ANTIRADICAL, ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF HARPULLIA ARBOREA (BLANCO) RADLK. (SAPINDACEAE)


Raghavendra H. L., Prashith Kekuda T. R., Karthik K. N., Ankith G. N.

Abstract


Objective: The present study was conducted to investigate antiradical and antimicrobial potential of extract of Harpullia arborea (Blanco) Radlk. (Sapindaceae) leaves obtained by maceration process.

Methods: Antiradical activity of leaf extract was performed by DPPH and ABTS radical scavenging assays. Antibacterial activity of leaf extract was carried out by Agar well diffusion assay. Antifungal activity of leaf extract was carried out by Poisoned food technique.

Results: Leaf extract displayed concentration dependent scavenging of radicals with potent scavenging activity against ABTS radicals (IC50 value 4.26µg/ml) when compared to DPPH radicals (IC50 value 27.26µg/ml). Extract exhibited inhibitory activity against all test bacteria. Marked and least activity was observed against Staphylococcus epidermidis and Escherichia coli respectively. Considerable reduction in the mycelial growth of test fungi was observed in poisoned plates. Curvularia sp. and Alternaria sp. were inhibited to highest and least extent respectively.

Conclusion: In suitable form, the plant can be used to treat oxidative damage, infectious diseases caused by pathogenic bacteria and to manage seed-borne fungi.


Keywords


Harpullia arborea, Maceration, Agar well diffusion, Poisoned food technique, DPPH, ABTS, IC50

| PDF | HTML |

References


Singh B, Singh VN, Sinha BK, Phukan SJ, Borthukar SK. Harpullia arborea (Blanco) radlk.-a new record to Meghalaya. J Non-Timber Forest Prod 2011;18:237-8.

Bhat GK. Flora of South Kanara. Akriti Prints, Mangalore, India; 2014.

Gowri SS, Vasantha K. Solvent based effectiveness of antibacterial and phytochemical derivatized from the seeds of Harpullia arborea (Blanco) Radlk. (Sapindaceae). J Appl Sci Environ Management 2009;13:99-101.

Karuppusamy S, Rajasekaran KM, Karmegam N. Evaluation of phytomedicines from street vendors in Tamil Nadu, South India. Indian J Traditional Knowledge 2002;1:26-39.

Sharma PP, Sawant RJ. Indigenous traditional practices for eco-friendly management of insect/pest in Maharashtra, India. Recent Res Sci Technol 2012;4:21-4.

Widyawaruyanti A, Devi AP, Fatria N, Tumewu L, Tantular IS, Hafid AF. In vitro antimalarial activity screening of several Indonesian plants using HRP2 assay. Int J Pharm Pharm Sci 2014;6:125-8.

Poovapatthanachart R, Thanakijcharoenpath W. A new norhopane from Harpullia arborea. Fitoterapia 2008;79:498-500.

Raghavendra HL, Kekuda PTR, Akarsh S, Ranjitha MC, Ashwini HS. Phytochemical analysis, antimicrobial and antioxidant activities of different parts of Pleocaulus sessilis (Nees) Bremek (Acanthaceae). Int J Green Pharm 2017;11:98-107.

Kekuda PTR, Rakesh KN, Dileep N, Junaid S, Pavithra GM, Gunaga SS, et al. Antimicrobial and antioxidant activity of Anaphalis lawii (Hook. f.) Gamble. Sci Technol Arts Res J 2012;1:8-16.

Rakesh KN, Junaid S, Dileep N, Vinayaka KS, Kekuda PTR, Raghavendra HL. Antibacterial and antioxidant activity of Fahrenheitia zeylanica (Thw.) Airy. Sci Technol Arts Res J 2013;2:27-33.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.

Al Laham SA, Al Fadel FM. Antibacterial activity of various plants extracts against antibiotic-resistant Aeromonas hydrophila. Jundishapur J Microbiol 2014;7:e11370.

Fankam AG, Kuiate JR, Kuete V. Antibacterial and antibiotic resistance modifying activity of the extracts from allanblackia gabonensis, combretum molle and gladiolus quartinianus against gram-negative bacteria including multi-drug resistant phenotypes. BMC Complementary Altern Med 2015;15:206.

Tankeo SB, Damen F, Sandjo LP, Celik I, Tane P, Kuete V. Antibacterial activities of the methanol extracts, fractions and compounds from Harungana madagascariensis Lam. ex Poir. (Hypericaceae). J Ethnopharmacol 2016;190:100-5.

Chung PY, Chung LY, Ngeow YF, Goh SH, Imiyabir Z. Antimicrobial activities of Malaysian plant species. Pharm Biol 2004;42:292-300.

Khan MR, Kihara M, Omoloso AD. Antimicrobial activity of Harpullia ramiflora. Fitoterapia 2001;72:298-300.

Khan MR, Omoloso AD. Antibacterial, antifungal activity of Harpullia petiolaris. Fitoterapia 2002;73:331-5.

Abdelkader MSA, Rateb ME, Mohamed GA, Jaspars M. Harpulliasides A and B: two new benzeneacetic acid derivatives from Harpullia pendula. Phytochem Lett 2016;15:131–5.

