OPTIMIZATION AND CHARECTERIZATION OFINTRACELLULAR ORANGE FLUORESCENT PIGMENT FROM BACILLUS ENDOPHYTICUS (AVP-9(Kf527823)


Mantri Sai Ram, Nokku Pradeep Kumar, C. V. S. Bhaskar, Amrutha V. Audipudi

Abstract


Objective: Our study aimed to characterize and optimize the physico-chemical properties which render the high yield of intracellular orange fluorescent pigment (IOFP) and its antibacterial activity against clinical pathogens.

Methods: Intracellular orange fluorescent pigment (IOFP) extracted from Bacillus endophyticus AVP-9(Kf527823) a rhizobacteria of chilli agricultural field using different solvent systems, fractioned by TLC and analyzed for absorption maxima. Purified pigment was screened for antimicrobial activity against clinical pathogen, Optimization of cultural conditions for maximizing the yield of pigment and compared the yield in different broth media.

Results: AVP-9(Kf527823) showed circular, large orange color colonies which fluoresced orange under U. V light,. Intracellular pigment affectively extracted in acetone showed a bright fluorescent orange spot on the TLC plate, and absorption maxima at 493 nm. Antibacterial activity of isolated pigment showed highest zone of inhibition against E. coli and least to Pseudomonas. Maximum yield of the pigment was achieved in modified nutrient brot (MNB)h medium containing 2% w/v nutrient broth containing1% w/v glucose,1% w/v peptone and0.5% methionine at 35 °C and pH 7.0 with 48 h of incubation period. The yield was observed maximum in MNB. Compared to lactose broth, Tryptone soya broth and peptone water.

Conclusion: Bright fluorescent orange pigment of Bacterium avp-9(Kf527823) with absorption maxima at 493 indicating that the pigment shows the characters of carotenoids. Yield maximum in MNB and its potential antibacterial activity needs further investigation for pharmaceutical applications.


Keywords


Fluorescent pigment, Anti bacterial activity, Optimization, Morphological

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About this article

Title

OPTIMIZATION AND CHARECTERIZATION OFINTRACELLULAR ORANGE FLUORESCENT PIGMENT FROM BACILLUS ENDOPHYTICUS (AVP-9(Kf527823)

Keywords

Fluorescent pigment, Anti bacterial activity, Optimization, Morphological

DOI

10.22159/ijcpr.2017v9i5.22142

Date

21-09-2017

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 5 (Aug-Sep), 2017 Page: 67-74

Online ISSN

0975-7066

Statistics

44 Views | 15 Downloads

Authors & Affiliations

Mantri Sai Ram
Department of Microbiology, Acharya Nagarjuna University, Guntur 522510, A. P. India,
India

Nokku Pradeep Kumar
Department of Microbiology, Acharya Nagarjuna University, Guntur 522510, A. P. India
India

C. V. S. Bhaskar
Department of Botany. V. R College, Nellore 523001, A. P, India
India

Amrutha V. Audipudi
Department of Microbiology, Acharya Nagarjuna University, Guntur 522510, A. P. India
India


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