Griseofulvin were used as a standard. The Comparative activities of the newly synthesised compounds and the control antibiotics on bacterial and fungal strains respectively were summarised in table 2 and table 3.

Excellent to good activity was observed in compounds 4d (against E. Coli, P. Aeruginosa, S. Aureus, S. Pyogenus), compounds 3g, 4e, 5g, 5n (against E. Coli, S. Aureus, S. Pyogenus), compounds 3a, 3b, 3j, 3l, 4j, 4l, 4m, 5e, 5h (against E. Coli, S. Aureus) as well as compounds 3a, 3c, 3f, 3g, 3h, 3j, 3k, 3l, 4c, 4e, 4f, 4g, 4h, 4k, 4l, 4m, 5b, 5e, 5f, 5g, 5i, 5j, 5l, 5m (against C. Albicans). The remaining compounds were found effective at a much higher concentration as compared to the standard drugs.

Table 2: Antibacterial activity of compounds 3a-n, 4a-n and 5a-n

Code E. Coli P. Aeruginosa S. Aureus S. Pyogenus
No. MTCC 442 MTCC 441 MTCC 96 MTCC 443
3a 100 200 250 125
3b 62.5 100 125 200
3c 200 125 250 62.5
3d 250 200 200 200
3e 200 125 250 125
3f 125 125 250 62.5
3g 62.5 200 100 100
3h 200 250 250 250
3i 250 250 500 100
3j 100 200 250 125
3k 250 200 100 125
3l 100 125 100 250
3m 62.5 250 500 500
3n 200 125 250 500
4a 200 250 500 500
4b 100 200 500 250
4c 125 100 250 500
4d 62.5 50 100 100
4e 100 125 250 100
4f 250 100 200 200
4g 500 500 100 200
4h 250 250 500 500
4i 200 200 200 250
4j 62.5 100 200 250
4k 200 250 250 250
4l 100 62.5 62.5 125
4m 62.5 100 200 200
4n 125 100 250 250
5a 200 125 100 125
5b 125 62.5 100 100
5c 200 62.5 100 250
5d 200 250 250 250
5e 100 125 250 500
5f 200 250 200 250
5g 62.5 100 100 62.5
5h 62.5 125 250 250
5i 250 250 250 500
5j 100 125 500 500
5k 250 250 125 100
5l 250 500 500 500
5m 500 250 500 500
5n 100 125 250 100
Ampicillin 100 -- 250 100
Chloramphenicol 50 50 50 50
Ciprofloxacin 25 25 50 50
Norfloxacin 10 10 10 10

Table 3: Antifungal activity of compounds 3a-n, 4a-n and 5a-n

Code C. albicans A. niger A. clavatus
No. MTCC 227 MTCC 282 MTCC 1323
3a 500 250 250
3b 1000 1000 >1000
3c 250 1000 1000
3d >1000 1000 1000
3e >1000 1000 1000
3f 500 500 500
3g 500 500 1000
3h 250 500 500
3i >1000 1000 1000
3j 100 >1000 >1000
3k 500 1000 1000
3l 500 >1000 >1000
3m 1000 500 500
3n >1000 500 500
4a >1000 500 500
4b >1000 500 500
4c 500 1000 1000
4d 1000 1000 500
4e 500 >1000 >1000
4f 500 200 500
4g 250 1000 1000
4h 500 1000 1000
4i 1000 500 500
4j 1000 1000 1000
4k 250 1000 >1000
4l 500 500 1000
4m 500 250 500
4n 1000 >1000 >1000
5a 1000 500 500
5b 500 500 500
5c 1000 1000 1000
5d >1000 >1000 >1000
5e 500 500 500
5f 250 500 500
5g 500 >1000 >1000
5h 1000 500 >1000
5i 250 >1000 >1000
5j 250 1000 >1000
5k 1000 >1000 >1000
5l 500 500 500
5m 200 500 1000
5n >1000 500 500
Nystatin 100 100 100
Greseofulvin 500 100 100

