QUANTIFICATION OF PHYTOCHEMICAL CONSTITUENTS AND IN VITRO ANTIOXIDANT ACTIVITY IN THE LEAVES OFCITRUS MEDICA
Objective: In the present investigation, phytochemical assay and in vitro antioxidant activity of ethanol (70%), methanol, ethyl acetate and hexane extract of Citrus medica leaves were carried out.
Methods: The quantification of total phenolic and alkaloid contents were estimated by taking gallic acid and atropine as standard. Inâ€“vitro antioxidant activity of extracts was evaluated by using different free radicals (DPPH, superoxide and free radicals).
Results: Ethanol extract of leaves have more phenolic and alkaloid contents than other extracts. The selected plant extract was produced concentration dependent percentage inhibition of different free radicals and produced maximum activity at a concentration of 1000Âµgm/ml, and there after percentage inhibition was raised gradually to its maximum level with higher concentrations. HPLC analysis revealed the presence of Gallic acid, Catechein, Rutin, Chlorgenic acid, Queracetine and some unknown components which need to be e identified.
Conclusion: Among the four extracts, ethanol extract of C. medica showed good antioxidant activity.
2. Prasad ND, Ganga Rao B, Sambasasiva Rao E, Mallikarjuna T. Quantification of phytochemical constituents and in vitro antioxidant activity of Meusaferrea leaves. Asian Pac J Trop Biomed 2012;12:S539-S542.
3. Olayinka A, Aiyegoro, Anthony IO. Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysumlongifolium. BMC Complementary Altern Med 2010;10:21.
4. Halliwell B. Antioxidants and human diseases: a general introduction. Nutr Rev 1997;55:S44â€“S52.
5. Faraz M, Mohammad K, Narysanna G, Hamid. Phytochemical screening of some species of Iranian Plants. Iranian J Pharma Res 2003;3:77-82.
6. Edeoga HO, Okwu DE, Mbaere BO. Phytochemical constituents of some Nigerian Medicinal plants. Afr J Biotechnol 2005;4:685-6.
7. Daya L, Chothani, Patel NM. Preliminary phytochemical screening, pharmacognostic and physicochemical evaluation of leaf of Gmelinaarborea. Asian Pac J Trop Biomed 2012;12:S1333-S1337.
8. Pietta PG. Flavonoids as antioxidants. J Nat Prod 2000;63:1035â€“42.
9. Meena AK, Kandale A, Rao MM, Panda P, Reddy GA. Review on citron-pharmacognosy, phytochemistry and medicinal uses. Int Res J Pharm 2011;2:14-9.
10. Onyeyirichi I, Ogechi N, Oche O, Jerry U, Gero M. Evaluation of chemical constituents of Citrus medicalimonum leaf essential oils. J Pharm Sci Innov 2014;3:306-9.
11. Satishkumar T, Baskar R. Screening and quantification of phytochemicals in the leaves and flower of Tabernaemontanaheyneana wall-a near the threatened medicinal plant, Indian J Nat Prod Res 2014;5:237-43.
12. Taha S, Alfy El, Mona H, Nemat Z, Yassin, Rehab F, et al. Estrogenic activity of Citrus medica L. leaves growing in Egypt. J Appl Pharma Sci 2012;8:180-5.
13. Faraz M, Mohammad K, Narysanna G, Hamid RV. Phytochemical screening of some species of Iranian Plants. Iranian J Pharma Res 2003;3:77-82.
14. Harborne B. Phytochemical methods: a guide to modern techniques of plant analysis. 3rd editioin. Chapman and Hall, London, England; 1998.
15. Edeoga HO, Okwu DE, Mbaere BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol 2005;4:685-6.
16. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic acid-phosphotungstic acid reagents. Am J Ecol Viticulture 1965;16:144-58.
17. Fazel S, Hamidreza M, Rouhollah G, Mohammadreza VR. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J Pharm Sci 2008;32:17-20.
18. McCord JM, Fridovich I. Superoxide dismutase: an enzymic function for erythrocuprein (hemocuprien). J Biol Chem 1969;244:6049-55.
19. Shang Tse Ho, Yu-Tang Tung, Kai-Chung Cheng, Jyh-Horng Wu. Screening, determination and quantification of major antioxidants from Balanophoralaxiflora flowers. Food Chem 2010;122:584â€“8.
20. Nurain A, Noriham A, Zainon MN Wan, Saidatul SWK, Khairusy SZ. Phytochemical constituents and bioactivities of aqueous extract of aromatic herbs. Int J PharmTech Res 2012;4:1401-6.
21. Wilson K, Walker J. Principle and techniques of biochemistry and molecular biology, Cambridge University Press. Sixth Edition. New York, USA; 2005. p. 486-513.
22. Cheenpracha S, Park EJ, Yoshida WY, Barit C, Wall M, Pezzuto JM. Potential anti-inflammatory phenolic glycosides from the medicinal plant Moringaoleifera fruits. Bioorg Med Chem 2010;18:6598-602.
23. Wet HD, Fouche G, Van Heerden FR. In vitro cytotoxicity of crude alkaloidal extracts of South African menispermaceae against three cancer cell lines. Afr J Biotechnol 2009;8:3332-5.