QUANTIFICATION OF PHYTOCHEMICAL CONSTITUENTS AND IN VITRO ANTIOXIDANT ACTIVITY IN THE LEAVES OFCITRUS MEDICA
Keywords:Alkaloids, Antioxidant, Citrus medica, HPLC, Phenolics
Objective: In the present investigation, phytochemical assay and in vitro antioxidant activity of ethanol (70%), methanol, ethyl acetate and hexane extract of Citrus medica leaves were carried out.
Methods: The quantification of total phenolic and alkaloid contents were estimated by taking gallic acid and atropine as standard. Inâ€“vitro antioxidant activity of extracts was evaluated by using different free radicals (DPPH, superoxide and free radicals).
Results: Ethanol extract of leaves have more phenolic and alkaloid contents than other extracts. The selected plant extract was produced concentration dependent percentage inhibition of different free radicals and produced maximum activity at a concentration of 1000Âµgm/ml, and there after percentage inhibition was raised gradually to its maximum level with higher concentrations. HPLC analysis revealed the presence of Gallic acid, Catechein, Rutin, Chlorgenic acid, Queracetine and some unknown components which need to be e identified.
Conclusion: Among the four extracts, ethanol extract of C. medica showed good antioxidant activity.
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