GC-MS ANALYSIS OF INVASIVE AQUATIC WEED, PISTIA STRATIOTES L. AND EICHHORNIA CRASSIPES (MART.) SOLMS

  • Tulika Tyagi Faculty of Biotechnology, B. B. D. Government P. G. College, Chimanpura, Jaipur, University of Rajasthan, Jaipur Rajasthan State India
  • Mala Agarwal Faculty of Biotechnology, B. B. D. Government P. G. College, Chimanpura, Jaipur, University of Rajasthan, Jaipur Rajasthan State India

Abstract

Objective: To investigate the bioactive components of an invasive aquatic weed, Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms vegetative parts by using Gas Chromatography-Mass Spectrometer (GC-MS).

Methods: The chemical compositions of the ethanol extract of whole plant Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms was investigated using Agilent Technologies GC-MS (GC-7890A, MS 5975C).

Results: The results of GC-MS analysis of the ethanolic extract revealed the existence of 28 phytochemical compounds in Pistia stratiotes L. n-Hexadecanoic acid,-11-Hexadecenoic acid, ethyl ester, Hexadecanoic acid, ethyl ester, Octadecanoic acid, ethyl ester, 2-Cyclopenten-1-one, 5-hydroxy-2,3-dimethyl, L-Glutamine, 2-Pentadecanone, 6,10,14-trimethyl, Linolelaidic acid, methyl ester, 9,12,15-Octadecatrienoic acid, methyl ester,(Z,Z,Z), Nonadecane, 12,15-Octadecadiynoic acid, methyl ester, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, Diisooctyl phthalate, Docosanoic acid, ethyl ester, Stigmasterol, Bis(2-ethylhexyl) phthalate, 1-Monolinoleoylglycerol trimethylsilyl ether, Ethyl iso-allocholate are the major compound.

The ethanolic extract of Eichhornia crassipes (Mart.) Solms contains 43 phytochemical compounds of high and low molecular weight n-Hexadecanoic acid, E-11-Hexadecenoic acid, ethyl ester, Palmitic acid, Phytol, 9,12,15-Octadecatrienal, 9,12-Octadecadienoic acid, ethyl ester, Linolenic acid, ethyl ester, Stearic acid, ethyl ester, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, α-Glyceryl linolenate, 1-Monolinoleoylglycerol trimethylsilyl ether, Linoleic acid, 2,3-bis-(O-TMS)-propyl ester, Stigmasterol, Linolelaidic acid, methyl ester, 9,12,15-Octadecatrienoic acid, ethyl ester, (Z,Z,Z), Ethyl iso-allocholate, Cholesta-22,24-dien-5-ol, 4,4-dimethyl are the major compounds.

These results indicates Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms possess potent antioxidant, anti-inflammatory, anticancer, antitumour, antiarthritic, cancer preventive, antibacterial effects so can be recommended as a plant of phytopharmaceutical importance.

Conclusion: The ethanol extract of Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms proves as a potential source of bioactive compounds of pharmacological importance.

Keywords: Antioxidant, Anti-inflammatory, Anticancer, Antitumour, Antiarthritic

References

1. Bigoniya P, Singh CS, Shukla A. Pharmacognostical and physicochemical standardization of ethnopharmacologically important seeds of Lepidium sativum Linn. and Wrightia tinctoria R. Br. Indian J Nat Prod Resour 2011;2 Suppl 4:464-71.
2. Kirtikar KR, Basu BD. Indian Medicinal Plants. Delhi: Sri Satguru Publications; 2000.
3. Kirtikar KK, BD Basu. The Indian medicinal plants. Dehradun: Oriental Enterprises; 2001. p. 3576-9.
4. Jayanthi P, Lalitha P. Reducing the power of the solvent extracts of Eichhornia crassipes (Mart.) Solms. Int J Pharm Pharm Sci 2011;3 Suppl 3:126-8.
5. Nair V, Kanfer I, Hoogmartens J. Determination of β-sitosterol and stigmasterol in oral dosage forms using high-performance liquid chromatography with evaporative light scattering detection. J Pharm Biomed Anal 2006;41 Suppl 3:731-7.
6. Ogunlesi M, Okiei W, Ofor E, Osibote AE. Analysis of the essential oil from the dried leaves of Euphorbia hirta Linn (Euphorbiaceae), a potential medication for asthma. Afr J Biotech 2009;8:7042-50.
7. Kametani T, Furuyama H. Synthesis of Vitamin D3 and related compounds Med Res Rev 1987;7 Suppl 2:147-71.
8. Senthil J, Rameashkannan MV, Mani P. Phytochemical profiling of ethonolic leaves extract of ipomoea sepiaria (Koenig Ex. Roxb). Int J Innovative Res Sci Eng Technol 2016;5 Suppl 3:3140-7.
9. Wu L, Gao H, Wang X, Ye J, Lu J. Analysis of the chemical composition of Chrysanthemum indicum flowers by GC/MS and HPTLC. J Med Plants Res 2010;4 Suppl 5:421-6.
10. Vohra A, Kaur H. Chemical investigation of medicinal plant Ajuga bracteosa. J Nat Prod Plant Resour 2011;1 Suppl 1:37-45.
11. Sangeetha J, Vijayalakshmi K. Determination of bioactive components of ethyl acetate fraction of Punica granatum Rind extract. Int J Pharm Sci Drug Res 2011;3 Suppl 2:116-22.
12. Duke's Phytochemical and Ethnobotanical Databases (1992-1996). U. S. Department of Agriculture, Agricultural Research Service. Available from: http://phytochem.nal.usda.gov. [Last accessed on 20 Mar 2017]
Statistics
532 Views | 1236 Downloads
How to Cite
Tyagi, T., and M. Agarwal. “GC-MS ANALYSIS OF INVASIVE AQUATIC WEED, PISTIA STRATIOTES L. AND EICHHORNIA CRASSIPES (MART.) SOLMS”. International Journal of Current Pharmaceutical Research, Vol. 9, no. 3, 1, pp. 111-7, doi:10.22159/ijcpr.2017.v9i3.19970.
Section
Original Article(s)