RESULTS AND DISCUSSION

The compounds were synthesised as per scheme

Compound (1E)-1-(2,4-Dichloro-5-fluorophenyl) ethanone hydrazone 1 were synthesized from 1-(2,4-dichloro-5-fluorophenyl) ethanone, which upon reaction with DMF/POCl3 yields 3-(2,4-dichloro-5-fluoro phenyl)-1H-pyrazol-4-carbaldehyde 2. Compounds N-{[3-(2,4-dichloro-5-fluoro phenyl)-1H-pyraol-4-yl] methylene } substituted anilin [3a-n] were synthesized from 3-(2,4-dichloro-5-fluoro phenyl)-1H-pyrazol-4-carbaldehyde 2 and various aromatic amine, which upon cyclization with chloro acetyl chloride and thioglycolic acid yields 3-Chloro-4-[3-(2,4-dichloro-5-fluoro phenyl)-1H-pyrazol-4-yl]-1-(substituted) azetidin -2-one [4a-n] and 2-[3-(2,4-Dichloro-5-fluoro phenyl)-1H-pyraol-4-yl]-3-(substituted phenyl)-1,3-thiazolidin-4-one [5a-n] respectively. The proposed structures of all the synthesised compounds were well supported by IR, 1H NMR, 13C NMR and mass spectral data. The formation of compounds 3a-n was confirmed by the appearance of singlet signal at δ 9.747-9.750 for CH=N system. The 1H NMR spectrum also displayed signals at δ 5.103-5.105 for CH-N of azetidine ring and at δ 4.01-3.975 for CH2-S of thiazolidinone ring system respectively. Aromatic protons were observed in the usual region as multiplet between δ 7.328-7.535, δ 7.305-8.049, δ 7.323-7.703.

CONCLUSION

In summary, N-{[3-(2,4-dichloro-5-fluoro phenyl)-1H-pyraol-4-yl] methylene } substituted anilin [3a-n], 3-Chloro-4-[3-(2,4-dichloro-5-fluoro phenyl)-1H-pyrazol-4-yl]-1-(substituted) azetidin-2-one [4a-n] and 2-[3-(2,4-Dichloro-5-fluoro phenyl)-1H-pyraol-4-yl]-3-(substituted phenyl)-1,3-thiazolidin-4-one [5a-n] derivatives have been synthesized and characterized. In vitro antimicrobial testing of the compounds was carried out by microdilution Method. Amongst the synthesised compounds, many of them had proven their antimicrobial potency which varies from good to excellent. Excellent to good activity was observed in compounds 4d (against E. Coli, P. Aeruginosa, S. Aureus, S. Pyogenus), compounds 3g, 4e, 5g, 5n (against E. Coli, S. Aureus, S. Pyogenus), compounds 3a, 3b, 3j, 3l, 4j, 4l, 4m, 5e, 5h (against E. Coli, S. Aureus) as well as compounds 3a, 3c, 3f, 3g, 3h, 3j, 3k, 3l, 4c, 4e, 4f, 4g, 4h, 4k, 4l, 4m, 5b, 5e, 5f, 5g, 5i, 5j, 5l, 5m (against C. Albicans). The remaining compounds were found effective at a much higher concentration as compared to the standard drugs.

ACKNOWLEDGEMENT

The services of SAIF, CDRI, Lucknow and Micro care Lab Surat is acknowledged for spectral analysis and antimicrobial testing respectively.

CONFLICT OF INTERESTS

Declared none

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How to cite this article

  • Bhavnaben D Mistry, Kishor R Desai, Nigam J Desai. Synthesis, in vitro antimicrobial activity of schiff’s base, azetidinones and thiazolidinones. Int J Curr Pharm Res 2017;9(1):126-131.


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Title

SYNTHESIS, IN VITRO ANTIMICROBIAL ACTIVITY OF SCHIFF’S BASE, AZETIDINONES AND THIAZOLIDINONES

Keywords

Schiff’s base, 2-Azetidinone, 4-Thiazolidinone, Antimicrobial activity

DOI

10.22159/ijcpr.2017v9i1.16634

Date

31-12-2016

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 1, 2017 Page: 126-131

Online ISSN

0975-7066

Statistics

112 Views | Downloads

Authors & Affiliations

Bhavnaben D. Mistry
Department of Chemistry, B. K. M. Science College, Valsad

Kishor R. Desai
Department of Chemistry, Uka Tarsadia University, Bardoli-Surat

Nigam J. Desai
Department of Chemistry, Uka Tarsadia University, Bardoli-Surat


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