Imolehin ED. Rice seedborne fungi and their effect on seed germination. Plant Disease 1983;67:1334-6.

Chandra M, Mahesh NM. Antifungal activity of medicinal plant extracts against seed-borne pathogenic fungi. Acta Biol Indica 2013;2:481-3.

Perello A, Gruhlke M, Slusarenko AJ. Effect of garlic extract on seed germination, seedling health, and vigour of pathogen-infested wheat. J Plant Protection Res 2013;53:317-23.

Sadhu KA. Seed-borne fungi and their effect on seed health of green gram. Biosci Discovery 2014;5:251-5.

Anjorin ST, Mohammed M. Effect of seed-borne fungi on germination and seedling vigour of watermelon (Citrullus lanatus thumb). Afr J Plant Sci 2014;8:232-6.

Pawar BT. Antifungal activity of some seed extracts against seed-borne pathogenic fungi. Int Arch Appl Sci Technol 2015;6:44-6.

Nagpurne VS, Patwari JM. Studies on antimicrobial activity of medicinal plants against seed-borne pathogenic fungi. Int Res J Biol Sci 2016;5:54-6.

Kekuda PTR, Raghavendra HL. Antifungal activity of Helichrysum buddleioides DC. against seed borne fungi. EC Microbiol 2017;6:54-9.

Bharathimatha C, Doraiswamy S, Velazhahan R. Inhibition of fungal plant pathogens by seed proteins of Harpullia cupanioides (Roxb.). Acta Phytopathol Entomol Hung 2002;37:75-82.

Pham Huy LA, He H, Pham Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008;4:89-96.

Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev 2010;4:118-26.

Pawar C, Surana S. Antioxidant properties of the methanol extract of the wood and pericarp of Caesalpinia decapetala. J Young Pharm 2010;2:45-9.

Sudharshan SJ, Valleesha NC, Chinmaya A, Kekuda PTR, Murthuza S, Rajeshwara AN. Radical scavenging activity, phenol and flavonoid content of selected traditionally used Indian medicinal plants. Asian J Exp Sci 2010;24:11-5.

Sivanandham V. Free radicals in health and diseases-a mini review. Pharmacologyonline 2011;1:1062-77.

Alok S, Jain SK, Verma A, Kumar M, Mahor A, Sabharwal M. Herbal antioxidant in clinical practice: a review. Asian Pacific J Trop Biomed 2014;4:78-84.

Pisoschi AM, Pop A, Cimpeanu C, Predoi G. Antioxidant capacity determination in plants and plant-derived products: a review. Oxid Med Cell Longevity 2016. http://dx.doi.org/ 10.1155/2016/9130976

Okawa M, Kinjo J, Nohara T, Ono M. DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity of flavonoids obtained from some medicinal plants. Biol Pharm Bull 2001;24:1202-5.

Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 2004;26:211-9.

Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol 2011;48:412-22.

Kicel A, Wolbiś M. Phenolic content and DPPH radical scavenging activity of the flowers and leaves of Trifolium repens. Nat Prod Commun 2013;8:99-102.

Moustafa SMA, Menshawi BM, Wassel GM, Mahmoud K, Mounier MM. Screening of some wild and cultivated Egyptian plants for their free radical scavenging activity. Int J PharmTech Res 2014;6:1271-8.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 1999;26:1231-7.

Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 2005;53:4290-302.

Biskup I, Golonka I, Gamian A, Sroka Z. Antioxidant activity of selected phenols estimated by ABTS and FRAP methods. Postepy Hig Med Dosw 2013;67:958-63.

Shalaby EA, Shanab SMM. Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian J Geo-Marine Sci 2013;42:556-64.




About this article

Title

ANTIRADICAL, ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF HARPULLIA ARBOREA (BLANCO) RADLK. (SAPINDACEAE)

Keywords

Harpullia arborea, Maceration, Agar well diffusion, Poisoned food technique, DPPH, ABTS, IC50

DOI

10.22159/ijcpr.2017v9i5.22133

Date

21-09-2017

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 5 (Aug-Sep), 2017 Page: 32-36

Online ISSN

0975-7066

Statistics

38 Views | 23 Downloads

Authors & Affiliations

Raghavendra H. L.
Department of Biochemistry, School of Medicine, Wollega University, P. O. Box: 395, Nekemte, Ethiopia
India

Prashith Kekuda T. R.
Department of Microbiology, S. R. N. M. N. College of Applied Sciences, N. E. S. Campus, Balraj Urs Road, Shivamogga 577201, Karnataka, India
India

Karthik K. N.
Department of Microbiology, S. R. N. M. N. College of Applied Sciences, N. E. S. Campus, Balraj Urs Road, Shivamogga 577201, Karnataka, India
India

Ankith G. N.
Department of Microbiology, S. R. N. M. N. College of Applied Sciences, N. E. S. Campus, Balraj Urs Road, Shivamogga 577201, Karnataka, India
India


Refbacks

  • There are currently no refbacks.