EVALUATION OF ETHANOLIC ROOT EXTRACT OF PARTHENIUM HYSTEROPHORUS LINN FOR ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY


Pankaj Lohumi, Tirath Kumar, Lipi Nogai

Abstract


Objective: The work is aimed to draw out the health beneficial properties of a weed (Parthenium hysterophorus Linn). The present work is organized to evaluate the antioxidant and anti-inflammatory activity of the ethanolic root extract of Parthenium hysterophorus Linn.

Methods: In the present work the ethanolic extract was determined by using soxhlet apparatus. The antioxidant scavenging activity of this extract was determined by applying three different assay methods: (1) DPPH (1, 1-diphenyl-2-picryl hydrazyl) free radical method. (2) Nitric oxide scavenging assay and (3) Reducing power method. The anti-inflammatory activity was determined by in vivo method i.e. Carrageenan induced rat paw edema.

Results: DPPH radical scavenging activities of the standard antioxidant and extracts were found to be increased in dose dependent manner. The percentage inhibition increases from 4.19% to 97.09% within the concentration range of 10µg/ml to 160µg/ml. Parthenium hysterophorus Linn effectively reduced the generation of nitric oxide radicals from sodium nitroprusside solution in a concentration dependent manner and percentage inhibition increases from 3.53% to 55.21% within the concentration range 10µg/ml to 160µg/ml. All the concentrations of extract significantly showed higher absorbance than the absorbance of control reaction (0.9705) in reducing power assay. A Higher absorbance indicates high reducing power due to the formation of reduced intermediates. Parthenium hysterophorus Linn showed highly significant anti-inflammatory activity at a dose of 200 mg/kg and the lesser effect was observed at 100 mg/kg with the percentage change in paw volume at 0 min, 30 min, 60 min, 90 min and 120 min.

Conclusion: Thus, from above experimental observations, it can be stated that Parthenium is a natural antioxidant and bearing anti-inflammatory activity.

 


Keywords


Parthenium, Ethanolic extract, Antioxidant, Anti-inflammatory activity

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About this article

Title

EVALUATION OF ETHANOLIC ROOT EXTRACT OF PARTHENIUM HYSTEROPHORUS LINN FOR ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY

Keywords

Parthenium, Ethanolic extract, Antioxidant, Anti-inflammatory activity

DOI

10.22159/ijcpr.2017v9i5.22327

Date

21-09-2017

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 5 (Aug-Sep), 2017 Page: 194-197

Online ISSN

0975-7066

Statistics

170 Views | 20 Downloads

Authors & Affiliations

Pankaj Lohumi
Department of Pharmaceutical Sciences, Kumaun University, Bhimtal Campus, Nainital, Uttarakhand, India 263136

Tirath Kumar
Department of Pharmaceutical Sciences, Kumaun University, Bhimtal Campus, Nainital, Uttarakhand, India 263136

Lipi Nogai
Department of Pharmaceutical Sciences, Kumaun University, Bhimtal Campus, Nainital, Uttarakhand, India 263136


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