ISOLATION OF POTENT HYDROCARBON DEGRADING MICRO-ORGANISMS AND ITS APPLICATION IN BIOREMEDIATION
Objective: Oil spillage has become a global environmental problem as its constituents are toxic, mutagenic and carcinogenic. Natural bioremediation is the only eco-friendly solution to resist its devastating environmental and economic damage. Microbes are used to change harmful substances to non-toxic substances. The current work focuses on the performance of different bacterial species in degrading the oil components like benzene and polycyclic aromatic hydrocarbons (PAH)s.
Methods: Sample was collected from different areas affected by the oil spill in Mumbai that is from the shore of Juhu, Dadar and Manori in form of tar balls and was enriched and isolated on Bushnell and Hass's media containing 1% crude oil as a sole source of carbon. The potent isolates were then identified by standard biochemical tests referring to Bergey's Manual.
Results: Two partially identified strains were Pseudomonas flavescens and Bacillus sp. biofilms of Pseudomonas spp. was prepared on glass matrix to determine its oil degrading efficiency. An indigenous consortium was developed by the assembly of seven isolates of oil-degrading bacteria.
Conclusion: The developed consortium was able to degrade crude oil completely within 4 d. The obtained isolates seemed to have the potential for bioremediation of oil contaminated soil and tar balls which was justified by setting up of a bioreactor.
2. Guru GS, Gohel HR, Panchal MR, Ghosh SK, Braganza VB. Isolation and enrichments of microbes for degradation of crude oil. International Journal of Engineering Science and Innovative Technology 2013:144-7. Doi:10.11159/icesdp16.104
3. George M, Garrit. Bergey's Manual of Systemic Bacteriology. Vol. II. Springer; 2005.
4. Jayashree R, P Rajesh Prasanna, M Krishnaraju. Biodegradation capability of bacterial species isolated from oil contaminated soil. Youth Edu Res Trust 2012;1:140-3.
5. D Dasgupta, R Ghosh, Senagupta. Biofilm-mediated enhanced crude oil degradation by newly isolated Pseudomonas sp. ISRN Biotechnology 2013. http://dx.doi.org/10.5402/2013/250749
6. Jahir Alam Khan, Syed Haran Abbar Rizvi. Isolation and characterization of microbes from oil contaminated sites. Pelagia Res Library 2011;2:455-60.
7. Jyothi K, K Surendra Babu, Nancy Uara K, Anita Kashyap. Identification and isolation of hydrocarbon degrading bacteria by molecular characterization. Helix 2012;2:105-10.
8. Tazeen Hassan Islam, Badal Ghosh, Suvamay Datta. Isolation and identification of petroleum degrading bacteria from soil and water and assessment of their potentiality in bioremediation. IOSR J Environ Sci Toxicol Food Technol 2013;5:55-8.
9. Das TT, Gohel HR, Panchal MR. Determination of crude oil degradation efficiency of glass biofilm of isolated bacterium and fungus. Int Res J Biol Sci 2014;3:67-9.
10. Darin Maliji, Zakia Olama, Hanafy Holail. Environmental studies on the microbial degradation of oil hydrocarbons and its application in Lebanese oil polluted coastal and marine ecosystem. Int J Curr Microbiol Appl Sci 2013;2:1-18.
11. Tambekar DH, PS Bhorse, PV Gadakh. Biodegradation of phenol by native microorganisms isolated from lonar lake in Maharashtra State India. Int J Pharm Res 2012;2:26â€“30.